| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 91.9% | 93.3 / NA | 37 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (13/21); total (15/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760361‑760621 | gcggcggCAGGAGCTGGCGAGACGCATCAGGACGGTGCCGGAAGCAGTTGAAATGCAGGCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCg < 2:485594/137‑1 (MQ=255) gcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCt < 1:71491/143‑1 (MQ=255) aGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACg > 1:896101/1‑143 (MQ=255) cTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCt < 1:669538/143‑1 (MQ=255) gCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCGAGATGGATGGCGATCGCCAGGCGAGGGCTGCCGGAGAGAGACGGCTCGACTGGGGGGGCGCGGAAGAACCCGCTTACGCCACGCTggg > 2:982137/1‑142 (MQ=255) cGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGtt < 2:492254/143‑1 (MQ=255) gAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTCCGCCACGCTGGTtg > 1:189358/1‑143 (MQ=255) aaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtggt > 1:927206/1‑141 (MQ=255) gCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGaa < 1:171627/143‑1 (MQ=255) gCATCAGCACGGTGCCGGAAACAGTTGAAATGCAGTCTCGCGTTGCCGGGAATTATGGCGGTCGGGAGGCGATGGCTGCCGGTGAGAAACTGTTGGACTGGGGCGGTGCGGAAAATATCGATTACGCCACGCTGGTTGATGaa > 2:572141/1‑143 (MQ=255) aTCAGCACGGTGCCGGAAGCAGTGGAAATGCAGTCTCGCGTTGCCAAGATTTAGGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAgg < 1:973941/143‑1 (MQ=255) tCAGCACGGTGCCGAAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGAACGCCAGGCGATGGCTGCCCGAAAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCACGCTTACGCCACGCAGGTTGATAAAGGc > 2:339299/1‑143 (MQ=255) gTGCCCGAAGCAGTTGAAATGCAGGCTCGCGTTGCCAAGATCTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGTCACGCTGGTTGATGAAGGCATTCCGGt < 1:856231/143‑1 (MQ=255) gCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGAGAGGCGATGGCTGCCGGTGAGAAACTGTTAGACTGGAGCGGTGCGGAAAACGTCGCTTACGCCAGGCTGGTTGGTGAAGGCATTCCGGttg > 2:543094/1‑142 (MQ=255) ccGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCg > 1:911914/1‑143 (MQ=255) cAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCg < 1:756107/143‑1 (MQ=255) agctGCGGTCTCGCGTGTCGAAGAGTGATGGCGTTCGCCTGGCGATGGCTGCCGGTGAGAAGCTGTTCGACTGGAGCCGTGCGTAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGaa < 2:159527/140‑1 (MQ=255) aaaTGCAGTCTCGCGTGGCCAAGATGTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAATCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGaa < 2:376580/143‑1 (MQ=255) ccgccgcgcgTTGCCAAGAGTGATGGCGATCGCCAGGCGGTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTc < 2:768222/137‑1 (MQ=255) gttgtctcgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTcc < 1:1067965/136‑1 (MQ=255) gTTGCCAAGATTGATGGCGCTCGCCAGGCGATGGCTGCCGGTGAACGATGGGGCGGCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCgg < 1:1012955/143‑1 (MQ=255) aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCTGAAAACCTCGTTTACGGTACGCTGGTTGATGAAGGCATTCCTGTTCGCCCGTCGGGTGAAGACTCCGGTCGCGGTACctt > 1:780380/1‑143 (MQ=255) aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCAGTCCGGATCGCCTCACGCGTGAAAACTCTGGTggcgatgactc > 2:711132/1‑136 (MQ=255) ttgaTGGCGATCGCGAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttc < 2:1047104/140‑1 (MQ=255) gggCGGTCGGGAGGCGATGGTTGCTCGTTAGAGCTGGTTCGGCTGGGGCGGTGCGGAATGCCCCGCTTACGCCACGCTTGTTGATGAAGGCATTCCGGTTCGCCGGTCGGGTGAAGTCTTCGGTCGCTGTACCTGCTTCCAcc < 2:654244/142‑1 (MQ=255) ggtggtCGCGAGGCGATGGCTGCTGGTGAGAAGCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTGCGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCg < 2:1067938/138‑1 (MQ=255) cGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTGCTTCCACCGcc > 1:447690/1‑143 (MQ=255) ccAGGCGATGGCTGCCGGTGAGAAACTGTTCGCCTGGAGCGAAGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCCTCTTCCACCGCCAcgcgc > 2:873088/1‑142 (MQ=255) cAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGt > 1:603282/1‑143 (MQ=255) ggCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGa < 1:1049104/143‑1 (MQ=255) gATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCCGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATcc < 1:688634/143‑1 (MQ=255) tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGGTGACGGCATTCCGCCGCGCCGGGCGGGTGAAGACTCCGCTCGCGGCACCTTCCTCCACCGCCCCCCGATCATCCACAAcc > 2:123578/1‑143 (MQ=255) gCCGGTGTTAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCa < 2:984076/143‑1 (MQ=255) aaaCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg < 2:269174/79‑1 (MQ=255) aaaCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg > 1:269174/1‑79 (MQ=255) gTTCGACTGGAGCGGTGCGGAGAAGCCCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCa < 1:912328/143‑1 (MQ=255) gACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTa < 1:42910/143‑1 (MQ=255) | GCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760361‑760621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |