Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	454,891	G→T	intergenic (+102/‑242)	bolA → / → tig	DNA‑binding transcriptional dual regulator BolA/trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	454,891	0	G	T	100.0%	173.9 / NA	57	intergenic (+102/‑242)	bolA/tig	DNA‑binding transcriptional dual regulator BolA/trigger factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/28);  total (29/28)

GCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAC  >  NC_000913/454751‑455028                                                                                                                                             |                                                                                                                                          gCCTCTCCTCCCGTTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTcc                                                                                                                                         <  2:985161/143‑1 (MQ=255)   gtctcggcccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCa                                                                                                                                       <  2:339928/136‑1 (MQ=255)     ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTCTGAGAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGc                                                                                                                                     >  1:491330/1‑143 (MQ=255)       cctccGTGTCGTGGAGCAGGGAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAGACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCAt                                                                                                                                   <  2:375669/143‑1 (MQ=255)          cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGCTTTTGCGCTTTTTTCGCAGCATTCa                                                                                                                                >  1:441295/1‑143 (MQ=255)           ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCAGGATTTTGCGCTTTTTTCCCAGCATTCAg                                                                                                                               >  1:682311/1‑143 (MQ=255)           ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACAGTATGTTGCGAAAGATTGTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCGGGATTTTGCGCTTTTTTCCCAGCGTTCAg                                                                                                                               >  1:515614/1‑143 (MQ=255)                cGTGGGGCAGGAGGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATCTTCTGAATATCGGCCTGTGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGaa                                                                                                                          <  2:227094/143‑1 (MQ=255)                     ggCAGGAAGTGTCGCGTAAAACCGCATGTGCAGCTGTCGGCGCTTTGCCAGGATGTTGCTAAAGATTTTATGAAATACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTg                                                                                                                     <  2:496498/142‑1 (MQ=255)                      gCAGGAAGCATCGCGTAAAAACGCATTTGCAAGTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGc                                                                                                                    <  2:847511/143‑1 (MQ=255)                          gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTGCCGGTCTGTTGCTCAAGATGTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCgg                                                                                                                <  1:1060951/143‑1 (MQ=255)                          gAAGCATCGCATAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCgg                                                                                                                >  2:940672/1‑143 (MQ=255)                               aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAAtt                                                                                                           >  2:509234/1‑143 (MQ=255)                                  gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATtgtg                                                                                                        <  2:813941/143‑1 (MQ=255)                                    gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGTTTTTGCGCTTTTTTCCCAGCATTTGGCTGAAAATTGAACGGGAAATGTGaa                                                                                                      >  1:18718/1‑143 (MQ=255)                                    gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaa                                                                                                      <  2:166441/143‑1 (MQ=255)                                         caCGCACTGGCAACTGGCGGCGCTGTCCAAGGATGTTGCTAAAGATTTTAGGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                                                                 <  2:192713/142‑1 (MQ=255)                                            gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCAGTCCGACGACACTTGCCAGGGCATTGTGAAAAAATAcc                                                                                              >  2:466094/1‑142 (MQ=255)                                            gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGAATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcg                                                                                              >  2:911395/1‑143 (MQ=255)                                             cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTTCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgc                                                                                             <  2:550486/143‑1 (MQ=255)                                                     aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgc                                                                                     <  1:940672/143‑1 (MQ=255)                                                               cTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGACAAAATACGAGACAGCGCGCAATCACCg                                                                           >  1:298351/1‑143 (MQ=255)                                                                     cAGTATGTTGCTAAAGATTTTATGAACAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCCATCACCGTTCACg                                                                     >  2:1020741/1‑143 (MQ=255)                                                                     cAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                                     >  2:911444/1‑143 (MQ=255)                                                                      ggTATGGTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                                    <  1:388083/142‑1 (MQ=255)                                                                      cgTATGTTGCTAAATATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                                    <  1:254406/142‑1 (MQ=255)                                                                      aGTATGTTGCTAAAGATTTTATGAAAACCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                                    <  1:573435/143‑1 (MQ=255)                                                                      aGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                                    <  1:31418/143‑1 (MQ=255)                                                                        tATGTTGCTAAAGTTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACt                                                                  <  2:102184/143‑1 (MQ=255)                                                                          tGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCa                                                                <  1:83985/143‑1 (MQ=255)                                                                           gTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAt                                                               <  2:583244/143‑1 (MQ=255)                                                                            ttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCCGCATTCAGACGAAGCTTGCCCGGGAATTGTGAAACAATACGCGCCAGCGCGCTATAACCGTTCCCGAccccta                                                              >  2:514708/1‑138 (MQ=255)                                                                               cTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaga                                                           >  1:942968/1‑141 (MQ=255)                                                                                tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTGCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaataa                                                          >  1:680592/1‑140 (MQ=255)                                                                                tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGGCTTATgaagg                                                          >  1:677979/1‑141 (MQ=255)                                                                                 aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCACTCACCGTTGTCGACTCATAAAAGt                                                         >  2:837029/1‑143 (MQ=255)                                                                                 aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTc                                                                 >  2:988910/1‑135 (MQ=255)                                                                                 aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTc                                                                 <  1:988910/135‑1 (MQ=255)                                                                                      ttttATGAAAAACGGCCTGCTGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                    <  2:176058/143‑1 (MQ=255)                                                                                           aacaaaaTGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta                                               >  2:40750/4‑143 (MQ=255)                                                                                            gAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCAGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAGCCGTTCTCGACTCATAAAAGTGATGCCGCtat                                              <  2:682311/143‑1 (MQ=255)                                                                                            gAAAAACGGCCTGCGGGCCGTTTTGTCTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGCTGCCGCtat                                              >  2:936729/1‑143 (MQ=255)                                                                                             aaaaaCGTCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCTTTCAGACGAAAATTGCCCGGGTATTGTGAAAAAATACGCGACAGCGCGCAATACCCGTTCTCGACTCATAAAAGTGATGCCGCtatc                                             >  2:458253/1‑142 (MQ=255)                                                                                             aaaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAACCCGCGACATCGCGCATTAAGCCATAGACACTCAAAAAAGTGATGCCGGtata                                             >  1:145434/1‑143 (MQ=255)                                                                                             aaaaaCGGCCTGCGGGCCGGTTTGTTTTGTCTGGATTTTGCGCTTCTTTCCCCGCATTCAGACGAACAGTGCACGGGAATTGTGAAAAAACACGCCACAGCGGGCAATAACAAGTCCCGACTCCGACAAGAGAAGCCGccacc                                             >  2:317218/1‑139 (MQ=255)                                                                                                 aCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATG‑CGCTATAATGcc                                        <  1:911444/143‑1 (MQ=255)                                                                                                  ctgCCTGCGGGTAGTTTTGTTTTGTCTGGATTTTGCTCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGAGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                                        <  1:572760/141‑1 (MQ=255)                                                                                                  cGGCCTGCGGGCCGTTTTGGTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGGCGGGGAATTGTGAAAAAATACGCGACAGCGCGCGATAACCGTGCTCGACTCATAAAGGTGATGCCGCTATAATGcc                                        >  1:765487/1‑143 (MQ=255)                                                                                                   ggCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                       <  1:245385/143‑1 (MQ=255)                                                                                                    gCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgc                                      >  2:338072/1‑143 (MQ=255)                                                                                                          gggCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTa                                >  2:55503/1‑143 (MQ=255)                                                                                                                 tttgttttgtCTGGATTGTGCGCTTTTTTCCCAGCATTCAGGCGAAAATTGCGCGGCAATTGTGCCAACAGAAGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAAt                         <  2:320432/143‑1 (MQ=255)                                                                                                                     ttttgtCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTCTAATGCCGCGTCCTATTTGCCTGCtt                     >  1:858112/1‑143 (MQ=255)                                                                                                                     ttttgtCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt                     >  1:1029571/1‑143 (MQ=255)                                                                                                                         gtCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg                 <  2:241002/143‑1 (MQ=255)                                                                                                                            tGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTACACTGCCGCGTCCTATTTGAATGCTTTggggtt              >  2:213369/1‑141 (MQ=255)                                                                                                                              gATTTTTTGCTTTTTTCCCAGAATGCAGAGGAAAATTGCACGGGAAGTGTGAAAAAATACGCGACAGCGCGCAAGAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatga            <  1:466094/143‑1 (MQ=255)                                                                                                                                        ttttttCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAc  >  1:318740/1‑143 (MQ=255)                                                                                                                                        ttttttCCAAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACAGCTCTCGACTCATAAAAGTGATGCCGCTATAATTCCGCGTCCTAGTTGAATGCTTTCGGGATGATTCTCGTAAc  >  1:40361/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                          GCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAC  >  NC_000913/454751‑455028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑