| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,801,361 | C→A | 100% | V404F (GTC→TTC) | thrS ← | threonine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,801,361 | 0 | C | A | 100.0% | 111.5 / ‑6.7 | 37 | V404F (GTC→TTC) | thrS | threonine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (16/20); total (16/21) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGACTAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTA > NC_000913/1801228‑1801494 | ttttCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGAt < 1:6013/143‑1 (MQ=255) gcgcAACCGCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGGGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTa < 2:746640/143‑1 (MQ=255) cAACCGCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACt > 1:99133/1‑143 (MQ=255) caccAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCAt < 1:609583/141‑1 (MQ=255) gCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATc < 1:723463/143‑1 (MQ=255) gCCAGGTCCGCCTCAGCACGATCCCACATTGCGTCGCTGCCAATACGTGTTTCAGGACGAGTGGAGAGTTTGAAGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATc < 2:196662/143‑1 (MQ=255) ccGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAAtt < 1:494389/143‑1 (MQ=255) cacaTTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACagaa > 1:27010/1‑143 (MQ=255) cacaTTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACAACGATCTTCTCCAAGACAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCACGAACTTGTTCTTCAATACagat > 2:332200/1‑142 (MQ=255) tcgtcgCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATg < 1:261977/143‑1 (MQ=255) cgtcgCAGCCAACACGTCTGTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATgc < 1:177689/143‑1 (MQ=255) tcgCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATgcgc < 1:236578/143‑1 (MQ=255) tagCTGCCAATACGTTTTTCAGGACGAGAGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGGACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATgcgc > 2:695894/3‑143 (MQ=255) ccAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGAGCTTCTCGAAGCCAAAAGTGCTGTACGTATCATAGAATAAACTGATACATGCCGTCACATCATCGCGAATATGTTCTTCACTACAGAACATATGcgcgctcac > 2:662582/1‑138 (MQ=255) aaaccGTTTATCAGGACGAGTGGAGAGGTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCt < 1:261434/139‑1 (MQ=255) ctACGTTTGTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGTATGAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTg < 2:346814/142‑1 (MQ=255) gTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTa > 1:437527/1‑143 (MQ=255) ttgttCAGGACGAGTGGAGAGGTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTaa < 2:441068/140‑1 (MQ=255) tttCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATATGTTCTTGGGTTCGTAAGATATGCGCGTCATCCTGGGTAAAt < 1:342164/143‑1 (MQ=255) tttCACGCCGAGTGGAGAGTCTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCCTCATCCCGGGTAAAt < 2:397769/143‑1 (MQ=255) cAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCAGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCca > 2:93854/1‑143 (MQ=255) gAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCcacgcacg > 1:185885/1‑143 (MQ=255) gTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCAcgcg > 2:687994/1‑143 (MQ=255) agagTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATc > 1:606256/1‑143 (MQ=255) acgacgATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATg > 1:575324/1‑143 (MQ=255) acgacgATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCAGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGCGTAAATCCACGCACGCGCCTCAGGCCATg > 1:312368/1‑143 (MQ=255) cttctCGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATGCAGCGAAc < 1:548744/143‑1 (MQ=255) ctcGAAGCCAAAAGTGCTGTCCATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATGTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATGCAGCGAACCTg < 2:540711/143‑1 (MQ=255) ctcGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATGCAGCGAACCTg > 1:617126/1‑143 (MQ=255) tcGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATGCCGCGAACCTGa > 2:213998/1‑143 (MQ=255) tcGAAGCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATGCAGCGAACCTGa < 1:675467/143‑1 (MQ=255) gCCAAAAGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGAGCATCAGGCCATGCAGCGAACCTGACCGCt > 2:71751/1‑143 (MQ=255) ccAAAAGTGCTGTACATACCATAGCATAAACGGATACTTCCGTTAACTTCATCGCGCATTTCTTATTGAGTGCAGAAGATATGCGCGTCATCCTGGCTAAATCCACCCGCGCGTATCTGGCCATGCAGCGAACCTGACGGCTc < 2:437527/143‑1 (MQ=255) aaGTGCTGTACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATGCAGCGAACCTGACGGCTCGTTa < 1:141141/143‑1 (MQ=255) cTGTACAATTCATACACTACCCGGACACATCCGTGAACTTCATCGCGGATGTGTGCTTCCGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCCCGCACGCGCATCAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTg < 1:716526/143‑1 (MQ=255) tACATATCATAGAATAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCa > 1:560376/1‑143 (MQ=255) tatCAGAGAATAACCGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTa < 1:695894/143‑1 (MQ=255) | TTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGACTAAACGGATACATCCGTTAACTTCATCGCGAATTTGTTCTTCAGTACAGAAGATATGCGCGTCATCCTGGGTAAATCCACGCACGCGCATCAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTA > NC_000913/1801228‑1801494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |