| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | 100% | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 97.6% | 71.0 / ‑6.5 | 29 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (2/0); major base T (11/14); minor base G (1/1); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCT > NC_000913/124101‑124378 | gtggtgGGCACGGTTCGTGGATAATCGAGGCGGCATGCGAGACAGGGGAGGAAGGCAGAGGCAAAGCGACAGTAATCCTGGCGCATACCATGAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGAACTAg < 2:10462/143‑1 (MQ=255) gtggtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGtt > 2:229067/1‑143 (MQ=255) cGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaaa < 1:29148/143‑1 (MQ=255) tCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGa > 1:108058/1‑143 (MQ=255) aaaTCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGAc < 1:113196/143‑1 (MQ=255) cTGCAGTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTACCATCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGt < 1:241463/143‑1 (MQ=255) aaGAAAGCGCAGTAAACCACAATCCAAGCGACAGTAATCCTTGCTCAGACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcg < 2:111891/143‑1 (MQ=255) aGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgc < 1:508114/143‑1 (MQ=255) aGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTc < 2:108058/143‑1 (MQ=255) ggCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCAGTTGTTAAAAACATCGCGCAGTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGGTTCAATGTGCCGgt > 1:291480/1‑143 (MQ=255) gaagacGACAGTAATCCTTGCTCAGACCATTAACGCTATTGATAGGGGTGATGCGGCTGAAGGTAAAAACATCGCGCACTAGGTGAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGgtgt < 2:251271/138‑1 (MQ=255) gACAGTAATCCTTCCCCATACCATTAAAGGTTACGGCATGAGCGACGCGTCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATg < 2:306982/143‑1 (MQ=255) gTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTCGGTTAAGAAAATGAACATGGATGGTGTGAGCCACAGCCGCGACCGTTCCAACGTGCCGGTGTCTGATGCAgc > 1:412489/1‑142 (MQ=255) tCATACCATTAAAAGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTCAGAAACTGAACATGGACGGTGGGCGTCATCTCCGCGAACGCGTCAATGTGCCGGTGTCTTATTCCGATCTCGAAAAAc > 2:534746/1‑143 (MQ=255) aTACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGGCTGATGCAGATATCGAAAAACTg > 1:522189/1‑143 (MQ=255) aCCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt < 2:438977/74‑1 (MQ=255) aCCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt > 1:438977/1‑74 (MQ=255) aCCATTAAAGGTTACGGCATGGGCGACGCGGCGGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGcc < 1:135754/143‑1 (MQ=255) aTTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACGTGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGGTGCAGATGTCGAAAGACTGCCGTa > 2:534142/1‑143 (MQ=255) aaaGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTCCac > 2:150406/1‑142 (MQ=255) cGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACGAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCTGCGACCGTTTCAATGTACCGGTGGCTGATGCAGATGTCGAAAAACTGGCCTACAGCagattca > 2:238927/1‑137 (MQ=255) taCGCGGCTGGCGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCAGATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTc < 1:150635/142‑1 (MQ=255) accgGCTGAAGGTATAACAAGCCCGAGCTAGGTTACGAAACTGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTg < 2:131638/141‑1 (MQ=255) cgGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATAGAAAAACTGCCAGTTTTCTCCTTCCCGGAAGGTTCTGaa > 1:440640/1‑143 (MQ=255) gggtGAAGGTAAAAACATCGCGCACGAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAag < 1:467420/140‑1 (MQ=255) tGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTCATGCAGATATCGAAACACTGCCGTACATCACCTTCCCGGCAGGTTCTGAAGAGc > 1:263701/1‑143 (MQ=255) gTACAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGCGTCTGATGCAGAGATCGAAAAACAGCCGTACAACACCTGCCTGGTAGGTCCTGAAGCGCATAcc > 2:408341/1‑143 (MQ=255) aCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTg < 1:465885/143‑1 (MQ=255) cgcACTGGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGCGCATACCTTTCTGCACGCt > 1:223003/1‑143 (MQ=255) | GTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCT > NC_000913/124101‑124378 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |