| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,959,515 | T→A | 21.6% | intergenic (+70/+17) | ilvC → / ← ppiC | ketol‑acid reductoisomerase (NADP(+))/peptidyl‑prolyl cis‑trans isomerase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,959,515 | 0 | T | A | 21.6% | 56.8 / 12.9 | 32 | intergenic (+70/+17) | ilvC/ppiC | ketol‑acid reductoisomerase (NADP(+))/peptidyl‑prolyl cis‑trans isomerase C |
| Reads supporting (aligned to +/- strand): ref base T (15/10); new base A (3/4); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01 | |||||||||||
AGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAA > NC_000913/3959384‑3959657 | aggcaggtAAGCGGCTGCGCGGCTATCTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt < 2:551138/143‑1 (MQ=38) ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCtt < 1:594004/143‑1 (MQ=255) gtaggtaAGAAACTGCGGGGGTATAGGACAGATAGGAAACGTATTGCTGTTGCGGGTTAAGAGCGCGCTGATGCCCTCACCCCGACCCTCTCAAAAAAAAAAAAAAAAAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTg < 2:753637/143‑1 (MQ=255) aggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGGAAACGCTCAAGGCCTTCTCCTGGAGAAGGCGTTGcg > 1:189879/1‑142 (MQ=255) aggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTgag < 1:165853/143‑3 (MQ=255) aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCAGGAGAAGGCCTTgagtgtt < 1:468387/143‑7 (MQ=255) aCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc < 2:570089/143‑1 (MQ=255) gtGCGCGGCGATAGGACAGAGATGAGACGTATTGCGGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCGCCCACAGGGAGAGGGAGAAAACACTCGAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg < 2:641382/142‑1 (MQ=37) aTTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGCAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGaa > 1:678309/1‑143 (MQ=255) tGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGCAGGCCTCGCTATTCGTTGCGGTACAGCCCCTCAACGATGGGATATCCGACCTgga > 1:693694/1‑142 (MQ=37) tGCGCGGTGATGCCCTCACCCCGACAATCTTCGGCCGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg < 1:211496/143‑1 (MQ=255) ctctCCCCGGTCCTCTCCCACAGGGAGCGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATCAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAc < 2:673905/143‑1 (MQ=255) cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGAc > 1:786017/1‑143 (MQ=255) ccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAgaga > 1:160155/1‑143 (MQ=255) ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGAACCTTAATGATGTGATATCCGAACTGGGTCTTCTGCCGGCCGGTCGGCTCCAGTCCCGGACAGACGAAAACCa > 1:225829/1‑143 (MQ=255) ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCAATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGCGCGCGAAGCGGGCTGCTCGGCTCCAGCACCGGACAAGCCAACACCa > 2:163503/1‑143 (MQ=255) ccACAGGGAGAGGGAGAAAACACTCAAGGCCATCTACAAGAAGATGCCTTGCTATTAGTTGCGGTACAGCCCCATAAGCCTGTGATATCCGAACTTGGTGTGCAGCGGGCCGGTCTGCGCCAGTACCTGACAAGAGAAGACCa > 2:597841/1‑143 (MQ=255) gcagaggcacaAAACACTCACCGCCGTCTCCTCGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAGGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTAt < 2:574268/141‑1 (MQ=255) agagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCg > 1:45889/1‑143 (MQ=255) agggagaAAACGCTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTTATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGaa < 1:10138/143‑1 (MQ=255) gagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGc > 1:348024/1‑143 (MQ=255) aaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCAGGCCGGTCGGCTCCAGTAGCGCACAAGAGAAAACCACTTCATCGAAAGCCGGa > 2:363735/1‑143 (MQ=255) taatagcAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGcc > 1:453498/7‑139 (MQ=255) taatagcAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGcc < 2:453498/133‑1 (MQ=255) acTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGCACCa > 1:24020/1‑143 (MQ=255) cggCCTCCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGTACCATCTGa < 1:234353/142‑1 (MQ=255) gCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACTCCGGAAACaccaggcg > 1:606923/1‑136 (MQ=255) gCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGAcc > 1:285788/1‑143 (MQ=255) gCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTGGGCGCCAGTACCGGCCAAGAGAAAACGACGTTATCGAGCGCCGGCACGAGCTGAcc > 2:185201/1‑143 (MQ=255) cTTATCCTGGAGGAGGCCTTGCTATTAGTTAAAAAAAAGCACCTTAATGATGTGATATACGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAACGGACAAGAGACAACAACTTCATGGAACGCAGGAACCATCGAACCCt > 2:185696/1‑143 (MQ=255) tctcCTCGAGAAGGCCTTGCTGTTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTgg < 2:360292/143‑1 (MQ=255) cTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGa > 1:642183/1‑143 (MQ=255) tGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa < 1:508124/143‑1 (MQ=255) | AGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAA > NC_000913/3959384‑3959657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |