| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 454,891 | G→T | 100% | intergenic (+102/‑242) | bolA → / → tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 454,891 | 0 | G | T | 100.0% | 85.6 / NA | 32 | intergenic (+102/‑242) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/18); total (14/18) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAC > NC_000913/454752‑455028 | ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTccc > 2:364909/1‑143 (MQ=255) tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGACCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGAc < 1:769813/143‑1 (MQ=255) gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGAGTTTATGAAAAACGGCCTGCGGGCCGTTCTGCTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATCCCGACggcc > 1:122110/1‑140 (MQ=255) gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTGTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAACTTGc < 1:681715/143‑1 (MQ=255) aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCggg < 1:251816/143‑1 (MQ=255) cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt < 1:364909/143‑1 (MQ=255) tAAGAAGGCATTTGCAACTGTCGGCGCTTTTCCGGTTTGTTGCTAAAGATTTTATGAGAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATGTTGCGCTTTTCTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa < 2:23537/143‑1 (MQ=255) tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAGACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa < 2:209043/143‑1 (MQ=255) cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgc < 1:554896/143‑1 (MQ=255) tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGa > 1:140034/1‑143 (MQ=255) tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTCTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAGTTGCCCGGGAATTGTCAAAAAATACGCGa > 2:264232/1‑143 (MQ=255) aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcg > 2:355552/1‑143 (MQ=255) gcgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCACAAAATAATGTTGAAGAGTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa > 1:182847/1‑143 (MQ=255) gcTTTTCCGTTATGTTGCTAAAGCTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTGTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAcc < 2:626703/143‑1 (MQ=255) tttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt < 1:686999/143‑1 (MQ=255) ttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCACTAACCGTtc > 2:143990/1‑143 (MQ=255) cttATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa < 1:785882/141‑1 (MQ=255) tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCCTAAAAg > 1:567467/1‑143 (MQ=255) aaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTATCGACTCATAAAAGTg > 2:329884/1‑143 (MQ=255) aaaCGGCGGGCGGGCCGTTTTGTTGTGTCGGGATTTTGCGCTTTTTTCCCAGCATGCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt < 1:277243/143‑1 (MQ=255) cGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc < 1:527910/143‑1 (MQ=255) cGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc < 1:715942/143‑1 (MQ=255) cGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGGAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATCAAAGTGATGCCGCCATAATGCCGCGTCCt > 2:756512/1‑143 (MQ=255) tttgtttggtCTGGTTTTTGCGCTTTTTTTCGAGCATCCCGACCAAAATTGGCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGATTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAAt < 2:318227/143‑1 (MQ=255) gttttgtCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCt > 1:787003/1‑143 (MQ=255) gttttgtCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCt > 2:460577/1‑143 (MQ=255) tgtCTGGATTTTGCGCTTCTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCg < 1:78270/143‑1 (MQ=255) tgtCTGGATGTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAACGCGCAATACCCGTTCTCTTCTCTTATAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCg < 1:462517/143‑1 (MQ=255) tGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCCCTATAATGCTGCGTCCTATTTGAATGCTTTCGGgat > 1:546583/1‑143 (MQ=255) ggATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTTGGgttg > 1:755509/1‑143 (MQ=255) ttGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt < 1:165606/143‑1 (MQ=255) gcgcTTTTTTCCCAGCATTCAGACGAAACTTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTgg < 2:47873/143‑1 (MQ=255) ttttttCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAc < 1:62703/143‑1 (MQ=255) | CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAAC > NC_000913/454752‑455028 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |