Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,439,845 T→A 11.5% intergenic (+52/+27) ytfK → / ← ytfL DUF1107 domain‑containing protein YtfK/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,439,8450TA11.5% 94.1 / 6.1 42intergenic (+52/+27)ytfK/ytfLDUF1107 domain‑containing protein YtfK/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (18/19);  new base A (3/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.36e-01

TTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATA  >  NC_000913/4439705‑4439983
                                                                                                                                            |                                                                                                                                          
ttaactcAAAATGGAATGTTAAGCCGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTc                                                                                                                                          <  2:611858/137‑1 (MQ=255)
    cccAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGa                                                                                                                                      >  1:220971/1‑143 (MQ=255)
       aaaaGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGcc                                                                                                                                   <  1:374092/143‑1 (MQ=255)
       aaaaGAGAGGGATAAGCGGCATGTGTCAGTGATGGCCGAAGGTAACCGTCAGGTTATGCGTCTGCAAACAGAGAGGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGcc                                                                                                                                   <  1:82677/143‑1 (MQ=255)
                       cTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGAGGTTTCCgggg                                                                                                                   >  2:397640/1‑143 (MQ=255)
                       cTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCCGATTCAGGAGCCGTTGATGTTTCTgggg                                                                                                                   >  2:299589/1‑143 (MQ=255)
                           aTTTGTCAGTGATGTCCGAAGGTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTa                                                                                                               <  2:171574/143‑1 (MQ=255)
                               gTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGAACGCTCCTGAATAAGGAGCCGTCGGTGCATCTGGGGGCTAcgcg                                                                                                           >  2:465321/1‑143 (MQ=255)
                                tCAGTGATGTCCGAAGTTAACAGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAAACAAAGTGCTATGGAGTACAAAGTGCTAAGCAGTAATAAGACGGCAACTGATTGAGGAGCCGTTGATGTTTCTGGGGGTTACGCGa                                                                                                          >  2:640020/1‑143 (MQ=255)
                                      aTGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCttt                                                                                                    <  2:775269/143‑1 (MQ=255)
                                            gAATTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCCTACACACAGCCCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTcttctt                                                                                              <  1:780304/143‑1 (MQ=255)
                                                   aacGTCAGGTTATGCGTCTGGAAAAAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttatcttt                                                                                       <  2:336688/141‑1 (MQ=255)
                                                      gTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGc                                                                                    >  1:393016/1‑143 (MQ=255)
                                                      gTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGAAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGATTCTGGGGGTTACGCGACGCTTTCTTCCTTATCTTTAGc                                                                                    >  2:285098/1‑143 (MQ=255)
                                                       tCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTCCTTCTTTATCTTTAGCg                                                                                   >  1:388572/1‑143 (MQ=255)
                                                          ggTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCa                                                                                <  1:428442/143‑1 (MQ=255)
                                                           gTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAg                                                                               <  1:675919/143‑1 (MQ=255)
                                                                 cGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGCTGTTTCTGGGGGTTACGCGACGATTTCTTCTTTATCTTTAGCGTCAGGCAGtt                                                                         >  2:49392/1‑143 (MQ=255)
                                                                  gTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGttt                                                                        >  1:240733/1‑143 (MQ=255)
                                                                  gTCTGCAAACAGAGATGGCTTAACCAAAGAGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATATTTAGCGTCAGGCAgagt                                                                        >  2:478340/1‑140 (MQ=255)
                                                                     tGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTgg                                                                     <  2:626788/143‑1 (MQ=255)
                                                                              agaTGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATAAAGGAGCCGTGGAAGGTTCTGGGGGTTACGCGACGCGTGCTTGTTTATCTTTAGCGTCAGGCAGTTTGAGCGAAAggcc                                                            >  2:484638/1‑141 (MQ=255)
                                                                               gaTGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCg                                                           <  1:578281/143‑1 (MQ=255)
                                                                                  ggCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTg                                                        <  2:220971/143‑1 (MQ=255)
                                                                                     ttAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGcc                                                     >  1:372574/1‑143 (MQ=255)
                                                                                      gaaCCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGTGCCGTTGATGTTTCTGGGGGTTACGCGACGTTTTCTTCTTTATCTTTAGTGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCt                                                    <  2:412790/142‑1 (MQ=255)
                                                                                        gccAGAGCGCGATGCCGTAAAAAGTGCTATGGAGTAATAGGCCGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCCTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTg                                                  <  2:431355/142‑1 (MQ=255)
                                                                                          cAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGTCTTGCt                                                <  1:65828/143‑1 (MQ=255)
                                                                                           aaagtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGGCGCTTTCTTCTCTATCTTTAGCGTCAGGCAGTCTTGGCGAAGGGGCGGTGGCCTCGCTg                                               >  2:189233/1‑143 (MQ=255)
                                                                                                  tatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATcc                                        >  2:668931/1‑143 (MQ=255)
                                                                                                       agtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTc                                   <  2:182898/143‑1 (MQ=255)
                                                                                                         taaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCAc                                 >  1:24627/1‑143 (MQ=255)
                                                                                                         taaaaagtgctacgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCAc                                 <  1:747069/143‑1 (MQ=255)
                                                                                                             atgtgctatgcagtaaTAAGACGGCTCCTGATTCAGGGGCCGTTTAGGTTGCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACcagc                             <  2:164028/141‑1 (MQ=255)
                                                                                                                   tatgcagtaaTAAGTCGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTgg                       <  1:232039/143‑1 (MQ=255)
                                                                                                                   tatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTCTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTgg                       >  1:232942/1‑143 (MQ=255)
                                                                                                                     tgcagtaaTAAGACGGCTCCTGATTCAGGTGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTc                     <  1:240285/143‑1 (MQ=255)
                                                                                                                                gACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGCTCCGGGTCACCAGCAGCCGGTCGGTGCGGTAGt          >  2:397385/1‑143 (MQ=255)
                                                                                                                                 aCGGCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTCTCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGCTCCGGGTCACCAGCAGCTGGTCGATGCGGTAGtt         >  1:325260/1‑143 (MQ=255)
                                                                                                                                 aCGGCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGtt         >  1:332056/1‑143 (MQ=255)
                                                                                                                                       ccTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTCGGCGAAAGGGCGGTGGCCTTGATGTCCTGCCGGGTACCAAGCAGCTGGGCGATGCGGTAGTGATCGat   >  2:467415/1‑143 (MQ=255)
                                                                                                                                        cTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGata  <  2:624820/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATA  >  NC_000913/4439705‑4439983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑