| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,634 | A→C | 100% | T108P (ACC→CCC) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,634 | 0 | A | C | 100.0% | 132.3 / ‑6.7 | 47 | T108P (ACC→CCC) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (21/25); total (22/25) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTT > NC_000913/3041494‑3041773 | cccAGTGCGACGCCAGAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGccc < 2:100441/143‑1 (MQ=255) ttGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATcg < 2:756437/143‑1 (MQ=255) aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGAATTCGCTAAAGTGCCCATCGCGCCAgacga > 2:78744/1‑143 (MQ=255) aaaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGGGCg > 1:6274/1‑143 (MQ=255) acgtagAGCAATTTACGTCTCTTCCGCGACGGCGACGGCTTCTCATGGATTGACCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAACAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCgtgt < 2:325889/138‑1 (MQ=255) tgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGAGGAGCGTGtgct > 2:670948/1‑143 (MQ=255) gAGCAATTTACGTCTGTTCCGCGACGGCGATGGCGTTGCATGGATTGAACGGCGCCGCGTGCGCGAACCGCAGCTGACTGCACTGAAAAAATCTGCGGTCTACTCTAAAGTCCCCATCGCACCAGACGATGAGCgtgatattc > 2:65823/1‑137 (MQ=255) aTTTACGTCTGTTCCGCGACGGCCATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt < 2:123159/143‑1 (MQ=255) tACGTCTGTGCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGtt < 1:91525/143‑1 (MQ=255) cGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc < 2:347713/143‑1 (MQ=255) tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGCACTGATAAAATATGCGGTATTCTCTTAAGTGACAATAGAGCAAGAAGACGAGCGAGTGCAGCTTGGTGTTGCCg > 1:126097/1‑143 (MQ=255) tGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAACGTGCCCATCGCGCCAGCCAACGAGCGTGTGCTGCTTGGTGTTGCCGGt > 1:400241/1‑143 (MQ=255) ccGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc > 2:282728/1‑143 (MQ=255) gagggCTTGGGAGGGATGGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc < 2:539670/140‑1 (MQ=255) gCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg < 1:111605/143‑1 (MQ=255) tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTCTTCAGGCGcgccccgcgcc > 2:612244/1‑142 (MQ=255) tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACGGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt < 2:469398/143‑1 (MQ=255) ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg > 2:482530/1‑143 (MQ=255) gCATGGATTGAACGGCGCAGCGTGCGAGAACCGCAGCTGACTGAACTGAAGAAATATGCGGTATTCTCTAAAGTGCCCATAGCGCCAGGCGACGAGCATGTGCTGCTTGGCGTTGCCGGTGTTAAGGAGagccgcgcgcagcg > 2:258893/1‑139 (MQ=255) aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa > 1:750619/1‑143 (MQ=255) aTTGAGCGGCGCAGCGTGCGGGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct < 2:23520/143‑1 (MQ=255) gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt < 1:120267/143‑1 (MQ=255) aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGTGCCGGTTTTGAGGCGCGCGCCGCGCTGGCGCATCTCttt > 2:611974/1‑143 (MQ=255) cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTCCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAg > 2:647402/1‑143 (MQ=255) cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGGATTCTCTAAACTGCCCATCGCTCTAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAg < 1:330150/143‑1 (MQ=255) cgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGa > 1:63426/1‑143 (MQ=255) aGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCGATCGCGCCAGACGACGAGCGTATGCTGCTTGGTGTTGCCCGCTTTCGGGCGCACGCCGCGCTGGCAAACCGCTTTAGCgcgag > 2:359884/1‑139 (MQ=255) gcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAGCTCTTTAGCGAACTGCCtt > 2:424474/1‑143 (MQ=255) aCCGCAGCTGACTGAACTGAAAAAATAGGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaag < 1:38222/143‑1 (MQ=255) ggCAGCTGACTTAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaa < 1:323475/142‑1 (MQ=255) cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaa < 1:517379/143‑1 (MQ=255) tgactgaACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg < 1:601995/143‑1 (MQ=255) tgacggaACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg < 1:79472/143‑1 (MQ=255) cgaccgaACTGAAAAAAGATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg < 1:428132/142‑1 (MQ=255) actgaagtgaAAAGATATGCGGTATTCTGTCAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGt < 2:6824/143‑1 (MQ=255) ctgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa < 2:649986/143‑1 (MQ=255) tgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAGTCTCTTTAGCGAACTGCGTTCGAAAGAAAAACAGGTAg > 1:222394/1‑143 (MQ=255) taaactgaaATAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGGGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAg < 2:35783/141‑1 (MQ=255) gaaatgaaAAAATAGGCGGTATTCCCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGACTTCGAAAGAAAAACAGGTAGt < 2:75305/143‑1 (MQ=255) actgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAGAGAAACACAGGTAGTCa > 1:541826/1‑143 (MQ=255) aaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCCAATCTCTTTATCGTAGTGCCTTCGACAGAAAAACAGGTAGTCAacgaa > 1:251007/1‑143 (MQ=255) tatGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGCACGCAAAACAGGCAGTCAAAGAAGgcgc > 2:167362/1‑143 (MQ=255) ggTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCa < 1:282728/143‑1 (MQ=255) tcgctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg < 1:439459/140‑1 (MQ=255) tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGACGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACGTTGCt > 1:309861/1‑143 (MQ=255) aacgTGCCCATCGCGCCAGACGGCGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATg < 2:122135/140‑1 (MQ=255) aGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGt < 1:424474/143‑1 (MQ=255) gTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCGTGGTGTTGCCGGTTTTCAGGCGCGCGCCCCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGtt > 1:259465/1‑143 (MQ=255) | CCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTT > NC_000913/3041494‑3041773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |