Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 481,225 T→G 7.7% intergenic (‑517/+29) tomB ← / ← acrB protein that modulates Hha toxicity/multidrug efflux pump RND permease AcrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913481,2250TG 7.7% 110.9 / 2.1 42intergenic (‑517/+29)tomB/acrBprotein that modulates Hha toxicity/multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base T (16/22);  new base G (2/2);  total (18/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

CGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACC  >  NC_000913/481086‑481359
                                                                                                                                           |                                                                                                                                      
cgcTACTTAATATAAAGTAACTAACAATCAGTAGTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAc                                                                                                                                     <  1:275323/143‑1 (MQ=255)
atcTACATAATATACATTAACAAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAc                                                                                                                                     <  1:366690/141‑1 (MQ=255)
 gcTACTTAATATAAATTAACTAACAATCAGTATTTTTTCTGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTCAGTTGGTGGTTCAATTACTCCTTAATGTGCGTAGCTTATGCATCAACAAGGCCGCTCAcg                                                                                                                                    >  2:236870/1‑143 (MQ=255)
  cttcTTAATATAAATTAACGAACAATGAGTATTTTTTATGAATTTTCTCCGTGGTTAATACTTGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAcgc                                                                                                                                   <  2:253799/140‑1 (MQ=255)
       tAATATAAATTAACTAACAATCAGTATTTTTTATGAATGGTGTCGGGGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCt                                                                                                                              >  1:554082/1‑143 (MQ=255)
         atatAAATGAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTa                                                                                                                            <  1:402967/143‑1 (MQ=255)
          tataAACTAACTAACCCTCAGTATTTTCTATGAACTTTATCCGTGGTTAATACCGGTTTTCGTCTGCGCGCCGGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCtttt                                                                                                                           <  2:437669/143‑3 (MQ=255)
           ataAATTAGCTTGCTGTCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGt                                                                                                                          <  1:213447/143‑1 (MQ=255)
           ataAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGt                                                                                                                          <  1:617355/143‑1 (MQ=255)
                              gTATTTTTTATGGATTTTTGTCGGGGTTATGACTGGTTTTCGTGTGAGATCGTTAGTTGGTGGTTCAAGTACTCCTTAATGTTCGTAGGTTATGCATAAAAGAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAat                                                                                                       <  2:735187/143‑1 (MQ=255)
                              gTATTTTTTATGAATTGTATCCGCGGTTGATACTGGTTTTCGTATGAGATCCTGAGGTGGTGGTTCAAGTACTCCTTAATGTTCGTAGCTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAat                                                                                                       <  2:359821/143‑1 (MQ=255)
                               tATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatg                                                                                                      >  1:85298/1‑143 (MQ=255)
                                       aTGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGa                                                                                              <  2:27466/143‑1 (MQ=255)
                                         gAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACa                                                                                            <  1:251450/143‑1 (MQ=255)
                                            ttttATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTAGCAAGGATGATCGACAGTa                                                                                         >  2:760406/1‑143 (MQ=255)
                                                       attAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATCACACGTTGTATCAATGATGATCGACAGTATGGCTGTGccc                                                                              >  1:294293/2‑141 (MQ=255)
                                                                  ttttCGTGTGAGATCCTGAGTTTGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCAt                                                                   <  2:62376/143‑1 (MQ=255)
                                                                  ttttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCTTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCAt                                                                   <  2:537441/143‑1 (MQ=255)
                                                                     tCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCt                                                                <  2:459894/143‑1 (MQ=255)
                                                                           gagaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGc                                                          >  2:119188/1‑143 (MQ=255)
                                                                              aTCCTGAGTTGGTGGGTCAATTGCTGCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTaa                                                       <  2:299129/143‑1 (MQ=255)
                                                                               tcttGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTaaa                                                      <  2:206609/140‑1 (MQ=255)
                                                                               tCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGATCGTAATCTTCATTCTTGCGGCTaaa                                                      >  2:522681/1‑143 (MQ=255)
                                                                                ccTGAGTTGGTGGTTCAATTGCTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAg                                                     <  2:393031/143‑1 (MQ=255)
                                                                                 cTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCGCCAAATATTATTTCTTGCGGCAAACgc                                                    >  2:195038/1‑143 (MQ=255)
                                                                                   gAGTTGGTGGGTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg                                                  <  2:746323/143‑1 (MQ=255)
                                                                                    aGTTTGTTGTTCAATTGCTCCTTGATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAAGGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc                                                 <  2:486848/143‑1 (MQ=255)
                                                                                      ttGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg                                               >  1:151424/1‑143 (MQ=255)
                                                                                         gtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACATTGTATCAATAATGATCGACAGTATGGCTGTGCTCGATATCTTAATTCTTGCGGCTAAAGCGGCGGCGa                                            >  2:628441/1‑143 (MQ=255)
                                                                                             ttCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATCTCTTCTTTCTTGCGGCGAAATCGTCGGCGAacca                                        >  1:25162/1‑143 (MQ=255)
                                                                                              tCAGTTACTCCTTAATGTGCGTAGGTTCTGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAaccac                                       <  2:90547/143‑1 (MQ=255)
                                                                                                      tCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCCCAAAGa                               >  1:268267/1‑143 (MQ=255)
                                                                                                        cTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAAt                             >  1:530470/1‑143 (MQ=255)
                                                                                                        cTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAAt                             >  1:705092/1‑143 (MQ=255)
                                                                                                               ttCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGaa                      <  1:522681/143‑1 (MQ=255)
                                                                                                                   tAGGGTATGCATAAACAGGGCCGCGTACGGGGCCTTAGTGAGTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTGGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaa                  <  2:460796/143‑1 (MQ=255)
                                                                                                                     ggTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaaga                <  2:424899/143‑1 (MQ=255)
                                                                                                                         attaagaaaaaaGGGCGTTTACGAGGTATTATGGATTACCCGTTGTATCAAGTATGTTCGATAGTATGGGTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCATCACAAAGAATACCGGAACGAAGAAGAt            <  2:720965/137‑1 (MQ=255)
                                                                                                                            cATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGc         >  1:392691/1‑143 (MQ=255)
                                                                                                                              tcactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGACCGAAGACGATTGCCa       >  1:482569/6‑143 (MQ=255)
                                                                                                                               aaaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAg      >  1:625916/1‑143 (MQ=255)
                                                                                                                                   aaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAGTGCCAGTAcc  >  1:521964/1‑143 (MQ=255)
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CGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACC  >  NC_000913/481086‑481359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑