| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 454,891 | G→T | 100% | intergenic (+102/‑242) | bolA → / → tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 454,891 | 0 | G | T | 95.8% | 97.0 / ‑3.5 | 43 | intergenic (+102/‑242) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (20/18); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAC > NC_000913/454753‑455028 | ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCa > 2:783995/1‑143 (MQ=255) cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATT‑CAGACGaa < 1:421344/143‑1 (MQ=255) ggAAGCAGCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCg < 1:768983/143‑1 (MQ=255) cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGACAATTGCCCGGGAATGGTGa > 1:754777/1‑143 (MQ=255) aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcg > 2:64566/1‑143 (MQ=255) tCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCCTT‑CAGACGAAAATTGCCCGGGAGTTGCGAAAAAATTCGCGCCCgcgcccca > 1:31796/1‑139 (MQ=255) cgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc < 2:707428/143‑1 (MQ=255) gcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGCCAGCGCGCAATCAcc > 1:591844/1‑143 (MQ=255) gcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAcc > 1:681161/1‑143 (MQ=255) gcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGGCACGATGCTTCTTTATTTTGAGGGTTCTGCCCAGCATT‑CAGACGAAAATTGCCGGGGAATTGTGAAAAAATACGCGACAGCGCGGAATCAcc > 1:182446/1‑143 (MQ=255) tcAGTATGTTGTTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCGGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc < 1:246588/142‑1 (MQ=255) gcAGTGTGTTGCTAAATATTTTATTAAAACCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTGTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc < 2:74862/142‑1 (MQ=255) ccAGTATGTTGCTAAAGATTTTATGAAATCCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc < 1:431547/143‑1 (MQ=255) cAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctgg > 1:373506/1‑141 (MQ=255) gTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAACTTGCCCGGGAATTGTCAAAAAATACCCCACAGCGCCGAACAACCTTtctctgcacat > 2:312518/1‑136 (MQ=255) tGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaa < 1:768752/143‑1 (MQ=255) cTAAAGATTTTATGAAAAACGGCCTGCGTGCCGTTTTGATGGATCTTGATTTTGCGCTTTTTTCCCAGCATT‑CAGGCGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa < 2:346068/143‑1 (MQ=255) tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTTTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAAGTGCCCGGGTATTGTGAAAAAATACGAGACAGAGGGGAATAACCGTTGTCGAATCAGaaagg > 2:115084/1‑141 (MQ=255) tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTGCCAGCGTT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATCACCGTTCTCGCCTCATAACAg > 2:382304/1‑143 (MQ=255) tAAAGATTCTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGCTTTTGCGCTTTTTTCCCAGCATT‑CCGACAAAAATTGCCCGGGCATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAg < 1:257534/143‑1 (MQ=255) aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAACATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt > 2:483514/1‑143 (MQ=255) aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt > 1:332898/1‑143 (MQ=255) aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATCTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt < 1:68412/143‑1 (MQ=255) agagTTTATGAATAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa < 2:211854/143‑1 (MQ=255) tttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc > 1:644494/1‑143 (MQ=255) tGAAAACCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTCCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta < 1:97391/143‑1 (MQ=255) gAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGGGGTTTTTTCCGAGCATT‑CAGACGAAAATTGCGGGGGAATGGTGAAAGAATACGCGACAGCGCGCAGTAACCGGTCTGGACTCATAAAAGTGATGGCGCtat > 1:389492/1‑143 (MQ=255) cGGCCTGCGGGCCGAGGTGGTGTGTCTGGATGTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc < 1:10350/143‑1 (MQ=255) cGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAATTTGCCCGGGAATTGTGAAAAACTACGCGACAGCGCGCCATAACCGTTCTCGACTCATAACAGTGATGCCGCCATAATGCCGCGTCCt > 2:481279/1‑143 (MQ=255) ggCCGTTGTGTTGTGTCTGGATTTTGCGTTTTTGTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAGAATACGCGACAGCGCGCAATAACCGTGCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAt < 2:269909/143‑1 (MQ=255) gCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTCCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGCCTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTCtt > 2:266398/1‑143 (MQ=255) ttttgttttgTCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa > 1:260572/1‑143 (MQ=255) gtttggtttgtCCGTATTTTGCGCTTTTTTCCCATCATT‑CGGACGAAATTTGCCCGGGTATTGTGAAAAAATATGCGACTGCGCGCACTACCCGTTCTCGACTCATAAAAGTGATGCTTCTATAATGCCGCGTCCTATTTGaa < 2:318282/142‑1 (MQ=255) tgtCTGGATTTTGCGCTTTTTTCCCAGCATT‑CACACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACCCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCg < 2:23077/143‑1 (MQ=255) gtCTGGATTTGGCGTTTGTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAAAAACGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg < 1:403047/143‑1 (MQ=255) tCTGGATTTTGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTCTTTGAATGCTTTCtgg > 1:434730/1‑143 (MQ=255) cTGGATTGTGGGCGTTTGGCCGAGCATT‑CAGTCGAAAATTGCCCGGGCATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga < 2:273980/143‑1 (MQ=255) ttttGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt > 1:476584/1‑143 (MQ=255) tGCGCTTTTTTCCCAGCATT‑CATACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATATCCGTTCTCGACTCTTAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg < 2:787980/143‑1 (MQ=255) tGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg < 1:783995/143‑1 (MQ=255) tGCGCTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg < 1:202834/143‑1 (MQ=255) tGCGCTTTTT‑CCCAGCCTTCCAGCCGAAAATGGCCCGGAATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGACATCATTCTg < 2:733825/143‑1 (MQ=255) gcgcTTTTTTCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCCTAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGCTGGTTCTgg > 1:130963/1‑143 (MQ=255) ttttttCCTAGCCTT‑CAGACTAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCGCCACTCATAAAAGTGATGCCGCTATAATGCCGCGTACTATTTGAATGCTTTCGGGATGATTCTGGTAAc < 2:725789/143‑1 (MQ=255) | CTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATT‑CAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAC > NC_000913/454753‑455028 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |