| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,987,450:1 | +A | 8.0% | intergenic (+22/+57) | ftnB → / ← yecJ | putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,987,450 | 1 | . | A | 8.0% | 181.2 / 11.3 | 50 | intergenic (+22/+57) | ftnB/yecJ | putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ |
| Reads supporting (aligned to +/- strand): ref base . (23/23); new base A (2/2); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01 | |||||||||||
GATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGG > NC_000913/1987312‑1987592 | gatgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGACACGTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGC‑AATGCAttagcgc < 1:232388/143‑6 (MQ=255) tCTGTTGGTGCAAACCAGTCTTGATGAAGTGCGGAGTGCGATACTTGCGGGTATGTGCACTGTGAAGCCCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGC‑AATGCATTAGCGCCGATg < 2:53822/143‑1 (MQ=255) cggtggctgcAAACCATTCTTGATGTAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGC‑AATGCATTAGCGCCGATGa < 2:519271/138‑1 (MQ=255) tGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGaa < 2:490076/143‑1 (MQ=255) gTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGACGAAg > 2:516794/1‑143 (MQ=255) tgcAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGCAGGtttt > 1:733963/1‑143 (MQ=255) tgcAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGtttt < 2:98066/143‑1 (MQ=255) cAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTga < 2:20411/143‑1 (MQ=255) cAAACCATTCTTGATGAAGCGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTga < 1:235728/143‑1 (MQ=255) aTTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAAcc > 2:333902/1‑143 (MQ=255) ttCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCg > 2:309045/1‑143 (MQ=255) cTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCt < 2:410154/143‑1 (MQ=255) aaGTGTGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCAt < 1:198732/143‑1 (MQ=255) cgcAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACGGCGCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAAGCGCTGCCTCATCGGtt > 2:442274/1‑143 (MQ=255) cAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGCGACCCCGCTGCCTCATCTGTTTg > 2:126354/1‑143 (MQ=255) aGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGa < 1:352035/143‑1 (MQ=255) cGAAACTTGCGGGTATGTGCCCTGTGCAGACAGACCAACATGTACTGAATGTCGTGTCACACCAGCTGGATGGATCATCATCGGCGCTGATGCTTT‑GCGTCGCTGAAAGGTCTGAGCAAACGCAACCTCATCTGTTTGAagca > 2:46175/1‑143 (MQ=255) aCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCgg < 1:593325/143‑1 (MQ=255) gCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCCTTGATCAGCAGCGGCGCTAATGCATT‑GCGCCGATGAAGGGTTTGAGAAACCGCTGCCTCACCTGTTTGAAGCAGCGGtttt > 2:744510/1‑143 (MQ=255) gCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtttt < 1:651266/143‑1 (MQ=255) cGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCGTCTGTTTGAAGCAGCGGttttt > 2:616855/1‑143 (MQ=255) aTGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAt < 1:333902/143‑1 (MQ=255) aGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCtg > 1:633797/1‑143 (MQ=255) actgaccAACACGTTCTTGATGTCGTGTCACACCAGTTGCTGGGCTCATCATCGGCGCTAATGCATT‑GCTCCGATGAAGGTTTTGAGAAACCGCTGCCTCAGCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCtgtg < 2:320217/140‑1 (MQ=255) gaccAACATGTTCTGAATGGCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTgggg < 1:83649/143‑1 (MQ=255) aaCATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGAATTCCCCCTGTGGGGTaca > 1:556982/1‑141 (MQ=255) tCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGGAGCAGCGGTTTTTTTAATGGGATTCCCCCTGTGGGGTAAGCGTGAGt > 1:197561/1‑143 (MQ=255) cTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGtt < 2:238198/143‑1 (MQ=255) tGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTc < 1:233909/143‑1 (MQ=255) gAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCa < 1:445924/143‑1 (MQ=255) tCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTGTTTTGAGGGGAATCACCCAGGGGGGGGAGCTGGAGTTGaaggag > 1:256189/1‑139 (MQ=255) cGTGTCACACCAGCTGCATTGCTTATTATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAgc < 2:128221/143‑1 (MQ=255) cGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTGTTAATGGGATTCACCCTGTGGGGTAAACGCGAGGTCAATAAgc > 2:30248/1‑143 (MQ=255) cacaGCAGCTGCATTGATCATCATCGGCGCTAATGCATG‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGAt < 1:207196/143‑1 (MQ=255) cacCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGGTTCACCCTGTGGGGTCAACTTGAGTTCAATGAGCGCGATgg > 1:132628/1‑143 (MQ=255) gCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCtttt < 1:126354/143‑1 (MQ=255) gCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCtttt > 1:794487/1‑143 (MQ=255) cATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTCTATCATCGGCTTCTCCCGGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGa < 1:766439/143‑1 (MQ=255) aTTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCGGTGTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACGTGAGTTCAATAAGCGCGATGGCTTTTGGGAt > 2:643043/1‑143 (MQ=255) atcatcatcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTGGAGTTCAATAAGCGCGCGGGCTTTTTGGAtgcca > 2:84437/1‑139 (MQ=255) tcatcatcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGccc > 2:454197/1‑143 (MQ=255) cttcatcGGCGC‑AATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCg > 1:39625/3‑143 (MQ=255) atcatcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTAACTAAAGGAGCGGCTTTTTTATTGGGATTCACCCTGTGGGGCAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGc > 1:373596/1‑143 (MQ=255) catcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCAt < 1:406344/143‑1 (MQ=255) atcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGCAGCAGCGGTGTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAGGCGCGATGGCTTTTTGGCTTGCCCGCGTg > 2:552731/1‑143 (MQ=255) atcGGCGC‑AATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATg > 1:733310/1‑143 (MQ=255) cGGCGCTAATGCATT‑GCGCGGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGt < 2:384307/143‑1 (MQ=255) ggCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTg > 1:523831/1‑143 (MQ=255) ggCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTg > 1:202125/1‑143 (MQ=255) gccaaTGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTATGCTGTGATTGCCGAAGGAAAAATGAAGTTTTTGCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGAcc < 2:169777/140‑1 (MQ=255) aTGCTTT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGt < 2:792755/143‑1 (MQ=255) gt‑GCGCCGATGAAGGTGTTGGGAAGCCGCGGCCTCGTCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAAGAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgcg < 2:625442/142‑1 (MQ=255) t‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgcgg < 1:653651/143‑1 (MQ=255) | GATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGG > NC_000913/1987312‑1987592 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |