Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,661,821 C→G 100% C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,8210CG100.0% 139.8 / NA 47C104S (TGC→TCC) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (24/23);  total (24/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGT  >  NC_000913/2661683‑2661958
                                                                                                                                          |                                                                                                                                          
gTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGAttt                                                                                                                                        >  1:74637/1‑143 (MQ=255)
   cgtgcggcgTCGTGATGATGCTGACGACCATACTCATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATc                                                                                                                                     <  2:619757/137‑1 (MQ=255)
    gTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATcg                                                                                                                                    <  1:591919/143‑1 (MQ=255)
     tGGCGCGTCGTGAGTATGCTGAAGAACAGACACATCCAGCACTTCCTGGGTATTAACCAGTTAGCCAAGAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCAAGCCACAGCGCATGTATCAGGGAttgagagc                                                                                                                                   >  2:273363/1‑137 (MQ=255)
       gcgcGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGACAAAAAAAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATcgc‑cg                                                                                                                                >  1:105826/1‑143 (MQ=255)
          cgtcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGCCTTATCGC‑CGccc                                                                                                                             >  1:5359/1‑143 (MQ=255)
                     tGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTACTGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGGGTTTATCGC‑CGCCCTGGCAgccgcc                                                                                                                  >  2:432304/1‑143 (MQ=255)
                      gCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCt                                                                                                                 >  2:247085/1‑143 (MQ=255)
                      gCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCt                                                                                                                 >  1:368718/1‑143 (MQ=255)
                       ctttggacCAGACAACTTCAGCACTTCCTAATTAGTACCGAGTTCGCCTATAGTAAGTTTGTTGAGAAAGCCGGGGAGTCGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCtt                                                                                                                <  2:7061/138‑1 (MQ=255)
                            ggccAGACACGTCCAGCAGTTGCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTAccc                                                                                                           <  2:167518/141‑1 (MQ=255)
                            gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGATGAGAAGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTATAcac                                                                                                           >  2:453593/1‑141 (MQ=255)
                               cAGACACATCCTGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAGCCTGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGa                                                                                                        <  2:636572/143‑1 (MQ=255)
                                      aTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTCCACTGACAACggg                                                                                                 >  1:306528/1‑143 (MQ=255)
                                      aTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACggg                                                                                                 >  1:80712/1‑143 (MQ=255)
                                       tCCAGCACTTCCTGGTGATTGACCAGTTCGCCTAAAGTAATGTTGTGGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGt                                                                                                <  2:447892/143‑1 (MQ=255)
                                       gccAGCACTCCCTGGTTATTAACCAGTGCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGt                                                                                                <  1:170350/142‑1 (MQ=255)
                                        ccAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg                                                                                               <  1:318585/143‑1 (MQ=255)
                                          aGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAGCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGc                                                                                             <  2:624233/143‑1 (MQ=255)
                                          aGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATGTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGc                                                                                             >  1:15815/1‑143 (MQ=255)
                                                 ccTGGTTATTACCCAGTTCGCCTAAATGAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACa                                                                                      <  2:85481/143‑1 (MQ=255)
                                                           aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCaa                                                                            <  2:368718/143‑1 (MQ=255)
                                                             ccAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGCTCGCTCAAATCACGCCACAGACAGTAGAGAAAGGTTTTATCGCACGCCAT‑TCCGGCGCCTTTACCCTGACCACGGGAGGCGTCTACAGCCTCGTCACCg                                                                          >  2:766764/1‑143 (MQ=255)
                                                                  tCGCCTAAAGTAATGTTGTTGAGACAACCGGTGGGACGGTCGCTCAAATCACGCCCCAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCt                                                                     <  2:90220/143‑1 (MQ=255)
                                                                      gtAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATGGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAAtt                                                                 <  1:384944/142‑1 (MQ=255)
                                                                          aGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACGCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATCACtt                                                             >  2:581567/1‑143 (MQ=255)
                                                                               tgttgtTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTCCGCCa                                                        >  2:232119/1‑143 (MQ=255)
                                                                                 tggtggagaAAACCGGTGAGGCGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAAc                                                      <  1:638140/138‑1 (MQ=255)
                                                                                   gttgATAAAGCCGGTGAGACGGTCGCTCAAGGCACGCCACAGCGCGTGGGTCAGCGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACgg                                                    <  2:502210/143‑1 (MQ=255)
                                                                                             ccGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTGTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATgctgct                                          <  1:138008/143‑1 (MQ=255)
                                                                                              cGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGctgctg                                         >  1:763462/1‑143 (MQ=255)
                                                                                                tggtgaCGCTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGAGTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCGACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGc                                       <  2:272574/139‑1 (MQ=255)
                                                                                                gTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCC‑TTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTCATTACTTCGCCCACGGCGATGCGGCGGGCa                                      >  2:86022/1‑143 (MQ=255)
                                                                                                       ggTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTg                                >  2:55736/1‑143 (MQ=255)
                                                                                                        gTCGCTCAGAGCGCGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc                               <  2:570904/143‑1 (MQ=255)
                                                                                                         tCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGcc                              <  1:677399/143‑1 (MQ=255)
                                                                                                            cTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTGACCACGACAGCGAGTGGCACCTGCAGATTCGTCAACGGCGCTAATTACCTGGCCAGCGGCGAGGCTGCCGGCATCGTTGCCTag                           >  2:427113/1‑142 (MQ=255)
                                                                                                             tCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCACCTGCAGACTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTGAGCCTAAc                          >  2:50672/1‑143 (MQ=255)
                                                                                                                   cACGCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATaa                    <  2:67244/143‑1 (MQ=255)
                                                                                                                      gCCACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAacca                 <  1:689101/143‑1 (MQ=255)
                                                                                                                       ccACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCACCGGCGATGCTGCTGGCATCTTTGCCTATGACATAactac                >  1:102582/1‑143 (MQ=255)
                                                                                                                       ccACAGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCAGCCTTTACCCTGACAACGGGTACCAAATATCAATTTGACCCCGATATTTACAACATTCCCAACGGCGATGCTGCAGGCATCATTACCTACCAGATAaccag                >  2:166163/1‑142 (MQ=255)
                                                                                                                        cacaGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAaccacc               >  1:580823/1‑143 (MQ=255)
                                                                                                                          caGCGCGTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTAGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATATTTGCCTAACAGATAACCACGGc             >  1:109059/1‑143 (MQ=255)
                                                                                                                               cgTGGGTCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGt        <  2:74637/143‑1 (MQ=255)
                                                                                                                                    ggcAGGGATTCATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACg   <  1:37086/141‑1 (MQ=255)
                                                                                                                                     tCAGGGATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCAGACAACGGGTGGCATCTAAAGATTCGTCGACGGCGCTAATTACTTCGCCCACGGCGATGCTGCTGACCTCTTTGCCTAACAGATGACCACCTCCTGGCCCACga  >  1:226507/1‑142 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGC‑CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGT  >  NC_000913/2661683‑2661958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑