| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,196,203 | C→T | 100% | intergenic (‑104/+129) | thiC ← / ← rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,196,203 | 0 | C | T | 98.4% | 106.0 / ‑6.3 | 36 | intergenic (‑104/+129) | thiC/rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (14/21); minor base A (0/1); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAG > NC_000913/4196063‑4196339 | cgcgTTGTTCGCTGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGc < 1:394069/143‑1 (MQ=255) gTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCa < 2:622718/143‑1 (MQ=255) gttgCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCcgcg < 1:79116/143‑1 (MQ=255) gttgCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCcgcg < 1:394065/143‑1 (MQ=255) cAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCCGacc > 2:135686/1‑141 (MQ=255) gACATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATccc < 2:222937/143‑1 (MQ=255) cTCATTCCACACAGTTAAGGACGTGGCTTGTCATACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTACCTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATaaa < 1:699631/143‑1 (MQ=255) ttCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGt < 1:478571/143‑1 (MQ=255) aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGCCGATCGCCGCGGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTCTTCGCCTGATCAGGTTCCGCGGCTCGTGAATAAACGCTCGCa > 2:733705/1‑143 (MQ=255) aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACggacgca > 2:101149/1‑138 (MQ=255) aaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGcc < 2:271723/143‑1 (MQ=255) aaGGACGTGGCTTGTCCGACGACGGATGAGGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTa < 2:208067/143‑1 (MQ=255) aaGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCGGATCAGGGTCCGCGGATCCCGAACAAACGGTCccagccaggca > 2:560005/1‑140 (MQ=255) ggACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAg > 2:739880/1‑143 (MQ=255) gACGTGGCCTGCCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAgg < 1:499227/143‑1 (MQ=255) tCAGACGACGGATGCAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAACCGGTCTCAGCCAGTTAAGGCACTCCGACaa > 1:313332/1‑143 (MQ=255) aaGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGGTCAGGCACTCCGACAAGAAATTAGCCCCGc > 1:357536/1‑143 (MQ=255) aCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGc < 1:398566/143‑1 (MQ=255) aCGATAGACGCAGGCGCGATGCGATAGCAGATTCACTCTTGTTCCCTTTGCAGGGATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGc < 1:542776/143‑1 (MQ=255) accgccgcAGGGGCGATGCCATAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAACCGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCAtt < 1:462499/141‑1 (MQ=255) tgcgatAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCACATGGGGCATTGAATGTAAATTAcgcg > 2:570706/1‑143 (MQ=255) tgcgatAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTAcgca > 2:81631/1‑142 (MQ=255) gatAGCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCAGAATAAACGGTCTCAGCCCGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTGcgcgcga > 1:254448/1‑140 (MQ=255) agaGCAGATTACCTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTaa < 1:727233/141‑1 (MQ=255) aGCAGATTAACTCGTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCTGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACa < 1:64989/143‑1 (MQ=255) aGCAGATCAACTCTTGTTCCCTTTGCAGGAATTAGCTTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCGGCCAGTTAAGGAACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACa < 2:302525/143‑1 (MQ=255) gCAGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGAGTCCGCCGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAg < 2:354522/143‑1 (MQ=255) aGATTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCACAGACAATTAGGTCGCTCCGACAAGAAATAAGCCCCGCAAATGGGGCAGTGAATGTAAATTACGCGTTAACcgcg > 2:653180/1‑143 (MQ=255) aTTCACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCa < 2:806121/143‑1 (MQ=255) aTTAACTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCa < 1:513005/143‑1 (MQ=255) tAACTCTTGTTCCCGTAGCAGGTATTAGCCTGAGCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGa < 1:324776/143‑1 (MQ=255) aaCTCTTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGaa < 1:769199/143‑1 (MQ=255) tctTGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTc > 1:478683/1‑143 (MQ=255) tGTTCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAg > 2:38920/1‑143 (MQ=255) ttCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCa > 1:627970/1‑143 (MQ=255) tCCCTTTGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAg < 1:256916/143‑1 (MQ=255) | CGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAG > NC_000913/4196063‑4196339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |