Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,332,822 G→A 8.2% intergenic (‑87/+40) yhbE ← / ← rpmA inner membrane protein YhbE/50S ribosomal subunit protein L27

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,332,8220GA 8.2% 125.1 / 3.5 49intergenic (‑87/+40)yhbE/rpmAinner membrane protein YhbE/50S ribosomal subunit protein L27
Reads supporting (aligned to +/- strand):  ref base G (20/25);  new base A (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.73e-01

ATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACC  >  NC_000913/3332686‑3332960
                                                                                                                                        |                                                                                                                                          
aTTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGc                                                                                                                                      <  1:234131/143‑1 (MQ=255)
   gCTGTGGTGAGGGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCggg                                                                                                                                   <  1:542275/143‑1 (MQ=255)
   gCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCggg                                                                                                                                   <  2:614483/143‑1 (MQ=255)
   gCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCggg                                                                                                                                   <  1:384511/143‑1 (MQ=255)
   gCTGGGGTGAGTGCCTAAAGTGTGCCGATGCCTGCCTGCTGCGTCATACCGTATTCCTTGCAAAGCGCACTTGCGGCTTAGCGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCggg                                                                                                                                   <  2:518092/143‑1 (MQ=255)
    cTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCgggg                                                                                                                                  <  1:423387/143‑1 (MQ=255)
          tgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCa                                                                                                                            >  1:468655/1‑143 (MQ=255)
           gAGCGCCCAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAACGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCAt                                                                                                                           <  2:693166/143‑1 (MQ=255)
             gcgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCTCCTCAACGTGTTGCGGGGCTTTCATcc                                                                                                                         <  2:202076/143‑1 (MQ=255)
                  aaaaGACTGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTAc                                                                                                                    <  1:282326/143‑1 (MQ=255)
                       aatgccaaggccTGGCTGGTGCTTGACACCGTATTCCCTGCAAAGCGCATTTGCGAATTAACGTCTCACCGCACGCACTTGTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGa                                                                                                               <  2:315946/143‑1 (MQ=255)
                         tgccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCCTTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCACCGTGTTGCGGGGCTTCCATCCGTTAcagcgccc                                                                                                             >  2:82963/1‑136 (MQ=255)
                          gccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAcgc                                                                                                            <  2:756265/143‑1 (MQ=255)
                            caatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCTTTACCGAGACGCGa                                                                                                          >  1:24264/1‑143 (MQ=255)
                                gcgtgcctgcTGCTTCATACCGTGTTCCCTGCAAAGCGCATGTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGaaaaa                                                                                                      <  2:440552/140‑1 (MQ=255)
                                 cctgcctcCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAAc                                                                                                     <  1:585020/143‑1 (MQ=255)
                                  ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACt                                                                                                    >  1:324851/1‑143 (MQ=255)
                                      ctgctgCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCGTTCATCCGTTACAGGGCCGCGAGAAACTTAtt                                                                                                >  2:399696/1‑143 (MQ=255)
                                        gctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCa                                                                                              >  1:186680/1‑143 (MQ=255)
                                          tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGACAAACTTATTCAGc                                                                                            >  1:54766/1‑143 (MQ=255)
                                           gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCt                                                                                           <  2:227259/143‑1 (MQ=255)
                                           gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCt                                                                                           >  1:151291/1‑143 (MQ=255)
                                              tCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCg                                                                                        <  2:324851/143‑1 (MQ=255)
                                                 tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTAGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATg                                                                                     <  2:413175/143‑1 (MQ=255)
                                                              gCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTAc                                                                        <  1:423428/143‑1 (MQ=255)
                                                              gCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGCTGCGGGGCTTTCATCCGTTACCCGGACGCGAAAAACTTATTCAGCCTCGATGCTGAGAACtttcc                                                                        >  2:146179/1‑141 (MQ=255)
                                                                         ttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTGTTCgcg                                                             >  1:299925/1‑141 (MQ=255)
                                                                          tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc                                                            >  1:162280/1‑143 (MQ=255)
                                                                              gATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCttt                                                        <  1:419619/143‑1 (MQ=255)
                                                                               aTTAACTTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTa                                                       <  1:478382/143‑1 (MQ=255)
                                                                                ttAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTTCCGTGACGCGAAAATCTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTaa                                                      <  1:82386/143‑1 (MQ=255)
                                                                                   aCGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGCTGCTGAGCACTTTAAGGTTTTTCGGGCCtcaagctt                                                   >  2:649544/1‑136 (MQ=255)
                                                                                      tCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGa                                                >  2:490166/1‑143 (MQ=255)
                                                                                      gcggaCCATGCGCATTGTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAATACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGa                                                <  2:542351/139‑1 (MQ=255)
                                                                                       cGCACCATGCGGATTTTTACCGGTTATGGAAGGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGaa                                               <  1:171288/143‑1 (MQ=255)
                                                                                            cgggggcATTGTTGCCGGGGATCGAATGGGAAGGGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCa                                          <  2:552969/138‑1 (MQ=255)
                                                                                             aTGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTCACTTCGAATTTCAc                                         >  2:180860/1‑143 (MQ=255)
                                                                                                           cGGTTATCGAATGTAAAAAGCCACGCAACGTGTTGCCGGCCTTGCACCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCtt                           <  1:490673/143‑1 (MQ=255)
                                                                                                             gTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGCAAAACTTATTCAGCTTCGATTCTGATAAATTTACGGCTCTTCGGGCCTTTAACTTCGCAATTCCCCTTACCGTCTGCgttg                         >  2:682562/1‑142 (MQ=255)
                                                                                                             gTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTCGCCGTCCGTTAACTTCGAATTTCACTTTACCGTCTGCTTTa                         >  2:346488/1‑143 (MQ=255)
                                                                                                                 gcgaatcgaatAAGCCCCGCAACGTGTTTCGGGGGTTTCATCCGTTTCCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAATTTTACGGTTTTTCGGGTCTTTAACTTCGGATTGCACTTTACCGTCTGCTTTAGCaa                     <  2:339956/142‑1 (MQ=255)
                                                                                                                  cGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCaaa                    <  1:607129/143‑1 (MQ=255)
                                                                                                                   gAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATCAATTTACGGTTTTTCGGGCCTTTAACTTCGACTTTCACTTTACCGTCTGCTTTAGCAAAc                   >  1:716061/1‑143 (MQ=255)
                                                                                                                    aaTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACa                  >  1:226662/1‑143 (MQ=255)
                                                                                                                      tGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTCCCGTCTGCTTTAGCAAACaga                >  2:426154/1‑143 (MQ=255)
                                                                                                                             aGCCCCGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATGTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGCCTGCTTTAGCAAAGAGAGTGTggc         >  2:241880/1‑142 (MQ=255)
                                                                                                                                cccGCAACGTGTTGCGGGGCTTTCATCCATTACCGAGACGCGAAAAACTTATTCAACTTCCATGCTGATAAAGTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCGCTTTACCCTCCCCTTTACACCACAGAGTGTGGTCAc      >  2:746741/1‑143 (MQ=255)
                                                                                                                                cccGCAACACGTTGCGGGGCTTTTATCCGTTACCGGGACGCGAAACACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGCGCGTGGTCat      >  2:240284/1‑142 (MQ=255)
                                                                                                                                    gaaCGTGGGGCGGGGCTTTCATCCGTTAGCGGGACGCGAAAAACGTAGTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGTCTTTAACTCCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGAcc  <  1:545714/142‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
ATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACC  >  NC_000913/3332686‑3332960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑