| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 449,135 | C→A | 100% | V503L (GTA→TTA) | cyoB ← | cytochrome bo3 ubiquinol oxidase subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 449,135 | 0 | C | A | 100.0% | 82.6 / NA | 30 | V503L (GTA→TTA) | cyoB | cytochrome bo3 ubiquinol oxidase subunit 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (12/18); total (12/18) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAAC > NC_000913/448998‑449268 | cGGAGGCGGGGAAGAGGTGGCCCACTCCAGCGGACGGCCACCCCCCGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCAc < 1:293069/143‑1 (MQ=255) gAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGc > 1:212628/1‑143 (MQ=255) ccctcaggCGTGCGGCCCCCCCACGGCTCGCCAGTCGGGTCAGGGTTTTGTGCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCAt < 2:377741/136‑1 (MQ=255) tCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAGACGTACATCTGAATAACGAGGCCGAGAATACCCAGCGCCATCCGTAATGCACCGCTGCCCGCAAACCACAGCacgct > 2:71057/1‑139 (MQ=255) cAGCGTACGGCCACCCCCCTGGGCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGgtgt < 2:241921/143‑1 (MQ=255) aGCGTGCCGCCCCCCCACGGGGCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGtgtg < 2:564395/143‑1 (MQ=255) ggCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCCTGGTGTGGAACTGc > 1:645795/1‑143 (MQ=255) ggCCACCCCACGGGTCGCCAGTCAGATCACGGTTATGGTCGCGGTCGCGAATAGAAACGTACATCGGACTAACGAGGCAGAGGATAACCAGCGCAATAAGTAATGCACCGCTGGCTGCAATCAACAGCATGGGATGGAACAGc > 2:28877/1‑143 (MQ=255) cccccccTCGGGTCGCCAGGCAGGTCAGGGTTCTGGGCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAAGCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCgg < 2:180423/140‑1 (MQ=255) gTCGCCAGGCCGGCCACGGGTCTGGTCGCGGTCGCGAATAGAACCGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATctgct < 2:481908/143‑1 (MQ=255) gcGCCAGTCAGGTCCCGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATctgctg < 2:81465/142‑1 (MQ=255) gCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCaa < 2:83978/134‑1 (MQ=255) gCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCaa > 1:83978/1‑134 (MQ=255) cAGTCAGGTCACGGTTCCGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTc < 1:252169/143‑1 (MQ=255) cACGGTTCTGGTCGCGGTGCCCACTAGGAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACgg < 2:703955/143‑1 (MQ=255) gggcgcgAAGAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCTCCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAg < 2:633154/140‑1 (MQ=255) gcgAAGAGAGACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCa < 2:435737/143‑1 (MQ=255) aaaCGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATAc > 2:448650/1‑143 (MQ=255) gcGTACACCTGAGCACCGAGGCAGGGAACACCCGGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCTTACAg < 2:393029/142‑1 (MQ=255) aCGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGACGCCCAGCGCATACAg > 1:275765/1‑143 (MQ=255) ccaCATCTCAATAACGGGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTg < 1:41217/141‑1 (MQ=255) gAATAACGAGGGAGAGAATACCCCGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAg < 1:286062/143‑1 (MQ=255) aaTAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAgg > 1:283405/1‑143 (MQ=255) aaCGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGACGCCCAGCGCATACAGAGGCAGCAAGGGaa > 2:483447/1‑143 (MQ=255) aaCGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCaa < 2:652202/143‑1 (MQ=255) aaCGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCaa < 2:212628/143‑1 (MQ=255) gCAGAGAATACCCAGCGCACTCAGTAATGCACCGCTGGCTGCAACCATCAGCATCGTGTAGAACTGCGGCTCAATCTGCTGGCTCAGACGGCGGGCCATGCCCATGAAGCCCAGCGCACACGGTGGCATAAAGGcgacgacga > 2:402868/1‑143 (MQ=255) gCAGAGAATACCCAGCGCAATCAGCAATGCACCGCAGGCTGCAAGCCTCAGCATGGGGTGGAACTGCGGGGCAGACCGTCGGCTCGGCCGACGGGGCGAGCCCATGAGGCCCGGCCGACGCAGTGGCAGAGAGGCagagacgg > 2:634248/1‑136 (MQ=255) aGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATcca > 1:199666/1‑143 (MQ=255) cacccAGTAATGCACCGCTGTCTGCAATCATCACCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGCCGGGTCACGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATccagaac < 1:200380/139‑1 (MQ=255) | CGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAAC > NC_000913/448998‑449268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |