| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,196,803 | A→C | 13.1% | intergenic (+430/+64) | icd → / ← ymfD | isocitrate dehydrogenase/e14 prophage; putative SAM‑dependent methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,196,803 | 0 | A | C | 13.1% | 50.3 / 2.1 | 26 | intergenic (+430/+64) | icd/ymfD | isocitrate dehydrogenase/e14 prophage; putative SAM‑dependent methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (9/13); new base C (2/2); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
CTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCAT > NC_000913/1196666‑1196943 | cTTACACCCGCTCATAGATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGcgcg < 2:373843/143‑1 (MQ=255) acCCGCTCAAATACTCTTGAATGGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGACAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGcccc < 2:330152/143‑1 (MQ=255) cagCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGcctttt < 1:294333/141‑5 (MQ=255) cGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACCACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCTTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGccttttt < 2:74458/143‑6 (MQ=255) cGCTCAAAGATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGCAGCGTCGAGCTGCATGGGGTGAAATCACCTTCGCCGTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTc < 1:566803/143‑1 (MQ=255) aaaTATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAGTGTACAAACGGCCGCGCGCGCGGCCCCTTCacata > 2:4831/1‑143 (MQ=255) aaTATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTGAAAAAGGCCGCGAGCGCGGCCCCTTCacatac < 1:398948/143‑1 (MQ=255) tGTTAAATTGCCGGTTTTGTATCAACTACGCACCCCAGTATGACCATCGAACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCtt < 1:561855/143‑1 (MQ=255) aaTTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCCCCCTAGCGTTCAGGTACATGCGGAATGTAAAAAACGCCCCGAGCGCGGCCCCTTCACATCCCTCTttatta > 2:201252/1‑143 (MQ=255) tCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAAGCATTGGGCGCAATCCCCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAAcc > 2:354876/1‑143 (MQ=255) tactCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGTAATCACCTTAGCGTTCAGGTACATGCGGAATGTGAAAAAGGCCGCGAGCGCGGCCCCTTGACATACCTCTTTAGTACTGAGACTGTTTAACATAggg > 2:441960/1‑143 (MQ=255) ccAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACa < 1:383973/143‑1 (MQ=255) ccAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGGACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAGTTAGTATCGTATTATGTTGCATTACa > 1:139730/1‑143 (MQ=255) gCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCa < 2:304090/143‑1 (MQ=255) gTGCCATTACCTTTGCGCTCAGGTACATGCGGAATGTAAAAATGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGa < 2:334763/143‑1 (MQ=255) tcgCGTTCAGGTACATGCGGAAGGCAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTc < 1:109120/141‑1 (MQ=255) tCAGGTACCTGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCtt < 1:293155/143‑1 (MQ=255) acctGCCGAATGTAAAAGACGCCGCGAGGGCGGCCCCTTCACATACTTCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACACCTCCCAACTGTGACATATGAACTTCCTGCTTTACTcc < 2:453994/140‑1 (MQ=255) cGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTACCAACAGTTACATATGAACTTCCTCCTTTACTCaacaac > 2:210846/1‑143 (MQ=255) aaTGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACttt < 1:89860/143‑1 (MQ=255) tGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTaa > 2:181331/1‑143 (MQ=255) tAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTGTATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAata > 1:287331/1‑143 (MQ=255) aaaaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAACTATTAGCGTATCATATTGCCTTACAACTCCCAACAGCGACATATGAACTTCCTGCTTTACTCCACGACCTTAATCttt > 2:206730/1‑143 (MQ=255) gggCCGCGCTCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGGATTATATTGCATTACAACTCCCAACAGTGGCATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCaa > 2:513237/2‑143 (MQ=255) gcgAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCa < 2:539925/143‑1 (MQ=255) cgAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCAt > 1:217972/1‑143 (MQ=255) | CTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCAT > NC_000913/1196666‑1196943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |