| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,821 | C→G | 100% | C104S (TGC→TCC) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,821 | 0 | C | G | 100.0% | 107.0 / NA | 34 | C104S (TGC→TCC) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (21/13); total (21/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCC > NC_000913/2661690‑2661948 | gcgcgcCGTGAGTATGCTGACGACCAGACACATCCAGCACTCCCTGGTTAATAAACAGTTCGCCTAAAGAACTGTTGTTGAGAGAACCGGAGAGACGGTCGCCCAACACACGCCACAGCGCGTGGCGCCGGGCTATATcgccg > 2:108650/1‑143 (MQ=255) tGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgccgcc > 1:544019/1‑143 (MQ=255) gacgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGAGTGGGTAAGGGATTTATCGCCGCCCTGGCAGCCGCATTTa > 2:152180/1‑143 (MQ=255) cgacCATACACATCAAGCACTCCCGGGTTATTAACCAGTTCGCCTAAAGTCATGTTGTTGAGCAAGCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAcc < 1:285367/143‑1 (MQ=255) acCAGATACATCCTTGACTTCATGGTTATTAACCAGTTCGCCTAAATTAATGTTGTTGACAAAACCGGTGGGACGGTCGCTCAAATCACGCCACCGCGCGTGGCTCAGGGATTTATCGCCGCCCTGCCCGCCGCCTTTACCCt < 2:21630/143‑1 (MQ=255) ccAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg > 1:519335/1‑143 (MQ=255) ttCCTGGTTATTAACCAGTTCGCCTCAAGTAATGTTGTTGTGAAGACCGGTGAGACGGTCGCTCAAATCCCGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCATGGCCGACGGCTTTACCCAGACAAGGGGTGGCGTcga > 1:265797/1‑141 (MQ=255) ttattaACCAGTTCGCCTAAAGTAGTGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTc < 1:428341/143‑1 (MQ=255) tattaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCg > 1:332028/1‑143 (MQ=255) tattaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCg > 1:332728/1‑143 (MQ=255) ccTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAAt > 1:480413/1‑143 (MQ=255) ccTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCATGCCCCTGACGACGGGTGGCATCTACAGATTCGCCAACGGCGCTAAt > 2:119158/1‑143 (MQ=255) ctcaaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAAtt < 2:348828/140‑1 (MQ=255) cTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCGAAtt > 1:419825/1‑143 (MQ=255) gttgttGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTGAGTACTTCGCCaa > 1:113027/1‑143 (MQ=255) ttgttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGACAGGGATTTATCGCCGCCCTGGCGGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCacc > 1:14948/1‑141 (MQ=255) gttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGGCAACGGCGCTAATGACTTCGCCAACgg > 1:265584/1‑143 (MQ=255) gggaAAACCGGTGAGACGGTCGCGCCAATCAAGCATACGTGCTTGCGTCTGTGATTTATCGCCGCCCTGTCAGCCGCCGTTCCCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGa < 2:251126/141‑1 (MQ=255) agaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAt < 1:4019/143‑1 (MQ=255) agaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCGACAGCTTCGTCAACGGCGCTAATTACTTCGCCAACcgcgct > 2:301646/1‑141 (MQ=255) aaaaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtgc > 1:477765/1‑143 (MQ=255) cGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGctgctg < 1:521903/143‑1 (MQ=255) tGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTGATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCa < 1:92576/143‑1 (MQ=255) gaCGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCGGGCAGCCGCCATTACCCTGACAACGGGTAGCATCTACAGATTCCTCAACGGCGCCAATTACTCCGCGAACCGCGCTGGCGCTGGCATCt > 2:125514/1‑143 (MQ=255) aCGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCAGCtt > 1:100795/1‑143 (MQ=255) ggcGCTCAACTCCCCCCGCGGCGCGTCGGTCAGGGATTGATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 2:474374/141‑1 (MQ=255) gccGCTCAAATCACGCCACAGCCCGTGGGTCAGGGATTTACCGCCGCCCATACACCGTCCGTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATGACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 1:414013/141‑1 (MQ=255) gTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCGACGGCGATGCTGCTGGCATCATTGc > 1:14023/1‑143 (MQ=255) gTCGCCCAAGTCACGCCACCGCGCGTTGGCCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 2:163572/143‑1 (MQ=255) cAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGAAGCTACAGATTGGTTGACCACACCAAGTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACa > 2:403574/1‑143 (MQ=255) cACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATaa < 1:549656/143‑1 (MQ=255) aCGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGATGCTGGCATCTTTGCCTAACAGCTAac > 1:175961/1‑143 (MQ=255) gCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGGCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATGTTTGCATCACAGATAacca > 1:317592/1‑143 (MQ=255) aGCGCGTGGGTCAGGGATTGATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGcc < 1:101086/143‑1 (MQ=255) | GCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCC > NC_000913/2661690‑2661948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |