| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 696,470 | C→T | 100% | noncoding (35/75 nt) | glnX ← | tRNA‑Gln |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 696,470 | 0 | C | T | 96.9% | 71.4 / ‑6.1 | 28 | noncoding (35/75 nt) | glnX | tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (8/18); minor base G (1/1); total (9/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG > NC_000913/696336‑696606 | aTCCGACATTGAAGGATAAGACGTGTCAACATCGCATGCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg < 1:514402/143‑1 (MQ=255) aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAATCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg < 2:569267/143‑1 (MQ=255) aGGATAAGACGTGTCAACATCGCGTTCGACATTGAATGAACGCAGCAAAGCAAAAAGCGCGCCGAAGCGAGCTTTTTTAATGGTGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGc < 2:164499/143‑1 (MQ=37) aTAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTg < 1:340996/143‑1 (MQ=255) tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg < 2:388472/143‑1 (MQ=255) aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGACCGGCGGAGGATCCGGTGCCTTACCGCTTGGCGATACCCCaa > 2:354421/1‑143 (MQ=37) cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCCACTggg > 1:218419/1‑143 (MQ=255) tCGACAGTGAATGGACGCAGAAAAGCAAAAAGCGCGCCGAAGCGCGCTTTTTTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAc < 2:187886/143‑1 (MQ=21) cgccATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTCATGTGGCTGGGGTACGAGGATTCAAACCTCGGAATGCAGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCCCt > 1:331515/4‑143 (MQ=25) cATTGAATGAACGCAGAAAAGCACAAAGCGCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCTCAACTGGGTGCACTTAc < 2:115844/143‑1 (MQ=255) ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa < 2:640798/143‑1 (MQ=255) tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg < 1:779921/143‑1 (MQ=255) tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg < 2:575903/143‑1 (MQ=255) cAATAAGCTCGCCTACGTGAGCTTTTTTAATGTGGCTGGTGTACGACGTTTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa < 2:114674/143‑1 (MQ=21) aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCTCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAGTAAAt > 1:241168/1‑143 (MQ=255) aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGTTGCACTTACAAGGTACGCGCCTTGAAtcact > 2:633568/1‑139 (MQ=21) agaaGCCCGCAGAAGCTAGCTTTTGAAATGTGGCTGGGGGACGAGGATGCGAACCTCGGGACGCCGGAATGAGACGCCGGTGCCTCACCGCTTAGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt < 2:190588/141‑1 (MQ=255) tCGCCGAGCTGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg < 2:379401/143‑1 (MQ=255) cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg < 1:397447/143‑1 (MQ=255) gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgggg < 1:58721/143‑1 (MQ=255) tttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCGAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGa < 2:397469/143‑1 (MQ=255) tttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACAAGGTAAGCGCCTTGAATAAATAGGCTGGGGTACGAGGATTCGa > 1:99315/1‑143 (MQ=255) gtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCCCTTACAATGTAATCTTCTTTAATCAAATGCACGTTTTACTATGATTCGAACCTCGGa < 1:187584/143‑1 (MQ=14) ggggTACGAGGATCCGAACGTCGGGACGCCGGAATTAGAATCCGGTGCGTTACCGCTTGGCGAGACCCCAACGGGGTGCAATTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg < 1:756963/143‑1 (MQ=38) ttgcgcggATCCGACCCTGCGACTGCCGGAACTAGAATACGGTGCCTTAGCGCTTGGCGTCACCCCAACTGGGTGCACTTTCAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGCAATGCCGGaa < 2:582278/137‑1 (MQ=25) tCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTGCATGGTCCGCGTCTTGACTAAATTGGCAGGGGTTCGAGGGTTCGACGCGCGGCGAGCCGGTATCCGCATCCg > 2:626760/1‑143 (MQ=12) gAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACACCAACTGGGTGCACTTACAAGGTCAGCGCCTTGAATAAATTGGCTGGGGTACGTGGATACGAACCTACGCCTACCGGAATCCGAATCCGGt > 2:756120/1‑143 (MQ=25) cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCAGGAATCAGAATCAGATGCCTTAACACTTgg > 1:423145/1‑143 (MQ=37) | ATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG > NC_000913/696336‑696606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |