Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,368,101	T→C	A21A (GCT→GCC)	arnA →	fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,368,101	0	T	C	100.0%	60.2 / NA	25	A21A (GCT→GCC)	arnA	fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/9);  total (16/9)

GATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTG  >  NC_000913/2367961‑2368236                                                                                                                                             |                                                                                                                                        gATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGGCAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTGTTGACGCCCCGCTTGCCGc                                                                                                                                       >  1:614050/1‑143 (MQ=255)     tACACCGGTGTCCGCGGCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGt                                                                                                                                   <  2:330063/143‑1 (MQ=255)      acacCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATTATTTATGTTATCCGTCGTTTTTGCCTACCAAGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGtt                                                                                                                                  <  1:511387/143‑1 (MQ=255)       cacCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTa                                                                                                                                 <  1:715110/143‑1 (MQ=255)        acCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGttgc                                                                                                                                >  1:931965/1‑141 (MQ=255)          cGATGACCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCAACCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGAAGCCACAGTAATGAAGCCCTGCTGGCCGCAGCTTACGa                                                                                                                              >  2:887028/1‑143 (MQ=255)                ccGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTTGTTTTTGTTTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAg                                                                                                                        <  1:767262/143‑1 (MQ=255)                           cccGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGCTACGAAATCAGCGCCACCTTTa                                                                                                             >  2:731312/1‑143 (MQ=255)                           cccGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGATTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGACATTAGCGCCATTTTTa                                                                                                             >  2:111108/1‑143 (MQ=255)                                 aTTGTGGTCGGCAGGTTAGCCCTGCATCGAGCAAGGAAAATGAATAATGAAAACCGTCGTGTTTGCCTGCCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATa                                                                                                       <  2:38879/143‑1 (MQ=255)                                     tgtttcgCAAGTTCTCTGGACAGCCAGCAAGGAAAATGAATAATGAAAACCGTCGGTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGa                                                                                                   <  2:977067/137‑1 (MQ=255)                                     tGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTCCGAACTTACCGCCATTTTTACCCATCCTGa                                                                                                   >  1:598149/1‑143 (MQ=255)                                                         catccaGCAAGGAAAATGAATAATGAAAACCGTCGTTGTTTTTTTTTTTGTTATCGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCTGGTTACGAAGTTAGCGCCATTTTTACCCATACTCATCATCCCGTAGAAAAAGCCt                                                                               >  2:649524/1‑143 (MQ=255)                                                               gCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGTTAGTCTCGGTGAAAATGCCTtataag                                                                         >  1:479111/1‑141 (MQ=255)                                                                cAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGTGGCGGGTTTACGCATTTTGTTGTGCCCGGTTAAAATGTCTTTTATgt                                                                        >  1:774442/1‑142 (MQ=255)                                                                              aatGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATGTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTc                                                          <  2:507985/143‑1 (MQ=255)                                                                                        gtcgtTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGACATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTATAGCTGCGGa                                                >  2:881142/1‑143 (MQ=255)                                                                                               ttGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTCTGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGc                                         >  1:187164/1‑143 (MQ=255)                                                                                                    tACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTcg                                    >  1:593771/1‑142 (MQ=255)                                                                                                     aCCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTATCCATATTGATTTTCTCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCg                                   <  1:319278/143‑1 (MQ=255)                                                                                                                ggATGCCTGGGTATTGAAGCCCGGCTGGCCGCCGGTTACGAAATTAGCGCGATTTTTCCCCATACTGATAGTACTGTTATAAAATTCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGcc                        <  1:957843/143‑1 (MQ=255)                                                                                                                      cTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTTAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGCAAGAGGCATTCCGGATTATGCGCCGGGTaa                  >  2:14578/1‑143 (MQ=255)                                                                                                                          gTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCAGGCTTATGCGCCGGATGACGtt              >  2:850130/1‑143 (MQ=255)                                                                                                                               gAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGGAAGCGGCATTCCGGATTATGCGCAGGATAACGTTAatca         >  1:221182/1‑143 (MQ=255)                                                                                                                                      tgctgGCCGACGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGCAAGAGGACTTCCGGTCTATGCGCCGGATAGCGTTAATCGTCCGCtg  >  2:1050278/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                        GATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTG  >  NC_000913/2367961‑2368236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑