| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,334,704 | G→C | 10.4% | intergenic (+23/‑205) | ispB → / → sfsB | all‑trans‑octaprenyl‑diphosphate synthase/putative transcriptional regulator SfsB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,334,704 | 0 | G | C | 10.4% | 109.1 / 4.4 | 44 | intergenic (+23/‑205) | ispB/sfsB | all‑trans‑octaprenyl‑diphosphate synthase/putative transcriptional regulator SfsB |
| Reads supporting (aligned to +/- strand): ref base G (21/18); new base C (2/3); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
GCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTA > NC_000913/3334567‑3334846 | gCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgcgcgc < 2:172199/143‑1 (MQ=255) cGTGCCGGGGAAGAAGCAGACATAGCCATCGCAGCGTTACAGGTGCTCCCGGACGCCGCTTGGCGAGACGCAGTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGcgcgcg < 2:242182/143‑1 (MQ=255) atgtcGAGAAAGAAGCAGAAAAAAACATAGCAGCGTTATAGTTGTTACCGGACAACCCGTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCTGCGCgg < 1:322496/139‑1 (MQ=255) gCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCTGCGCGGGa < 1:674781/143‑1 (MQ=255) ccGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTCCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCTGCGCGGGAt < 2:452006/143‑1 (MQ=255) gaagaaGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTc < 1:571107/143‑1 (MQ=255) gaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAGTCGACACCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAg > 2:567749/1‑143 (MQ=255) aCAAAGCCATCGCAGCGTTACAGGTCCTCCCGGGCACCCCTAGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTc < 1:297902/143‑1 (MQ=255) gCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCCTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAAGCGCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTGCCATaa > 2:138947/1‑143 (MQ=255) aTCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCGTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGGTGGTTCCAGTGACTTCCATcaaaa > 1:237831/1‑143 (MQ=255) cgcgGCGTTACGGGTGCTCCCGGACCCCCCTTGGCAAGAAGCACTCATCGGCCTCGCGCACATCGCTGGTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAaca < 2:485104/143‑1 (MQ=255) cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAaca < 1:547465/143‑1 (MQ=255) cAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACCTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCCTCCCGCGCAGCGCGCGGGAGGGTTCCAGTCAGGCCCATAAAAACCCt > 2:83659/1‑143 (MQ=255) gTTCCAGGTGCGGCCTGGCCCCCCTTGGCGAGAAGCGCTCATCGGCCTCGCGCACATCGCTGTGCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAtt < 2:234120/143‑1 (MQ=255) ttACAGGTGCTCCCGGAGACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCCCACCGCGCGGGAGGGTTCCACTCAGTACCCGCAAAACACTTATTc > 2:707114/1‑143 (MQ=255) aCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAg > 1:778885/1‑143 (MQ=255) gTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCCGctct > 1:533270/1‑143 (MQ=255) gTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGctct > 1:409555/1‑143 (MQ=255) cGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGGGCCAGTAAGACCTGTTTAAACACAGATCGAGCTCGATAacac > 2:564060/1‑143 (MQ=255) ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACATGaacaa > 2:332885/1‑143 (MQ=255) cTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATCACACCTGcaaaag > 1:499613/1‑142 (MQ=255) tGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAg < 1:567749/143‑1 (MQ=255) aGCACTCAGCGGCCTCGCGCACATCGCTGGTCAACGCGATCGTTAATCCCCTCCCCTCGTCCCGCGCAGGGCGCGGGATGGTTCCAGTAAGTTCCCTAACAACCCTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtgt < 1:300374/143‑2 (MQ=255) acccctCGTCCTCGCGCCCATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGTGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACCCCTGAAAAATAGGGATGTGCATc < 2:717692/138‑1 (MQ=255) aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTta < 1:500750/143‑1 (MQ=255) tCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTc > 1:212299/1‑143 (MQ=255) tCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTACATCTTCATATATTc > 2:204807/1‑143 (MQ=255) cgcgCACCTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCt < 1:271394/143‑1 (MQ=255) gcgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATACTg > 2:544706/1‑143 (MQ=255) gCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTCCATCGTTATATATTCTGAATATTcac > 2:514020/1‑143 (MQ=255) ttCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATACAAACACTTATTCAGCTCTATAACACCTGAAAACTAGGGATGTGCATCTTTATATATTCTGAATATTCACActc > 2:766465/1‑143 (MQ=255) ttCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCACAAAAACACTTATTCAGCTGTATAACACCTGAAACATAGGGAGGTTCATCTTTATATATTCTGAATATTCACActc > 2:190793/1‑143 (MQ=255) cgccATCGTTACTCCCCTCCCCTCATCCCGCGCCGCGCGCGTGATGGTTCCATTAAGTTCCATAAAAACACTTATTCAGCTCTATACCACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCCCACTCTTTAc < 2:622627/143‑1 (MQ=255) gcgATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACa < 2:778885/143‑1 (MQ=255) gTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATCTATTCTGAATATTCACACTCTTTACAGGCACt > 1:248633/1‑143 (MQ=255) gTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATCTTCCCACTCTTTACAGGAACt > 1:248580/1‑143 (MQ=255) aaTCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtttt < 1:441168/143‑1 (MQ=255) gaaccATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACACTTTACCGCAACCTTTTCGAGCaac > 2:187998/5‑142 (MQ=255) cctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa > 1:235655/1‑143 (MQ=255) aTCCCGCGCAGCGGGCGTGATGCTTTATGCCTGCTCCACAAACACACTTATTCAGCTCTATAACACCTCAACAAGAGGGATGTGCATCCTTATATACTCTAAAGATTCACCCACTTTACAGGAACTGTTTAGAGCAAAAGGcc > 2:790457/1‑143 (MQ=255) gcgcAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATCTACTCTGAATATTCACACTCTTTACAGGAACTATTTAGAGCAATAGGCCCTCAg > 1:457525/1‑143 (MQ=255) ggaGCGCGCGGGCTGGTGCCAGTAAGTGACATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACACGTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa < 2:509224/141‑1 (MQ=255) gcAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa < 1:593819/143‑1 (MQ=255) gcAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa < 1:11013/143‑1 (MQ=255) aGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATCACACCTGAAAAATAGGGATGTGCAACTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGt < 1:99164/143‑1 (MQ=255) gcgggcggGAGCGTGCCAGGTAGTGCCAGAAGGAGACTTGTCCAGCTCTATAACACATGAAAACTAGGGATGTGCGTCTTTATATATTCTGCATATTCACACTCTGTACAGGAACTCTTTAGAGCAATAGGCCATCAGGAGta < 2:767987/143‑1 (MQ=255) | GCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTA > NC_000913/3334567‑3334846 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |