Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 285,882 T→G 10.7% I163S (ATC→AGC)  yagG → putative D‑xylonate transporter YagG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913285,8820TG10.7% 104.8 / 5.3 47I163S (ATC→AGC) yagGputative D‑xylonate transporter YagG
Reads supporting (aligned to +/- strand):  ref base T (16/25);  new base G (3/3);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

TCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAG  >  NC_000913/285741‑286021
                                                                                                                                             |                                                                                                                                           
cgcttccccTCGTCTACTCCTTCCTAAACGTGCCGTACGGCGCCACGCCGGGCGTCACCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATc                                                                                                                                            <  1:177562/137‑1 (MQ=255)
   tGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATcgcg                                                                                                                                         <  1:575508/143‑1 (MQ=255)
         tGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCg                                                                                                                                   >  1:470588/1‑143 (MQ=255)
             cTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTgg                                                                                                                               <  1:463617/143‑1 (MQ=255)
             cTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGATGCCGCTga                                                                                                                               <  2:361742/143‑2 (MQ=255)
                cacCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGa                                                                                                                            <  1:649651/143‑1 (MQ=255)
                 cccTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCGCGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAg                                                                                                                           <  2:650265/142‑1 (MQ=255)
                  ccTTCGATAACGTGTCTTACTGCGCCTTGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGc                                                                                                                          <  2:191355/143‑1 (MQ=255)
                   cTTCGTCACCGTGCCGTACGGCGCCATGGCGGGCGTCATGACAGCCGACCGGAAAGAGCGTCGCGCCCGGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTAGCAGCGGCATCGCGCTGCCGCTGGTGAGca                                                                                                                         <  2:27926/143‑1 (MQ=255)
                      cGTTACCGTGCCGCACTGCGCCACGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGcatca                                                                                                                      <  1:434450/143‑1 (MQ=255)
                           aCGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGACAGAGCGCCACGCCCTTCAGCCCTCGCGCTACCGCCTTGCGGCGGCGGGCTCGCTCGCTATCAGCGGGATCGCGCGGCCGCTGGTGGGCATCATCGGc                                                                                                                 >  1:186785/1‑143 (MQ=255)
                             gTGCCGTACTGCGCCATGCCGGGCGGCATCGCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCGTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCaa                                                                                                               <  2:284364/143‑1 (MQ=255)
                              tGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCaaa                                                                                                              <  1:140867/143‑1 (MQ=255)
                              tGCCGTACTGCGCCAGGCCGGGCGTCATCACCGCCGACCCGAAAGGGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCaaa                                                                                                              <  1:132408/143‑1 (MQ=255)
                               gCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCGTGGCGCTTCTTCCTGGCGGCGGCGGACTCGCTCGCTATCAGCGGGATCGCGCTGGCGATGGTGAGCATCATCGGCacgg                                                                                                             >  1:131678/1‑140 (MQ=255)
                                   caCTGCTCCCCGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGa                                                                                                         <  2:516892/142‑1 (MQ=255)
                                      tGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGa                                                                                                      <  2:262901/143‑1 (MQ=255)
                                           cATGCCGGGCGTGATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTAGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCGGCTGGTGAGCATCATCGGCAAGGGGGACGTGCTgg                                                                                                 >  1:271196/1‑143 (MQ=255)
                                               ccGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTggg                                                                                             <  1:279531/143‑1 (MQ=255)
                                                cGGGCGTCATCCCCGCCGCCCCGAAAGAGCGGCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGc                                                                                            <  2:375201/143‑1 (MQ=255)
                                                  ggCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTa                                                                                          <  2:75279/143‑1 (MQ=255)
                                                              gccgACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCCTCGCGCTGCCGCTGGTGCGCATCATCGGCAACGGGGACGAGCAGGCGGGCTACGTCGGCGccct                                                                              >  2:668374/1‑141 (MQ=255)
                                                                           agCGTCACGCCCTGCAGTCCTGGCGCTGCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCGGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTgggg                                                                 <  2:798016/143‑1 (MQ=255)
                                                                              gTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTg                                                              <  2:183236/143‑1 (MQ=255)
                                                                                       tGCAGTCGTGGGGCTTCTGCCGGGCGGCGGCGGGCTCGCTCGCGATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGGGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgtg                                                     <  1:384754/143‑1 (MQ=255)
                                                                                                 gcgcTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTATTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGctct                                           >  1:550610/1‑143 (MQ=255)
                                                                                                     ttcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGt                                       >  1:381532/1‑143 (MQ=255)
                                                                                                     ttcttcCTGGCGGCGGCGGGCGCGCGCGCGATCAGCGGCGTCGCGCGGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGt                                       <  2:112021/143‑1 (MQ=255)
                                                                                                     gtcgtcCTGGCGGGCGCGGGCGCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAGAGGGGACGAGGGGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGt                                       <  2:321917/139‑1 (MQ=255)
                                                                                                        ttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGCTGCCGCTGGAGAGAATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTg                                    <  1:71334/143‑1 (MQ=255)
                                                                                                              gcggcggcgGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGcttctt                              <  1:417646/143‑1 (MQ=255)
                                                                                                                ggcggcgtGTGCGCTCGCTATCCGCGGCAGCGCGCTGCCGCTCGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCa                            <  1:516035/143‑1 (MQ=255)
                                                                                                                 gcggcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGATCGGCGTGGTGCTGCTCTACGTCTGCGTCTTCAc                           >  1:704951/1‑143 (MQ=255)
                                                                                                                    gcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCCGCTCGACGGCTGCtgcttccggac                        >  2:634806/1‑138 (MQ=255)
                                                                                                                      gggCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCAGCGGCCAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCACGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACCGCTGCTTCTCCACGACCa                      >  2:675105/1‑143 (MQ=255)
                                                                                                                         ctcgctcgCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCCTCGGCAAACAGGACGAGCAGGTGGGCTCCTTCGGCGCCACGTGCGTGCTGCGGCTGCGCGGCGTCGTGCTGCGCTCCGCCTGCTCCTACACGACCAAag                   >  2:83886/1‑143 (MQ=255)
                                                                                                                            gcctgcgaTCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAgc                <  2:517754/136‑1 (MQ=255)
                                                                                                                             ctcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCGGCTTCTTCACGGCCAAAGAgcg               >  1:25832/1‑143 (MQ=255)
                                                                                                                              tcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCCCGACCCACGAgccc              >  1:272491/1‑141 (MQ=255)
                                                                                                                              tcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAgcgc              >  1:273229/1‑143 (MQ=255)
                                                                                                                                 tcaccAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTac           >  2:805008/5‑143 (MQ=255)
                                                                                                                                    tCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAACGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCCAcccc        >  1:53905/1‑143 (MQ=255)
                                                                                                                                     cAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGCGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCt       >  1:46080/1‑143 (MQ=255)
                                                                                                                                      aGCGACATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGCGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCtt      >  2:416535/1‑143 (MQ=255)
                                                                                                                                       gCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGGCCCAAGAGCGCGCCACCgtt     >  2:593727/1‑143 (MQ=255)
                                                                                                                                          gCATCGCGCTGCCGCTGGTGAGGATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAg  <  2:766502/143‑1 (MQ=255)
                                                                                                                                          gCATCGCGCTGCCGCTGGTGAGCAGCCTCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGCCCAAAGAGCGCTACACCTTTGAg  <  2:470588/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
TCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAG  >  NC_000913/285741‑286021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑