| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 449,135 | C→A | 100% | V503L (GTA→TTA) | cyoB ← | cytochrome bo3 ubiquinol oxidase subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 449,135 | 0 | C | A | 100.0% | 59.3 / ‑6.7 | 23 | V503L (GTA→TTA) | cyoB | cytochrome bo3 ubiquinol oxidase subunit 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (17/5); minor base G (0/1); total (17/6) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAA > NC_000913/448996‑449273 | aaCGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGCCGCCAGTCAGGTCACGATTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGc > 1:629628/1‑143 (MQ=255) cGGGGAAGAGGTTGCACACTCCAGCGTACGGCCACCCCACGGGTCGCCAGGCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCgctgg < 2:793250/143‑1 (MQ=255) ggTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAatca > 2:60043/1‑143 (MQ=255) ttGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCCGCAatcatc > 1:806382/1‑143 (MQ=255) ggCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGc > 1:758003/1‑143 (MQ=255) ccACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCCTGGTGTGGAACTGCgg > 2:531575/1‑143 (MQ=255) aCGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACTCAGCGAAATCAGTAATGGACAACTGGCTGAAAACATCAGAATGGTGTGGAACTGCGAGTCAATc > 1:24192/1‑143 (MQ=255) gCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATATACATAACGACCCGTAGAATCCTGCGCCCAACCAGTAATGCACCGCAGGCTGCAATCATCAGCATGGTGTGGACCTGCGGCTCAATCTGCTGGc > 1:373744/1‑143 (MQ=255) cACGGTGCTCGTCGCCGTCGCGAATAGAAGCGTACATCTGAATAACGAGGCAGAGACTACCCAGCGCAGTCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGGGGAACTGCGGGTCAATCTGCTGGCTCAAACGACgg < 2:793650/143‑1 (MQ=255) tcgcgAATAGAAGCGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGcc > 1:161259/1‑143 (MQ=255) gcgAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCa < 1:691578/143‑1 (MQ=255) gcgAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCa > 1:705285/1‑143 (MQ=255) tACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGAGAAAAATTATGAATTTGTCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTgg > 1:184519/1‑143 (MQ=255) aTCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCATTCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCAt > 1:754284/1‑143 (MQ=255) cTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATaa > 1:530182/1‑143 (MQ=255) gAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGCGGAACTACGGGTCAATCTGCTGGCTCAAACGACGCGTCACGCCCATGACACCCAGCACACACAATCACAAAAAAGCAAccc > 2:671703/1‑141 (MQ=255) cAGAGAACACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACgaagaa < 1:426370/143‑1 (MQ=255) gaATACCCAGCGCAATCCGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCg < 1:60043/143‑1 (MQ=255) cccAGCGCAATCAGTAATGCACCGCTGGCTGCAATAATCAGCATGGTGGGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATGAAGGCAACGAAGAAGGCgatgat > 1:33975/1‑143 (MQ=255) aGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCAAGCGGAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCAGATGATgca > 1:113692/1‑143 (MQ=255) gcgcAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCCACGAAGCAGCAGATGATccag > 2:448258/1‑143 (MQ=255) agtCAGTAGGGCCCCGCTGGCTGCGATCATCAGCATGGTGTGGGACTGCGGGTCAATCGGCGGGCTCAAACGGCGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATccagaacc < 2:654718/141‑1 (MQ=255) aGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACCGCGGGTCAATCTGCTGGCTCAAACGACGGGGCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAGGCCGCTGATccggaacccgcc > 1:250744/1‑139 (MQ=255) | AACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAA > NC_000913/448996‑449273 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |