| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,340,191 | G→A | 7.6% | intergenic (‑126/‑84) | mlaF ← / → yrbG | intermembrane phospholipid transport system, ATP binding subunit MlaF/putative transport protein YrbG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,340,191 | 0 | G | A | 7.6% | 134.8 / 3.1 | 53 | intergenic (‑126/‑84) | mlaF/yrbG | intermembrane phospholipid transport system, ATP binding subunit MlaF/putative transport protein YrbG |
| Reads supporting (aligned to +/- strand): ref base G (26/23); new base A (2/2); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01 | |||||||||||
CCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGTTTACAGTGCCGA > NC_000913/3340050‑3340333 | ccACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGc > 1:609367/1‑143 (MQ=255) acaGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCGTTTGTGTTACTTTGTAGCGGCAt > 1:49026/1‑143 (MQ=255) tGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACCTATTTACAGAAGATTACCCGCCGTGGTGAGCGAAAGCTGGCACTTGTTTTACTTTTTAGGCGCATAAAggc > 2:610627/1‑141 (MQ=255) ccATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCaaaa < 2:535358/143‑1 (MQ=255) aaTTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTaa < 1:581576/143‑1 (MQ=255) tCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTGCCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACgg > 1:471575/1‑143 (MQ=255) gcTTTCGTTGATTTTCTAAGCAGGGCGCTCAAGTTACCCTTGAATCCAACGTATTTACAGCATATTACCTGCCGTGGTTAGCGAAAGCTGGCATGTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACgg < 2:92100/142‑1 (MQ=255) gCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATCTTACCCGCAGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCttt > 1:561111/1‑143 (MQ=255) gTTGATTTTCTAATCCTGGCGCTCAATTTAATCTTGCACCCAACATATTTACAGACTATTACCCGCCGTGGTTAGCGCAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCt < 2:135362/143‑1 (MQ=255) gTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTCGCGAAAGCTGGCCTTTGTTTTACTTTTTAGCCGCATAAAGTCACACTTACGCATCCGTTACGGCTTTCt > 1:779973/1‑143 (MQ=255) ttGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTg > 2:18417/1‑143 (MQ=255) gATTTTCTAGGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGaa < 1:636511/143‑1 (MQ=255) gATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTCGCCGCATAAAGTCAAAATTAAGCATCCGTTCCGGCTTTCTGaa > 1:167349/1‑143 (MQ=255) aTTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGaaa > 1:273110/1‑143 (MQ=255) tttCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCACATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAAt > 1:598768/1‑143 (MQ=255) tttCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCACATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAAt > 1:598747/1‑143 (MQ=255) tCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCt > 1:126798/1‑143 (MQ=255) atgCATGGCGTTCCATTTAACCGTGGACCCACCATCTTACCAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCa < 1:517661/141‑1 (MQ=255) cgcTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTCCTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCg > 2:437765/1‑143 (MQ=255) cTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGc > 1:310227/1‑143 (MQ=255) aTTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGt < 1:37447/143‑1 (MQ=255) aaCCTTGAACCCAACTTATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATAc < 2:668882/143‑1 (MQ=255) ttGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTTAGCCGACCGGCGAGTATACCTga > 2:124958/1‑143 (MQ=255) ttGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTga > 1:100716/1‑143 (MQ=255) ttGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTga > 2:697833/1‑143 (MQ=255) cccAACATGTTTACAGAATAGTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGaaa < 1:694014/143‑1 (MQ=255) ccAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTaa < 2:284333/85‑1 (MQ=255) ccAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTaa > 1:284333/1‑85 (MQ=255) aaCATTTTGTCAGAATATTGCCCGCCGTGGGTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCAGAAAGTCAAAATTAAGCATCCGTTACGGCTTTCGGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGa < 2:714077/143‑1 (MQ=255) atatTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCACATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGt < 2:598747/143‑1 (MQ=255) atatTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCACATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGt < 2:598768/143‑1 (MQ=255) tatCTACAGAATATTACCCGCCGTGGTTAGCGCAAGCTAGCATCTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATTCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGtt < 1:742315/143‑1 (MQ=255) tttACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGCTAg > 2:182831/1‑143 (MQ=255) ttcctgAATATGACCCTCCCTGCGTAGCCAAAGTCGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTTAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGa < 1:520813/138‑1 (MQ=255) tACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGAt < 2:273110/143‑1 (MQ=255) cAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGc > 1:598120/1‑143 (MQ=255) gAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCAGCATCATGTCAAAATTAAGCATCCGTTACGGCTTTCTGCAACTCTTCAGTCGGCCGACGATTTTACCTGAAGCAAGGACGTTGCATGCtt > 2:310822/1‑143 (MQ=255) atatTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCtttt < 1:18417/143‑1 (MQ=255) aCCCGCCGTGGTGAGCGAAAGCTGGCATTTGTTTGACTTGTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTa < 2:782542/143‑1 (MQ=255) cgTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTGAGCTACGGCAc > 2:783700/1‑143 (MQ=255) gTGGTTAGCGTAAGCTGGCATGTGTTTTACTTTTTAGCCGAATATTGTTAGTGTTGGTGGTCTGTCGGGCGGTGTTGAAAATCTTCAGCCGACTGTCGAGTATACCTGAAGAAAGTACGTTAGATGCTTTTAGCTACGGCACt < 2:165752/143‑1 (MQ=255) tAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCAGCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTaa < 1:663580/143‑1 (MQ=255) aGCGAAAGCTGGCATTTGTTTTACTTTTTAGCGGCATAAAGTCAAAATTAAGCATCCGTTATGGCTTGCTGAAAATCTTCAGCGGAGCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCGACGGCACTGGTAAt > 2:230212/1‑143 (MQ=255) cGAAAGCTGGCATTTGTTTTACTTTTTATCCGCCTAAAGTCAAAATTAAGCATCCGTTACGGCTTTCGGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAGAGGAAGTACCATGCTTTTAGCTACAGCACTGTTaaatc > 2:627133/1‑140 (MQ=255) aTTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGACTTTCTGAAAATCTTAAGCGGACGGGAGGGTATAGGTGAAGAAAGGAGGTTTGATGCTGTTATCTACGGCACTGTTAATTGTTGGTTTGCtt > 1:782127/1‑143 (MQ=255) tttGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACttt < 1:640206/143‑1 (MQ=255) tttttACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTgg < 2:95006/142‑1 (MQ=255) ttttACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGt < 2:100716/143‑1 (MQ=255) ttttACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGt > 1:601276/1‑143 (MQ=255) tttttAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGTTTa > 2:42132/1‑143 (MQ=255) tttAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGTTTACa < 1:364365/143‑1 (MQ=255) ccGCATAAAGTCACAATTCAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGTTTACAGTGcc < 2:328584/143‑1 (MQ=255) ccGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGTTTACAGTGcc > 1:723248/1‑143 (MQ=255) gCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGTTTACAGTGCCGa < 2:206359/143‑1 (MQ=255) | CCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGTTTACAGTGCCGA > NC_000913/3340050‑3340333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |