Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,151,736	T→C	81.7%	V41A (GTT→GCT)	acpP →	acyl carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,151,736	0	T	C	81.7%	35.1 / 2.2	21	V41A (GTT→GCT)	acpP	acyl carrier protein
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (6/11);  total (8/13)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGA  >  NC_000913/1151612‑1151877                                                                                                                             |                                                                                                                                              agTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTTAGCTGGCAATGGcgct                                                                                                                             >  2:443731/1‑143 (MQ=255)   aagaggCAGTAGCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTgg                                                                                                                           <  2:486849/138‑1 (MQ=255)             aTCGAATAGCGCGTTAAGAAAATTATCGGCGAACAGCTTGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGtt                                                                                                                 <  2:411830/143‑1 (MQ=255)                     aCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTg                                                                                                         <  1:336942/143‑1 (MQ=255)                                aaaTTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGAc                                                                                              <  1:497752/143‑1 (MQ=255)                                                         tAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCg                                                                     <  2:412359/143‑1 (MQ=255)                                                                gaagaaGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGc                                                              >  1:341516/1‑143 (MQ=255)                                                                  agaagTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTg                                                            >  1:334654/1‑143 (MQ=255)                                                                    aagTGACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCAGGCAGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGcc                                                          <  1:569978/143‑1 (MQ=255)                                                                     agTGACCAACAAGGCTTCGTTCGGTGAGGGCCTGGGCGCGGATGCTCTTGACACCGCTGAGCTGGTAATGGCGCTGGAAGAAGAGTTTGAGACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCa                                                         <  2:175471/143‑1 (MQ=255)                                                                      gTTACCAACAGTGCTTCTTTCGTTGAATTCCTGGGCGCGGATTCTCTTGACACCGCTGAGCGGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCAt                                                        <  2:457087/143‑1 (MQ=255)                                                                            aacaaTGCTTCTTGCGTTGAAGACCTGGGCGCGCATGCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTa                                                  <  1:535375/143‑1 (MQ=255)                                                                                    ttcttTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACg                                          <  1:168850/143‑1 (MQ=255)                                                                                       tttCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGCGTACATCAACGGcc                                       >  2:552296/1‑143 (MQ=255)                                                                                         tCGTTGTAGTCTTGGTCGCGTTTTCTCTTGATACAGCTGAGATGGTAATGGCTCGGGAAGAAGAGGTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGccac                                     <  1:337871/143‑1 (MQ=255)                                                                                              gAAGACCTGGGCGTGGATTCTCTTGACGCCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAGTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGc                                <  2:563165/143‑1 (MQ=255)                                                                                                  aCCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCAGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTaa                            >  2:229227/1‑143 (MQ=255)                                                                                                             gATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACAtctc                 >  2:139424/1‑143 (MQ=255)                                                                                                                    ttGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGACCATCTCCAGGCgg          >  1:345541/1‑143 (MQ=255)                                                                                                                    ttGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCgg          >  2:471479/1‑143 (MQ=255)                                                                                                                            gTTGAGCTGGTGATGGGTCTGTAAGAGGGTTTTGATACTGTGCTGCCGGACGAAGAAGCTGAGCAAATTACCACCGTTCAGGCTGCCATTGATTACATCTACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGa  <  2:453047/143‑1 (MQ=255)                                                                                                                             |                                                                                                                                              AGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGA  >  NC_000913/1151612‑1151877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑