| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,376,822 | A→T | 13.3% | intergenic (+123/‑53) | ecnB → / → gdx | bacteriolytic entericidin B lipoprotein/guanidinium exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,376,822 | 0 | A | T | 13.3% | 61.9 / 2.3 | 30 | intergenic (+123/‑53) | ecnB/gdx | bacteriolytic entericidin B lipoprotein/guanidinium exporter |
| Reads supporting (aligned to +/- strand): ref base A (12/13); new base T (2/2); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
AACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTT > NC_000913/4376681‑4376960 | acccaaaGCGCAGCAATATGCAAGCACGGTACGACAGCGGTGTCGTGCCGTTTGGTTTTTCTGCGATAGTCACAAAGGTATTAGTTGAAATGCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGgacgac < 2:210255/139‑1 (MQ=255) gAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAATTATCCGTTCAACCATCAGCATTGCCGGACGACATg > 2:119887/1‑143 (MQ=255) acatcgcAGCAATAATCTAGAACGGTACGACAGCGGTTTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAACGTAATAGTCGAAATTCCCCTGCCACGTGGCAAAATATCCCTTCAACCATCAGCTTTGCAGGACGACCTGc < 2:114796/139‑1 (MQ=255) gcgcAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCaac > 1:206832/1‑142 (MQ=255) agcagcaATAGGCAATAACGGTACGACAGCTGTGTCGTGCCGTTGGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAATCATCAGCTTTGCAGGTCGTCCTGCaaag < 2:369239/142‑2 (MQ=255) agcaataagcaataACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAACAGTTGGAATTCCCCTGCCACCTGTCATAATCTCCGTTCATCCATCAGCTATGCCGTACCGCATGCAAACGcg > 2:577175/1‑142 (MQ=255) agcaataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTc > 1:565824/1‑143 (MQ=255) ataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCg < 1:197600/143‑1 (MQ=255) agaaCGGTACGAGAGTGCGGTCGTGTCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTGCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCg < 2:773305/141‑1 (MQ=255) gTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCGCTTTTCACCGGGGGCg > 2:599882/1‑143 (MQ=255) gACAGCTGTGTCGTGCCGTTTGTTTTTTCGTCGATAGACAAAAAGGTAATAGTGGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGccc < 1:700439/143‑1 (MQ=255) tgtgTCGTGGCGTTTGTTTTGTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATtc < 2:9648/143‑1 (MQ=255) tcccGTGTGTTTTGCCTGCGATAGTCACAAAGGTAATAGTCGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCACCCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAg < 2:336882/141‑1 (MQ=255) gCCGTTTGTTTTTTCTTCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGTCCTGCAAACGCCTCTTTTCTCCGGGGACGGCCCCAATTCTCCGGAGc < 2:279662/143‑1 (MQ=255) gtttgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTg > 1:181889/1‑143 (MQ=255) ttgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGat < 1:59366/143‑1 (MQ=255) ttgtttTTTCCGCGATAGTCACACAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGat < 1:59364/143‑1 (MQ=255) tGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGa < 1:730244/143‑1 (MQ=255) cGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCAGCGGGGACGGCCCCGATTCTCCGGAGCCGGATATGTCCGGGCtt > 2:209022/1‑143 (MQ=255) tCACAAAGGTAATAGTTGACATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTa < 2:569705/143‑1 (MQ=255) acaAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGt > 2:447817/1‑143 (MQ=255) aaGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAGCGCCTCGTTTCGCCGGGGGCGGCAATAATGCTCCGGAGCAAGATAGCTCCTGGGTTATGTGAGTTAt < 2:17723/143‑1 (MQ=255) gAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATGTGTCCTGGATTATCTTAGTTATTGCACGTctgcc > 2:244460/1‑142 (MQ=255) ggCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGt > 1:100212/1‑143 (MQ=255) aaaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCCATTCTCCGGAGCCTGAAATGTCATGAATTATCTTAGTTACTGCCCGTCTGCAGGCAATGGTATTGGCCGTTgg > 2:159567/1‑143 (MQ=255) atCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGa < 2:100212/143‑1 (MQ=255) cAACCACCAGCTGTGCAGGCCGACCTGCAACCGCCGCCTTTCACCGGCGACGGCCCCAATTCTCCGGAGCCCGATATGTCCTGGATTAGCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATAc < 1:36654/143‑1 (MQ=255) cATGAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCAc > 1:772125/1‑143 (MQ=255) tCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACgg > 1:734313/1‑143 (MQ=255) cgtttGCAGGTCGTCCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGCGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCtt > 2:389923/3‑143 (MQ=255) tttGCAGGTCGTCCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGTTATGTACTGGAGTGGCCTAGTTATTGCTGGTCTGCTGGGAGTGGTATGGGCCGGTGGCCTGAAATATACCCCCggggtta > 2:685361/1‑138 (MQ=255) gacgacCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGttt < 1:70436/143‑1 (MQ=255) | AACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTT > NC_000913/4376681‑4376960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |