Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,446	A→G	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	150.3 / NA	52	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/25);  total (27/25)

GATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGG  >  NC_000913/754314‑754580                                                                                                                                     |                                                                                                                                       gATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCttt                                                                                                                              <  2:839381/143‑1 (MQ=255)  aTGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                             <  1:755666/143‑1 (MQ=255)     cGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACACCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTCGTa                                                                                                                          >  2:736866/1‑143 (MQ=255)        ttGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCCACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                       >  1:111352/1‑143 (MQ=255)        ttGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                       >  1:840928/1‑143 (MQ=255)             gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCCGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAt                                                                                                                  <  1:556960/143‑1 (MQ=255)             gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCACCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGCGGGTCGGTTTTGCTCTTGTATCAGCCAt                                                                                                                  >  2:252663/1‑143 (MQ=255)              tgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAtt                                                                                                                 >  1:614335/1‑143 (MQ=255)                 agCCTGGGTCAAAGCTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                              <  1:993622/143‑1 (MQ=255)                  ggcggggTCAAAGGTGAACAGACCTTTGGAGCCGAGCGTGGAAATAACAATAACATCTTGACCCAGCGTGCCGTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                             <  1:888540/139‑1 (MQ=255)                  gCCGGGGTCAAAGGGGAACACCCCTTTGGGACCGGGAGTACGGATATCAATAACAGCTTGACCCAGCGTGCCTGTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                             <  2:182128/143‑1 (MQ=255)                   ccTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGAGCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTGTGCTTTTGTATCAGACATTTACgg                                                                                                            >  1:277924/1‑143 (MQ=255)                        gTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTcc                                                                                                       <  2:574261/143‑1 (MQ=255)                          cAAAGGTGAACTCACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCtt                                                                                                     <  1:898777/143‑1 (MQ=255)                           aaaGCTGACCACACCTTTTCAACCGCGTGTTCGGATATCACTAACAGCGTGACCCAGCGTGCCTTTCAGCACATTCAGTCCTTCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTa                                                                                                    <  2:409644/143‑1 (MQ=255)                               gTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTGTCGTTAgcgc                                                                                                >  1:500308/1‑143 (MQ=255)                                    cacacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAt                                                                                           >  2:851127/1‑143 (MQ=255)                                     acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTGAGCGCCTTAtt                                                                                          >  1:983470/1‑143 (MQ=255)                                       tccTTTTGAACTGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGTGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                        <  1:820469/142‑1 (MQ=255)                                       acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCGTTAGCGCCTTATTGc                                                                                        >  1:486042/1‑143 (MQ=255)                                        ccTGTTGAAGCGAGCGTACGGATATCAATAGCATCTGTGCCCAGCGGGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTAGCAGCAAAATAAGTGTTTCTTAGCGTCTTATTGCg                                                                                       <  2:936177/143‑1 (MQ=255)                                          ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTa                                                                                     >  2:484041/1‑143 (MQ=255)                                          ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACACCAAGATCACAAGCTATCACCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCCTTTAAGGTCTCCTTAGCGCCTTACTGCGTa                                                                                     >  2:377443/1‑143 (MQ=255)                                             tGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGa                                                                                  <  1:926232/143‑1 (MQ=255)                                             tGAACCGAGAGTACGGATAGCAATAACATCTTTTCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTCAGa                                                                                  <  1:556406/143‑1 (MQ=255)                                                    agagTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCCTTTAGGGTCTCCTTCGCGCCTTATTGCGTAAGACTGACgg                                                                           >  2:88329/1‑143 (MQ=255)                                                     gagTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCCGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGcagaa                                                                          >  1:260856/1‑139 (MQ=255)                                                     gagTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGa                                                                          <  2:496971/143‑1 (MQ=255)                                                       gTACGGACATCAAGAACGTCTGGCCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTCTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAAc                                                                        <  1:900226/143‑1 (MQ=255)                                                        tACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACt                                                                       >  1:848718/1‑143 (MQ=255)                                                           ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaa                                                                    <  1:271211/143‑1 (MQ=255)                                                           ggAGATCAAGAACGTCTTGCCCCAGCGTGCCTTTCAGCACAGCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGGATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaa                                                                    <  1:111834/143‑1 (MQ=255)                                                                  aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGcc                                                             >  2:231371/1‑143 (MQ=255)                                                                   aTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTACCt                                                            >  2:541384/1‑143 (MQ=255)                                                                    tAACATCTTGACCCAGCGTGCCTTTCAGAACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAGTTGCCtg                                                           >  2:764698/1‑142 (MQ=255)                                                                              aCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCaa                                                 >  1:221549/1‑143 (MQ=255)                                                                                         cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAc                                      <  1:210695/143‑1 (MQ=255)                                                                                                  caTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt                             <  1:563962/143‑1 (MQ=255)                                                                                                   aTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAAGTTAAATTTGCCTTCGCACATCAACCTGGCTTTACTCGTTTTTTa                            >  1:508391/1‑143 (MQ=255)                                                                                                       aGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTg                        >  1:840976/1‑143 (MQ=255)                                                                                                           cAACAGCTGTATCCCCGTTGAGGGTTGGTTTTGCTTTTGTATCAGCCATTTATGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTc                    <  2:597238/143‑1 (MQ=255)                                                                                                           cAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTc                    >  2:60834/1‑143 (MQ=255)                                                                                                           cAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTGTTTATTTGGCTc                    >  1:165164/1‑143 (MQ=255)                                                                                                            aacgcgtgtgTCCCCGTTGTGTGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTCTTTGGCTcg                   <  2:1026649/137‑1 (MQ=255)                                                                                                                 cTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGACCCTACGACTCGCTTTTCCCGTTTTTTATTTGGCTCGCCGct              >  1:723360/1‑143 (MQ=255)                                                                                                                  tGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATCTAAGATCTCCTTAGCGCCTTATTGCGTAGGACTGCCGGAACTTACATTTGCCTTCGCCCATCCACCTGGCCTTGCTCCATTTTCATTTGGCTCgctgctg             >  2:873746/1‑142 (MQ=255)                                                                                                                     agccccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt          <  1:484041/141‑1 (MQ=255)                                                                                                                      tCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCGTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGGCGCTCtgtt         >  2:563065/1‑142 (MQ=255)                                                                                                                        cccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTAACTGGTGTTGTTTTTGACTTGCCGCTCggtcta       >  2:748076/1‑140 (MQ=255)                                                                                                                            ttGAGGGTTGGTTTTGCTTTTGTATCAGCCCTTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGGAtgag   <  2:712330/143‑1 (MQ=255)                                                                                                                            ttGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAagag   <  1:426886/143‑1 (MQ=255)                                                                                                                             ggAGGGTGGGTCTCGCTTTTGTATCAGCCATTTAAACTCAACACATCGCCGTATAGCAAACTAGTTCCGTAACTTAAATTTGCCTTCTCACAACAACTTGGCTTGACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg  <  1:145533/142‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGG  >  NC_000913/754314‑754580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑