Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,674,958 A→T 9.4% intergenic (+11/+14) tqsA → / ← pntB autoinducer 2 exporter/pyridine nucleotide transhydrogenase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,9580AT 9.4% 106.5 / 2.2 40intergenic (+11/+14)tqsA/pntBautoinducer 2 exporter/pyridine nucleotide transhydrogenase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (23/13);  new base T (2/2);  total (25/15)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAA  >  NC_000913/1674818‑1675099
                                                                                                                                            |                                                                                                                                             
gggATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCaga                                                                                                                                             >  1:148768/1‑143 (MQ=255)
gggATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCCACGTTCCGGTAATCGATGGCAATAAAGAGTGACGGCCTCAGCaga                                                                                                                                             >  1:328049/1‑143 (MQ=255)
  gATGGTTGTTAGGACCGGTGGGTATGCTGATTACCGTACAGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTACACGATCACAATAAAGAGGGACGGACCCAGCagagc                                                                                                                                           >  2:132923/1‑142 (MQ=255)
     ggTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCg                                                                                                                                        >  2:226835/1‑143 (MQ=255)
      gttgttGGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACGATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGt                                                                                                                                       <  2:777296/143‑1 (MQ=255)
          ttAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAgg                                                                                                                                   >  2:655705/1‑143 (MQ=255)
          ttAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCGCAATGAAGAGTGACGGCCTCAGCAGGGGCCGGCAgg                                                                                                                                   >  2:610609/1‑143 (MQ=255)
           tAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                                                                  <  1:665070/143‑1 (MQ=255)
                 cGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGTATCGCCGTTCTGTTCAGCGTTTGAGGTTAAGAGCGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                                                                            >  1:570777/1‑143 (MQ=255)
                 cGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATACAGAGTGACGGCCTCCGCAGAGGCCCTCCGGGTTAccg                                                                                                                            >  2:455453/1‑141 (MQ=255)
                 cGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                                                                            >  1:287019/1‑143 (MQ=255)
                    tGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCGCAGCAGAGGCCGTCAGGGTGACAGAGc                                                                                                                         >  2:203916/1‑143 (MQ=255)
                         ctgctgcTTTGCGTTCCGTTGACGATTCTTTTCAAACTTCCGCTTTTTCTAACAGCGGGATGTCAAAGCATCGTCGTTCTGTTAAGCGATCTCATTAAAGAGTGTCGGCCTCAGCTGAGGCTGTCAGGGTTACAGAGCTTTCa                                                                                                                    <  2:22677/142‑1 (MQ=255)
                             tgctTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGATGCCGTCAGGGTTACAGAGCTTTCAGGAt                                                                                                                >  2:177048/1‑143 (MQ=255)
                              gctTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAtt                                                                                                               <  1:552074/143‑1 (MQ=255)
                                           ttGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGGCGACAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgg                                                                                                  >  1:683341/1‑143 (MQ=255)
                                           ttGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgg                                                                                                  >  1:733858/1‑143 (MQ=255)
                                             gACAATTAGTGTCAAAATTGCGCGTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCt                                                                                                <  1:88895/143‑1 (MQ=255)
                                                        tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAcc                                                                                     >  1:322624/1‑143 (MQ=255)
                                                        tCAAAATTGCGCTTGAACAAACAACGGGAGGTCATAGCATCGCCGTTCTGTTAAGCGATCCCAACAAAAAACGTCACCCTCAGCAAAGGCCGTCAGGGTTACGCAGCTTTCAGGACTGCATCCCCCCCGGCTTTAGCGTcccc                                                                                     >  2:639432/1‑143 (MQ=255)
                                                           aaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCACTAAAGAGTGACGGCCTCAGCAGAGGCCGTCCGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCaaa                                                                                  >  2:179980/1‑143 (MQ=255)
                                                           aaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGGCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCaaa                                                                                  >  2:195517/1‑143 (MQ=255)
                                                           aaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCaaa                                                                                  <  1:188595/143‑1 (MQ=255)
                                                           aaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCaaa                                                                                  <  1:226835/143‑1 (MQ=255)
                                                            gctggcgcTTGAAGAAGCAGCGGGCGGTCAAGGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAAc                                                                                 <  2:506398/139‑1 (MQ=255)
                                                                 cgcTTGACCAAACCGCGGGAGGTCAAAGCAGCGCCGTTTTGTTAGGCGATCGCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAt                                                                            <  2:773469/143‑1 (MQ=255)
                                                                       aacaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCCTGTgggg                                                                      >  1:629849/1‑142 (MQ=255)
                                                                       aacaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGGGGgt                                                                      >  2:676476/1‑143 (MQ=255)
                                                                          aaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAAAGTGACGGCCTCAGCAGAGGCCGTAAGGGTTACAGAGCTTTCAGGATTGCATCCACGCGGGCTTTGGCGTCACCAAACAGCATGTGGGTGtt                                                                   >  1:591471/1‑143 (MQ=255)
                                                                           aacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCCAACAGCATGTGGGTGctt                                                                  >  1:219621/1‑143 (MQ=255)
                                                                                 gggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg                                                            <  1:549130/143‑1 (MQ=255)
                                                                                   gAGGTCAAAGCGTCGCCGTTCTGTTGAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa                                                          <  2:406617/143‑1 (MQ=255)
                                                                                    aGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag                                                         <  1:655705/143‑1 (MQ=255)
                                                                                       tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACCGAGCTTTCAGGATTGCATCCACGCCGGCCTTGGCGTCACCAAGCAGCAGGTGGGCGTTTCCCTCGGAGAAc                                                      >  1:250936/1‑143 (MQ=255)
                                                                                        cAAGGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCCGGGTTACAGCGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCACACAACATGTGCGCGTCTTCCTTGAAGACCa                                                     >  2:687437/1‑143 (MQ=255)
                                                                                        cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACa                                                     >  1:403036/1‑143 (MQ=255)
                                                                                                                 aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGcc                            >  1:538090/1‑143 (MQ=255)
                                                                                                                    ttgtttaaGGGTGACGGCGTCAGGAGGGGCCGTCAGGGTTACAGAGCTTTCAGGAGTGCGTCCACGCGGGCGGTGGCGTCACCAGACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAgt                         <  2:106612/137‑1 (MQ=255)
                                                                                                                       aTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGtt                      >  1:452913/1‑143 (MQ=255)
                                                                                                                               tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCAGCGAAc              >  2:122128/1‑143 (MQ=255)
                                                                                                                                 aCGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGt            >  1:156110/1‑143 (MQ=255)
                                                                                                                                    gCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTa         <  2:287019/143‑1 (MQ=255)
                                                                                                                                    gCCTCAGCAGAGGCCGGCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTa         <  2:570777/143‑1 (MQ=255)
                                                                                                                                           cagAGGCCGTCAGGGTTAGAGGGCCTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACaa  <  2:4176/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
GGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAA  >  NC_000913/1674818‑1675099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑