Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,477,878 T→A 10.2% intergenic (+20/+29) slyX → / ← slyD protein SlyX/FKBP‑type peptidyl‑prolyl cis‑trans isomerase SlyD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,8780TA10.2% 94.4 / 3.5 40intergenic (+20/+29)slyX/slyDprotein SlyX/FKBP‑type peptidyl‑prolyl cis‑trans isomerase SlyD
Reads supporting (aligned to +/- strand):  ref base T (14/21);  new base A (2/2);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

TGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATC  >  NC_000913/3477741‑3478017
                                                                                                                                         |                                                                                                                                           
tGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTcccc                                                                                                                                        <  1:257003/143‑1 (MQ=255)
    aTGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCtt                                                                                                                                    <  1:733130/143‑1 (MQ=255)
     tGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttt                                                                                                                                   <  1:639603/143‑1 (MQ=255)
       cagaCGGCGAAACCCCGCGCTCATCTGCGTCTGCTGACCGAGAAGTTCCAAGCCAGCCCGCCGTCGAACATCGCGTCGCACGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCttttt                                                                                                                                 <  1:5456/142‑1 (MQ=255)
         ggcggCGCAGCTCCGCGGTCATCTGCGTCTGCTGACCGGGGAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttttttt                                                                                                                               <  2:680833/140‑2 (MQ=255)
                   cggcgcGATCATCTGCGTCCGCGGACGGAGAAGTTAAACGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTc                                                                                                                     <  2:202535/141‑1 (MQ=255)
                             atcTGCGTCTGCTGTGCGGGAAGTTAAAAGCGCGCGCGCCTTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTg                                                                                                           <  2:440232/143‑1 (MQ=255)
                             atcTGCGTCTGCTGACCGAGAGGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTg                                                                                                           <  2:666724/143‑1 (MQ=255)
                               cTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGGAAAAAAAAAGGGGAAAACCAGCTGTTTTGTCACTTTTTCGGTATAAGTGgc                                                                                                         >  1:741513/1‑143 (MQ=255)
                                 gCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaa                                                                                                       <  1:50059/143‑1 (MQ=255)
                                     ctgctgACCGAGAAGTTAAAAGCCAGCCAGCCGTCGCAAATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaaccgc                                                                                                   <  1:490268/143‑1 (MQ=255)
                                            ccGAGGAGTTAAAAGCCAGCCAGCCTTCGACCATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCTGTATTAGTGGCAACCGCAAccgcc                                                                                            <  2:705220/143‑1 (MQ=255)
                                              gagaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGGATTAGTGGCAACCGCAACCGCCGt                                                                                          >  1:456238/1‑143 (MQ=255)
                                               agaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGCAGAAAAGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTGTTTGCTTAATAATCATTTTTTCGGAAAAAGAAGCAACCACAACCGCCGtt                                                                                         >  1:291494/1‑143 (MQ=255)
                                                    ttAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCtt                                                                                    >  1:450947/1‑143 (MQ=255)
                                                      aaaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAACAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTa                                                                                  >  1:257142/1‑143 (MQ=255)
                                                                       cGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTc                                                                 >  1:462583/1‑143 (MQ=255)
                                                                       cGAACATCGCGTCGCAGGCTGAAGAAACGACACCGCGACATTAGTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTc                                                                 <  1:399300/143‑1 (MQ=255)
                                                                         gaCATCGCGCCGCTGGCGGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGc                                                               <  1:696645/142‑1 (MQ=255)
                                                                            aTCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCAc                                                            <  1:474067/143‑1 (MQ=255)
                                                                               gcgTCGCAGGCTGAAGAAACGCTACCGCCACATTAGTGAGCCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCgt                                                         <  1:209768/143‑1 (MQ=255)
                                                                                     cAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCCTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCAt                                                   >  2:376577/1‑143 (MQ=255)
                                                                                        gCTAAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTCGTCACTTTTTCGGTATTAGTGGCAACCGCATCAGCCGTCGCCTTTACCGCCACAGCAGCGTTCGCAACCGCGTTCagggc                                                >  2:223912/1‑139 (MQ=255)
                                                                                            aagaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGTTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTg                                            >  1:754288/1‑143 (MQ=255)
                                                                                              gaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTCTTAGTGGCAACCGCAACCGCCGTTGCCTCTACCGCCACAGCAGCCTCCGCCACCATGTTCATGACCgcgcc                                          >  2:435866/1‑139 (MQ=255)
                                                                                               aaaCGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTAATAGTGGCAACCGCAACGGGCGTTGCCGTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATc                                         >  1:614131/1‑143 (MQ=255)
                                                                                                 aCGCCACCGCCACATTATCGAGGCGTAAAAAAAGCAGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGt                                       <  1:191208/143‑1 (MQ=255)
                                                                                                  cgccaccgtcacATTATTGAGGCGCAAAAAGAGCGGGGATCCCCCGCTTTTTTGTCCCTTTTTCTGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTg                                      <  2:636210/143‑1 (MQ=255)
                                                                                                        cgccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCTGCCTTCGCCACCGTGTTCATAACCGTGATCGTGGCCAta                                >  1:195805/1‑142 (MQ=255)
                                                                                                            acaTTATTGCGGCGTAAAAAAAGCGTGGAGTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGAccg                            <  2:292462/143‑1 (MQ=255)
                                                                                                                tattGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCACCCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACcgccgc                        <  2:749083/143‑1 (MQ=255)
                                                                                                                 attGAGGCGTAAAAAGTGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCgca                       <  2:8989/143‑1 (MQ=255)
                                                                                                                     aGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTGACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATAGTGGCCATGACCGCCGCAGCaa                   >  1:507868/1‑143 (MQ=255)
                                                                                                                     aGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCaa                   >  1:552693/1‑143 (MQ=255)
                                                                                                                            aagctggCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACcgtcgt            <  2:257142/137‑1 (MQ=255)
                                                                                                                              aaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCATTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTCATCGTGGCCATGACCGCCGCAGCAACCgacgtgg          >  2:213237/1‑143 (MQ=255)
                                                                                                                                   cGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgtg     <  1:432503/143‑1 (MQ=255)
                                                                                                                                     gggATTCCCCGCTTTTTGGTCACTTTTGCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGAt   <  2:430165/143‑1 (MQ=255)
                                                                                                                                      ggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGGCGTGGTCGTGATc  >  1:83710/1‑143 (MQ=255)
                                                                                                                                      ggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCATGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATc  <  1:30302/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATC  >  NC_000913/3477741‑3478017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑