| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 100.0% | 116.5 / ‑6.7 | 38 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (19/18); minor base G (0/1); total (19/19) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACC > NC_000913/2132650‑2132921 | tgtGGTAGAGGGTAAACTGCTGATATTTTGTGCTGGCGCGGTCGAATTTGTCTTTGGGCTTGCGGTAAAAGTGGAAGTAGCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCGCTGc < 2:219813/141‑1 (MQ=255) tGGGAGAGGGTAAACAGCTGTTATTTTTGGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGTAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt < 2:358989/143‑1 (MQ=255) ggTAGAGGGTAGACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCGTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc < 2:497565/143‑1 (MQ=255) tAGAGGGTAAACAGCTGATATCCTTTGCTGGCGCGGTCGAACTTGTCTTTGCGCTCGCGGTACAAGTGGAAGTACCCGGAGAATCTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 1:590524/143‑1 (MQ=255) agagTGTGAAGAGCTGCTAGTTTGTGCGGGCGCGGTCGGATTTGTCTTTGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc < 2:263810/143‑1 (MQ=255) agagGGTAAACAGCTGCTATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc < 1:768365/143‑1 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGAAGGCTCTGCGTCGCCtctt > 1:603346/1‑143 (MQ=255) gaagcAGCTGAGACTGTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCGTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct < 2:675663/139‑1 (MQ=255) aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGTTTTCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa < 1:490817/143‑1 (MQ=255) aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAc > 1:345188/1‑143 (MQ=255) gCTGAGATTGGTGGCTGGCGGGGTCGAATTTGGCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc < 2:276598/143‑1 (MQ=255) tttttGCTGGCTCGGGCGAATTTGTCTTTGTGCTTGTGGTACAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGTGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTc < 2:257839/143‑1 (MQ=255) ttttGCTGGCGCGGTCGACTTTGTCTTTGTGCTTGCGGTAAAAGTGGCAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTCATATGCAGGCTCTGCGTCGCCTGTTCTACTTTCCGCAGTTCg > 2:219293/1‑143 (MQ=255) ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCg > 2:440719/1‑143 (MQ=255) ttGCGGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc < 1:316779/143‑1 (MQ=255) cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGAGGAAGTTCCCGGAGAAGTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa < 2:397334/143‑1 (MQ=255) tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCGCGGTTCGCCGTAc > 2:709903/1‑143 (MQ=255) gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACGGTTCGCCGTACTc > 1:453904/1‑143 (MQ=255) gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCACCGTACTc > 1:392308/1‑143 (MQ=255) cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTCCGCCGTCCTccac > 2:384647/1‑143 (MQ=255) aaTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAt > 1:671194/1‑143 (MQ=255) aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGGCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACAACCCTc > 1:775117/1‑143 (MQ=255) cccgctgtgCGTGCGGGAAAGGTGGGAGTAGCCGGAGAAGTTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATGGGTATGCAGGCTCGGCGGCGCTTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCa < 1:693465/138‑1 (MQ=255) ttGTGCTTGCGGTAAGAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 2:784195/143‑1 (MQ=255) ggTAAAAGGGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGACCTGCTTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa < 2:126126/143‑1 (MQ=255) aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGACGTAATGCt > 1:492664/1‑143 (MQ=255) gTACGCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCtt < 1:630093/143‑1 (MQ=255) aCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTc > 1:585327/1‑143 (MQ=255) ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa > 2:103304/1‑143 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTACTCCGGTGCCCCTTTCAGGTCGGGATCGaacaag > 2:114271/1‑142 (MQ=255) tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCAGTTCGGTATCgaacgaac > 1:3366/1‑143 (MQ=255) tCTGCAGTTCACCGTGATTGATATGCAGACTACGCTTGTCCTTGTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa < 2:345292/143‑1 (MQ=255) atcGTGATTGATGTGCAGGGTCTGCGTCGCCCCTGCTCCTGTCCGCGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc < 2:764962/141‑1 (MQ=255) aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCCCCCGCCGCAGGAAGAGGTCGTAATGCTGTGCCGCTTTGACTTCGGTCACGCACACACACTCTGTGCAACACCATGcgc > 2:709444/1‑141 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCAGGCACCCCGTACTCCACCACCCTCCGCCGGAAGATGTCGTACTCCCGTGCCGCTTCCAGTTCGCTATCGCACACACCCTCTTTGAAACGCCATGcccgg > 2:168595/1‑141 (MQ=255) gTGATTGTTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCTACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAgg < 1:424428/143‑1 (MQ=255) tgattgatATGCAGGCTCTGCGTCGCCTCTTATGAATACCAAGGTACGCCGTACTCCACCACCATCCCCAGGAAGATGACGCAACCCTGTCCCACTTTCAGATCGGAAACGAACAAACACTCTCTAAAACACCAAACCCAgac > 2:279052/1‑141 (MQ=255) tatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAcc > 1:253055/1‑143 (MQ=255) | TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACC > NC_000913/2132650‑2132921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |