Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	674,768	G→T	100%	R6S (CGC→AGC)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,768	0	G	T	100.0%	101.3 / NA	32	R6S (CGC→AGC)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/18);  total (14/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAAT  >  NC_000913/674629‑674906                                                                                                                                            |                                                                                                                                           tGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTa                                                                                                                                         >  2:157944/1‑143 (MQ=255)    cccATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTg                                                                                                                                      <  1:481701/143‑1 (MQ=255)    ccaaTGTGTAGTCGACTAGACTGATAGGGAAGCATAGACAGGGAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATAAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTg                                                                                                                                      <  2:328760/140‑1 (MQ=255)     ccATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGc                                                                                                                                     >  1:123161/1‑143 (MQ=255)         gtgtAGTCGAGCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCtt                                                                                                                                 <  2:50041/143‑1 (MQ=255)         gtgtAGTCCACCAGAAGGATAGGGAAGCATATACAGGCAGTAATACTTCCCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCtt                                                                                                                                 <  2:116106/143‑1 (MQ=255)               tCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGTCTGTATTGCTCTTGCATgg                                                                                                                           >  1:404903/1‑143 (MQ=255)                   caaggaGGAAAGGGGAGCATGGACAGGCAGTAATGCGTCTCTGTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGc                                                                                                                       <  1:40812/138‑1 (MQ=255)                    cAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGcc                                                                                                                      >  1:543631/1‑143 (MQ=255)                     aGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCa                                                                                                                     >  1:456224/1‑143 (MQ=255)                           acaGGTAAGCACAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTc                                                                                                               <  1:207880/141‑1 (MQ=255)                            tAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTcc                                                                                                              >  1:374232/1‑143 (MQ=255)                                          cAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACgg                                                                                                <  1:317067/143‑1 (MQ=255)                                                   aTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGTGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACAGCTACACATg                                                                                       >  1:384324/1‑143 (MQ=255)                                                                      tCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGttttt                                                                    >  2:204022/1‑143 (MQ=255)                                                                       cTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGATGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTc                                                                   <  2:356714/143‑1 (MQ=255)                                                                       cTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTc                                                                   <  2:563972/143‑1 (MQ=255)                                                                        tttgggtACTTCAAGTGGGCGCTTCTCATCCCACTGAAGCTGTACTTTGGATTCTCTCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCa                                                                  <  2:219812/137‑1 (MQ=255)                                                                           ggTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTTTAAATGTTGCGTTGTGGTGGTTTTTcggtt                                                               >  1:193310/1‑139 (MQ=255)                                                                                   cAACTGTGCGCTTCCCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCgcatag                                                       <  1:341878/143‑1 (MQ=255)                                                                                             cTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCaa                                             >  2:222047/1‑143 (MQ=255)                                                                                              tgttcttCCCAATGAAGCCGCGCTTCGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCaaa                                            <  2:31541/137‑1 (MQ=255)                                                                                                 tcATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGt                                         <  1:157944/143‑1 (MQ=255)                                                                                                      ccAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGATGAGGTGGTTTTTCAGATCCGCAAAGCCAAGCCCAAACGGCcgcg                                    >  2:529028/1‑143 (MQ=255)                                                                                                             aGCTGTACTTTGGTTTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATACCCCAAACGTCCGCGTCAAAAc                             <  2:447671/143‑1 (MQ=255)                                                                                                               cTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGGAGCGTTGAGGTGGGTTTTCAGATCCGCATAGCATAGCCCAACCGTCCGCGTCAAAACAg                           >  2:281743/1‑143 (MQ=255)                                                                                                                tGTACTTTGTATCCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGc                          <  1:453625/143‑1 (MQ=255)                                                                                                                tGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGc                          <  1:455172/143‑1 (MQ=255)                                                                                                                 gTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGcc                         >  1:408861/1‑143 (MQ=255)                                                                                                                        gggttCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTcgcg                  <  2:77962/140‑1 (MQ=255)                                                                                                                               aTCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGCTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCGTTCGCGCACTCGa           >  2:326209/1‑143 (MQ=255)                                                                                                                                        gggCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAat  <  1:300355/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                           TGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAAT  >  NC_000913/674629‑674906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑