| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 151,522 | G→T | 8.8% | A26A (GCC→GCA) | yadK ← | putative fimbrial protein YadK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 151,522 | 0 | G | T | 8.8% | 139.1 / 6.3 | 58 | A26A (GCC→GCA) | yadK | putative fimbrial protein YadK |
| Reads supporting (aligned to +/- strand): ref base G (28/25); new base T (2/3); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01 | |||||||||||
GTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAA > NC_000913/151380‑151664 | ttggATAACTTTCAGGAGCAACATTACCTGAAAAATACCCCCCCCCAACGAAGCCAAGATCAATATTACCCTCTTATATGATTCCTCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATg < 1:114836/140‑1 (MQ=255)gTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCCGGTGCTGTTTCTTACATTGGCAACAAGGTCACTATCCTGTCCAGCAATg > 2:278788/1‑143 (MQ=255) cTTTCAGGAGTAACATTTCCTGAAAAATATCGCACCCCAACGACGCCTAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACa < 2:652192/143‑1 (MQ=255) cTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACa < 2:258178/143‑1 (MQ=255) tttCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACGCTGGTTACTGATTCTGCTTTTGCAGGTGCTGTCTTTTACATTGGAAACACGGTCGATATCGTGTCCGGCACTGGcggtaggt > 2:550797/1‑136 (MQ=255) tttCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACAg < 1:520199/143‑1 (MQ=255) gAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATg > 1:15640/1‑143 (MQ=255) aTTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACa > 1:170235/1‑143 (MQ=255) aCCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGCCGATGCAAAACAGTa > 2:424976/1‑143 (MQ=255) aaaaaTATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTGATGac > 2:106810/1‑143 (MQ=255) aaaaaTATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGATTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGac > 2:220253/1‑143 (MQ=255) aaaaTATCCCACCCCCACGACGCCAAGAGCAATATTACCCTGGTTACTGATTCCGCTGTTGCAGGTGCTGTTTTTTACACTGGCAACAATGTCAATATCCTGTCCAGCAATGGCTGGCCAGGCGATGCAAAACAGTAATGaca < 1:259797/143‑1 (MQ=255) aaaaTATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCTAGCCATGGCTGGACAGGCGATTCAAAACAGTAATGaca < 1:374806/143‑1 (MQ=255) cccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacag < 1:369465/143‑1 (MQ=255) ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCTTTGCAACACGGTAGTGaccgaaacacc > 1:585175/1‑141 (MQ=255) aaCGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtc > 1:305927/1‑143 (MQ=255) aaCGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGAAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGAAAGGCCATGAAAAACAGTACTAACAGAAACAGAattattg > 2:583659/1‑142 (MQ=255) acgacgCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtct < 1:600493/143‑1 (MQ=255) acgCCAAGATCAATATTACCCTGGTTACGGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCtt < 2:316060/143‑1 (MQ=255) acgCCAAGATCAATATTACCATGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCtt < 2:383381/143‑1 (MQ=255) cgCCAAGATCAATATTGCCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtc < 2:803150/143‑1 (MQ=255) gCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAGTGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtca > 1:246953/1‑143 (MQ=255) cAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatc > 1:307339/1‑143 (MQ=255) cAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAAAATATTTAATTTCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACGGCATTATTCTCTtcatc > 1:114315/1‑143 (MQ=255) cAAGATCAATATTACCCTGGTGACTGAGTCCGCTTTTGCAGGTGCTGTTTTTTACAGTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatc < 1:419136/143‑1 (MQ=255) tCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCtt > 1:325897/1‑143 (MQ=255) tCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGGTATGACAGAAACAGAATTATTCTCTTCATCAGCtt > 1:760765/1‑143 (MQ=255) tatTACCCTGGTTACAGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAACCAGTAATGACAGAAACAGAATTATTCTCCTCATCAGCTTACGt > 2:677970/1‑143 (MQ=255) tACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAACCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGa < 1:153802/143‑1 (MQ=255) gcccTGATTACTGAGTCCGCTTTTGCAGGGGCTGTTTGTGACATTGGCAACAAGGGGAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAg < 1:595022/142‑1 (MQ=255) aCCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAg < 2:15640/143‑1 (MQ=255) cccTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACCGAATTATTCTCTTCTTCAGCTTACGTTGAgg > 1:179042/1‑142 (MQ=255) gTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGAt < 2:758341/143‑1 (MQ=255) tccTGATTCCGCTGTTGCAGGTGCTGGGTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGc < 2:513378/141‑1 (MQ=255) aCTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCa > 2:60662/1‑143 (MQ=255) cTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCat > 1:272507/1‑143 (MQ=255) aTTCCGCTTTTGCAGGTGGTGTTTGTTACATTGGCAACAAGGTCAATATTCTGTCCAGCAATGGCTGGAGAGGCGATGCAAAACAGTAATGACAGAAACAGAATAATTCTCTTCAGCAGCTTACGTTGAGTGGGATGCatata < 2:14558/143‑1 (MQ=255) gCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTCGAGTGGGATGCATAAATAGtt > 2:268216/1‑143 (MQ=255) cTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTc < 2:431958/143‑1 (MQ=255) ttGCAGGTGCTGTTTTGTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTg < 1:711044/143‑1 (MQ=255) cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAAt > 1:685414/1‑143 (MQ=255) ggTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCt < 2:470644/143‑1 (MQ=255) ggTGCTGTTTTGTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCt < 2:666155/143‑1 (MQ=255) gTGCTGTTTTTTACATTGGCAACAAGTTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTg > 2:705523/1‑143 (MQ=255) tGCTGTTTTGTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGt < 2:152647/143‑1 (MQ=255) cTGTTTTTTACATTCGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTCATGACAGCAACAGAACTATTCTCTTCATCCGCTTACGTCGAGTGGGACGGATATACAGTCCCTCAATCTCTat > 2:29636/1‑142 (MQ=255) cTGTTTTTTACAGTGGCAACAAGGTCAATATCCTGGCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa < 1:627487/143‑1 (MQ=255) tACATTGGCACAAGGGTGAATATCCTGCACAGCACTGGCTGTACAGGCGATGCAAAACAGTAATGAGAGACACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgtta < 2:432638/143‑1 (MQ=255) ttGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGAGATGCATATATAGTTCCTGAATCTGTAAGTCTgttagtta > 1:287098/1‑143 (MQ=255) aacaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGt > 1:39521/1‑143 (MQ=255) aGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAATATGTATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACt < 1:801772/143‑1 (MQ=255) gTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCACACCAGTACTGACAGACACAGAATTATTCTCTTCATCAGCTTCCGTCGAGTGGGATGCATATATAGTTCCTGCATCTGTAAGTCTGTGCGTTATCGGttcgtgt > 2:441072/1‑138 (MQ=255) tCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAAtgtg > 1:80099/1‑143 (MQ=255) ccTGTCCAGTAATGGCTGGTCATGCGATGCAAAAGAGTAAGGACAGAAACAGAATTATTCTCTTCATCCGCCTACATTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTgg < 2:702062/143‑1 (MQ=255) ccTGTCCAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGGATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAAtgttg > 2:205449/1‑141 (MQ=255) cTGTCCAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTAAGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTCGTTATAGGTTACTGTAAATGTGGc > 1:572617/1‑143 (MQ=255) gcAGCAATGGCTGGACAGGCGATGCAGAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGGTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGt < 2:736832/142‑1 (MQ=255) aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCCGGTGc > 1:683911/1‑143 (MQ=255) aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGATGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGGTAGTTATAGGTTACTGTAAATGTGGCTGGTGc > 1:751606/1‑143 (MQ=255) gCAATGGCTGGACCGGCGCTGCACAACAGTAATGGCAGAAACAGATTTCTTCTACTCATCAGCTTACGTTGCGTGGGATGCATATATAGTTCCTGACTCTGTAGGGCGGTCAGTGTTAGGTTACTGTAAATGTGGCTGGTGCa < 2:611629/143‑1 (MQ=255) ggCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaaa < 2:305927/143‑1 (MQ=255) | GTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAA > NC_000913/151380‑151664 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |