| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 594,950 | T→G | 11.2% | H165P (CAT→CCT) | cusR ← | DNA‑binding transcriptional activator CusR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 594,950 | 0 | T | G | 11.2% | 67.4 / 2.6 | 39 | H165P (CAT→CCT) | cusR | DNA‑binding transcriptional activator CusR |
| Reads supporting (aligned to +/- strand): ref base T (20/13); new base G (2/3); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01 | |||||||||||
GAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCC > NC_000913/594810‑595089 | gAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCACATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATgg > 1:172105/1‑143 (MQ=255) ttAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTGCGCCCTGATGGCga > 1:582160/1‑143 (MQ=255) gCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCTCCGCCGCTTCACTGCTACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaagt < 2:514662/143‑2 (MQ=255) gCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaagt < 2:339806/143‑2 (MQ=255) gCTGCGGCGCAAAGTCGGTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaagt < 1:340133/143‑2 (MQ=255) tCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCCACATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCCATTCGCCCTGACGGCgcaggagg > 2:526559/1‑141 (MQ=255) tCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAACCGAGCGGGGAAGCACTTCGCCCGACTGGCgaaggaag > 2:511894/1‑143 (MQ=255) tCGGCTCAAAGTCGTTGCCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCCATAGCATTGGTATAACCGCCAAACTTGATGTCCCATACCTGCGAGGCGAGAAGCGAGCGGGGCAGCACTTCGCGCTGCGGGGgaaggggg > 2:237390/1‑140 (MQ=255) ggCTCAAAGTCTTGTTCGGTTTTGCCGCGCCGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGAGAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGgaagaa < 2:229346/143‑1 (MQ=255) acagccGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACAGCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCGGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCca < 2:352931/138‑1 (MQ=255) gTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCcagcagcgcac > 2:136558/1‑138 (MQ=255) tCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATAGCGACCGAAGAACTCCAGCCGAGTaag > 2:86432/1‑142 (MQ=255) tCGATTTTGCCGCGCAGCCGCATCACCGCCACATCAATAGCATTGGTATCGCTGCCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACGTCGCCCTGATGGCGCAGGAAGAACTACAGCagagacaa > 2:272587/1‑139 (MQ=255) tCGATTTTGCCGCGCAGCCACTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGCTAAGCGAGCGGGGCCGCACTTCGCCCTGATGGCGAAGGGAGCACTCCAGCCGAGTcaa > 2:111447/1‑143 (MQ=255) cGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGGCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAAc > 2:132120/1‑143 (MQ=255) aatttGCCGCGCAGCCGCTTCACCGCCACCTCAATAGCATTGGTATCGCTGTCCAACTTCATGTCCCATACCTGCGAGGCGATCAGCGAGCGGGGCAGCACTTCGCCCGGATGGCGAAGGACGCCCCCCCGCAGAGCAAAccc > 2:231789/3‑141 (MQ=255) ttttGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATACGAATCGCTGTCAAACTTCAAGTCCCATAACTGCGAGGCGATAAGCGAGCGGGGCAGCACCTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTACActat > 1:166551/1‑141 (MQ=255) gCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGCGGCGATAAGCGCGCGGGGCAGCCCTTCGCCCTGATGGCGACGGAAGAACTCCAGCAGCGCAAACTCTTTAc > 1:286539/1‑143 (MQ=255) gCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCGCTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGGAAACTCTTTAc > 1:136502/1‑143 (MQ=255) ccGCGCAGCCGCTTCACTGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAAGCGATAAGCGAGCGGGCGAGCACCTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACt > 2:638217/1‑143 (MQ=255) gCCGCTTCCCCGCCAAATCAATCGCAGTGGTACCGCTGTCAAAATTGATGTTCCAGACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAATGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCaaa < 1:70855/143‑1 (MQ=255) ccGCTTCAACGCCCCAACAACAGCCTTGGTCTCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTTATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAg < 1:752334/143‑1 (MQ=255) ccACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTg < 1:721930/143‑1 (MQ=255) tagaagtGGTATCGCTGTCAAAATTCATGTCCCATGCCGGGGAGGCGATAAGCGAGCGGGGCGGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCgg < 1:332837/137‑1 (MQ=255) tAGCATTGGTGTCGCTGCCCCCCTCCATGTTCCATACCTGCGAGGCGATAGGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCgg < 2:374833/143‑1 (MQ=255) actactATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGa < 1:503541/138‑1 (MQ=255) aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCCGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGGGCCGCTGCGGGTGa > 1:646929/1‑143 (MQ=255) tATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTg > 1:713389/1‑143 (MQ=255) tCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGCACTCCAGCATAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGAc > 1:575278/1‑143 (MQ=255) aaTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTGGTCTTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACgaga < 1:443598/143‑1 (MQ=255) aaTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCCCCCTGAGGGCGAGGGAAGAACTCCAGCAGAGTAAACCCTTACCCGGCCAAGGTGAGCCGCGTGCCGCCCCGGGTGCCTTGGCGCCTGACgaga > 2:126495/1‑143 (MQ=255) aTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACCCTTTAACGGTCAAAGTGATGCGCGTGCCGCCGCGGGTGACTTTGCGGCTGACGACAt > 1:292578/1‑143 (MQ=255) gCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAGCTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACGATCACATCGGCGACCTg > 1:752696/1‑143 (MQ=255) aTAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGaaa < 1:638027/143‑1 (MQ=255) gggCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGGGACTGACttgtgc > 1:47783/1‑139 (MQ=255) agcaCGTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAAt < 1:725667/143‑1 (MQ=255) gggggtggCCTTGGTGGGGAAGGAGGGGCTCCAGCAGAGTAAACGCGTTACTGGTCAAAGTGATGCGCGTGCCGTTGCGGGGGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATc < 2:335871/136‑1 (MQ=255) cTTCGACCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACGCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACGTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCAcc < 1:415799/143‑1 (MQ=255) tGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGAACGACCATCGAATCGGCAACCTGAAACTGACTGTCGATAAACAACGCCGcccc > 2:556350/1‑143 (MQ=255) | GAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCC > NC_000913/594810‑595089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |