| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,264,056 | A→G | 10.1% | I321T (ATC→ACC) | tdcC ← | threonine/serine:H(+) symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,264,056 | 0 | A | G | 10.1% | 110.2 / 3.3 | 48 | I321T (ATC→ACC) | tdcC | threonine/serine:H(+) symporter |
| Reads supporting (aligned to +/- strand): ref base A (23/19); new base G (3/2); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01 | |||||||||||
ATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCA > NC_000913/3263915‑3264198 | gtGCTGGTAGTGTTCAGGGTGCCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCgatgat < 1:759348/142‑1 (MQ=255)aTGCTGATAGTGTTCAGTTTACCCAGCGACCCTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGCCCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGCACGATTTGAAGATAGCCCCGAGGGCggtgat > 1:9947/1‑143 (MQ=255)aTGCTGATAGTGTTCAGTGTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCACACTTCAGGACCAGGCCATTCAGACCTGCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCgatgat < 2:64493/143‑1 (MQ=255)aTGCTGATAGTGTGCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCgatgat < 1:763709/143‑1 (MQ=255) tGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGatgatg < 2:145488/143‑1 (MQ=255) tGCTGATAGTGTTCAGTGTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGatgatg < 1:43741/143‑1 (MQ=255) tgttggtgtTTAGTTTGCCCAGCGACACTTTAGTTTCGTCGCCCGGATAACCAAACTGCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGaa < 1:512058/138‑1 (MQ=255) tGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGaa < 1:643975/143‑1 (MQ=255) aaGTATACCCAGCGCCACTTTAGTTTTGTCGCCTTTATAACCAAACGTCAGGACCAGGCCATTCAGGCCTTCCCGCGTTCCCAGTTAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATAtt < 2:486104/142‑1 (MQ=255) tttACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGCAGCAAGATTTGAAGATAGCCACGAGTGCGATGACGGAAGCCGCATATTCCa > 2:513164/1‑143 (MQ=255) tttACCCAGCGACACTATAGTTTTGGCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCa < 1:79081/143‑1 (MQ=255) tgcccAGCGACACTTTAGTCTTGCCGCCTTGATAACCAAGCTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAgt < 2:438388/141‑1 (MQ=255) ccAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGGTGGAAGCCGCATATTCCAGTGTa > 1:309241/1‑143 (MQ=255) ccAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGGTGGAAGCCGCATATTCCAGTGTa > 1:312587/1‑143 (MQ=255) aGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAAt > 1:298535/1‑143 (MQ=255) cacTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCAAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATcgcgc > 1:227500/1‑142 (MQ=255) cTTTAGTTTTGTCGCCTTTCTAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAAc < 1:669419/143‑1 (MQ=255) tttAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTACAGCGTTCCCAGATAGTAACCGAAGAAAGACTCGAAGAGAGCCACGAGTGCGCGGATGGCAGCCGCATATGGCAGTGTAAACGAGaagg > 2:8955/1‑141 (MQ=255) tAGTTTTGTCGCCTTTACAAACTAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGATCGAAGAATGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACgtt < 2:656539/143‑1 (MQ=255) ttttGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACgttgtt > 1:388290/1‑143 (MQ=255) ttGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGACGCCGCATATTCCAGTGTAATCGCGAACGTTGtttt > 1:497812/1‑143 (MQ=255) ccTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATCGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGGTGGAAGCCGCATATTCCAGTGGAATCGCGAACGGTGTTTTGGTAcc > 1:307629/1‑143 (MQ=255) ccTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTACAGTGTAATCGCGAACGTAGTTTTGATAcc > 2:669152/1‑143 (MQ=255) cTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTGTTGGTAccc > 2:545716/1‑142 (MQ=255) ttATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGt > 1:30774/1‑143 (MQ=255) aCCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATgg > 2:204283/1‑143 (MQ=255) ccAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGa > 1:249393/1‑143 (MQ=255) cAAACCTCAGGACCAGGCCATTCATCCCTTCCAGCGCTCCCAGATAGTCACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATTGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAc < 2:39896/143‑1 (MQ=255) aGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTg < 2:172411/143‑1 (MQ=255) gACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAACGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGt > 1:66074/1‑143 (MQ=255) gACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGt > 1:460668/1‑143 (MQ=255) gACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGGGGt > 2:325148/1‑143 (MQ=255) tCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATaa > 1:542304/1‑143 (MQ=255) cAGCCCTGCCAGCGTCCCCAGATGGTGACCGAGGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAg < 2:542748/143‑1 (MQ=255) cAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAg > 1:685217/1‑143 (MQ=255) aGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTgg > 1:141801/1‑143 (MQ=255) gCGTGCCCAGCTAGTGACGGAAGCAAGATTTGAAGACAGCCACGAGTGGGATGATGGAAGCCGCATATGCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGa < 2:307629/143‑1 (MQ=255) aGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTg < 1:637065/143‑1 (MQ=255) aTAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGGTGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTgcg < 2:309241/143‑1 (MQ=255) aTAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGGTGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTgcg < 2:312587/143‑1 (MQ=255) aGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCt > 1:429575/1‑143 (MQ=255) tGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGCGGCCGAAGACTCCAGCAACACCGAAAACATTTCTCTTCGAGCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCttt > 1:183086/1‑143 (MQ=255) tGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGGAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCttt > 1:451774/1‑143 (MQ=255) ggcggcagaaagaTTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTg < 1:323540/137‑1 (MQ=255) aagaaagaTTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTc > 1:345575/1‑143 (MQ=255) aagaaagaGTTGAAGATAGCTACGAGGGCGATGCTGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTc < 2:774251/143‑1 (MQ=255) aaagaTGTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGc < 2:512224/143‑1 (MQ=255) gaTTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCAt < 2:24074/143‑1 (MQ=255) aGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCAGCCATGTTGGCCGgag > 2:190043/1‑143 (MQ=255) ctcGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAAGCGCTAACGTTGTTTTGGTACCGGTCAGGGACGCAAAGGGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACa < 1:335128/141‑1 (MQ=255) cGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACaga < 2:30774/143‑1 (MQ=255) aGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGt < 2:249393/143‑1 (MQ=255) cGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAAc < 1:44128/143‑1 (MQ=255) cGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGCGGTTAGCCAGCTAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGCAGACCGCGTAAAc > 2:541846/1‑143 (MQ=255) gatgatGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACa < 2:141801/143‑1 (MQ=255) gatgatGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGCGGTTAGCCAGATAAGAAAGCACTGCAATATTCTGCGCTTTGGCTTCCGCCATCTTGGCAGGAGACagaagcaacc > 2:570491/1‑136 (MQ=255) atgatgGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTAGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAACCAg > 2:395836/1‑143 (MQ=255) atgGAAGCGGCATAGTCCAGTGTAATCGCGACCGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCa < 2:527189/143‑1 (MQ=255) | ATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTTCCAGCGTTCCCAGATAGTGACCGAAGAAAGATTTGAAGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCA > NC_000913/3263915‑3264198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |