| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 151,522 | G→T | 12.7% | A26A (GCC→GCA) | yadK ← | putative fimbrial protein YadK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 151,522 | 0 | G | T | 12.7% | 61.1 / 3.4 | 32 | A26A (GCC→GCA) | yadK | putative fimbrial protein YadK |
| Reads supporting (aligned to +/- strand): ref base G (13/15); new base T (2/2); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01 | |||||||||||
GATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAG‑TTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGA > NC_000913/151383‑151661 | caTAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCt < 1:31465/142‑1 (MQ=255) tAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTgg > 1:44244/1‑143 (MQ=255) aCTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGAc < 2:77937/143‑1 (MQ=255) cTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACa < 1:225053/143‑1 (MQ=255) cTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACa > 1:397984/1‑143 (MQ=255) tCAGGAGTACACTTACCGGAGTAATATCCCACCGCAACGACGCCAAGATCAATATTACCCTGGTTACTGCTTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGc < 2:368201/143‑1 (MQ=255) agtgccTGAAAAATATCCCTCCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACa < 2:436650/139‑1 (MQ=255) tGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGCACCGGCGATGCACAACCGTACTg > 2:135897/1‑143 (MQ=255) tCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGCCTTCACAGGCGATGCAAAACAGTAATGacagaaaca < 1:569526/143‑1 (MQ=255) cACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGACGCAAACCAGTAATGacagaaacagca > 2:544899/1‑141 (MQ=255) gCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtca > 1:7095/1‑143 (MQ=255) ccAGGATCAATATTTCCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcat < 2:519391/143‑1 (MQ=255) aaGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGCTGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatca > 2:171931/1‑143 (MQ=255) cgATCAATATTACCGTGGTTACTGATTCCGCTGTTGCAGGTGCTGTTTTGTACATTGGCAACAAGTTCAATATCCTGTCCAGCAATGGCTAGACAGTCTATATAATACAGTAATGACAGAAACAGAATTATTCTCTTCATCAg < 2:464814/142‑1 (MQ=255) aGATCAATAGTACCCTGATTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCCGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAg < 2:285434/143‑1 (MQ=255) aTCAATATTACCCTGGTTCCTGATTCCGCTTTTGCAGGTGCTGCTTTTTACATTCGCAACAAGGTCAATATCCTGTCCAGCAATGGATGCACAGGCGATGCAAAACAGTAATGACAGACACAGAATTACTACCTtcacgcact > 2:539006/1‑137 (MQ=255) cAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATGCTCTTCATCCGCTTa > 1:331272/1‑143 (MQ=255) ggTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa < 1:251061/143‑1 (MQ=255) gATTTCGCGTTTGCAGGTGCTGTTTTTTACATTGGCAACGAGGTCAATAACCGTTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACTGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCatat < 2:372344/143‑1 (MQ=255) aTTCCGCTTTTGCAGGTGCTGTTTTTTACATCGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCatata < 1:153622/143‑1 (MQ=255) gcgtttGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAG‑tt < 1:255504/140‑1 (MQ=255) cTGTTTTTTACATTGGCAACAAGGTCAATGTCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAG‑TTCCTGAATCTGTaa > 1:278558/1‑143 (MQ=255) tGTTTTTTCCATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAG‑TTCCTGAATCTGTAAg > 1:26378/1‑143 (MQ=255) tGGCAACAAGGTCAATATCCTGTCGAGCAATGGCTGGACAGGCGATGAAAAACAGTAATGACAGAAACAAAATTATTGAATATAGCGACTCACTTTGAGTGGGATGCATATATAG‑TTACTGAATCTGTAAGTCTGTTAGTtat < 2:404124/143‑1 (MQ=255) caaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATCGATGACACATTCT‑TAAGTCTGATAGTTATAGGGTa > 2:5443/1‑143 (MQ=255) ccTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAG‑TTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTgg > 1:201617/1‑143 (MQ=255) tGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAG‑TTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCt > 1:55397/1‑143 (MQ=255) gTCCAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTGGCGTGGGATGCAGATATAG‑TTCCTGAATCTGTAAGTATGTTAGGCAGAGGTTACTTGAGATGTGGCTg > 2:369306/1‑143 (MQ=255) cAGCAATGGCTGGACAGGCGCTGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGCGTGGGATGCATATATAG‑TCCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTg < 1:422026/143‑1 (MQ=255) cAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACACAATTATTCTCTTCATCAGCTTACGTTCAGTGGGATGCATATATAG‑TTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTg < 2:554487/143‑1 (MQ=255) gCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAG‑TTCCTGAATCAGAAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGATGCa > 1:239133/1‑143 (MQ=255) aaTGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAG‑TTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGa < 1:490413/143‑1 (MQ=255) | GATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAG‑TTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGA > NC_000913/151383‑151661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |