| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,596,017 | G→A | 10.0% | R129R (CGC→CGT) | livH ← | branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,596,017 | 0 | G | A | 10.0% | 94.3 / 2.2 | 40 | R129R (CGC→CGT) | livH | branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH |
| Reads supporting (aligned to +/- strand): ref base G (16/20); new base A (2/2); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
AGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTAGGCGCTGGC > NC_000913/3595875‑3596154 | aGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTcg > 1:216815/1‑143 (MQ=255) cGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTcgcg > 1:265621/1‑143 (MQ=255) tGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGaa < 2:202804/143‑1 (MQ=255) aaaaTCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGCTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCCCGGCCGCGCCACGCcgcgcgcccc > 2:503886/1‑139 (MQ=255) tCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCg < 1:336194/143‑1 (MQ=255) tCAGCGCCAGCATGGCGAGGAAGGTAACAATCAAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAGCCTTCGGTc > 2:297325/1‑143 (MQ=255) gcCGGCATGGCGAGGAAGGTAATAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCCCGTCGCGCGAACCTTCGGTCAGGCt < 1:495954/143‑1 (MQ=255) aTGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTa < 1:303694/143‑1 (MQ=255) gCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTGTGCACCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGtt > 2:484635/1‑143 (MQ=255) gCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGtt > 1:280814/1‑143 (MQ=255) ggggAAGGCAGCAACGCAGACCACCGCCTGCAGCTTGGGAAGCGAGGCCGAGAAGTGTTCCCTATGCCGCACCACCCACTGCCCGTTAAACTGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGtttt < 2:113581/141‑1 (MQ=255) aGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTg < 1:483618/143‑1 (MQ=255) ggAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCCCCCACTGACCGTTAAACAGTCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGc < 1:174200/143‑1 (MQ=255) aaTCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGCACCTTCGGCCGGGCTCACGTAGTTTGGCAGGAAGATgg > 2:271240/1‑143 (MQ=255) aaTCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCACGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATgg > 1:510526/1‑143 (MQ=255) aaTCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCACGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATgg > 1:362367/1‑143 (MQ=255) aGATCACCGCCTGCATGGTGGTAATAGAGGCACACACGTTGTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGCAGATGGACATa > 1:541110/1‑143 (MQ=255) ccGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGAtt > 1:496629/1‑143 (MQ=255) cTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGAACGTTAAACAGAATCGGCAGCGCCACGTCGCGCGAACCTTCGGTCATGCTGACGTAGGTTTGCAGGAAGATGGGCATGCCGAGTGCac > 1:106048/1‑142 (MQ=255) tGCCTGGTGGTAACAGAGGCCGACACTTTTTCGCTATGCCCTACCTCCCACTGACCGTTAAACTGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGGAGTTTGGCAGGAAGATGGACATACCGATTGCaga < 1:191161/143‑1 (MQ=255) ggtggtAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGa < 1:502098/143‑1 (MQ=255) ggtggtAACAAAGAAAGAACAATTTTGGCGATGCCCCACCACCCCCTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTTCAGAGATGa < 1:533222/143‑1 (MQ=255) ggtAAGAGAGGCAGAGAAGTGTTCGCGGTGCCCCACCACGCGCTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTg < 2:160691/143‑1 (MQ=255) agagAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGCACCTTCGGTCAGGCTGACGTAGATTTGCAGGACGATGGACATACCGATTGCAGAGGTGAGTGCAATCACgcgcc > 2:64913/1‑142 (MQ=255) cgaACTTTTCGCTATGCCCCCCCACCCACTGACCGTTTAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTa < 1:118525/142‑1 (MQ=255) agaAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTa < 1:31175/143‑1 (MQ=255) ttttCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGtt > 1:218099/1‑143 (MQ=255) tttcCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGtt < 1:63338/143‑1 (MQ=255) gCTATGCCCCACCACCCAATGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACAGACCGATTGCAGAGATGAGCGCAATCAGGCGCGCAGAGATAGGCa > 2:347199/1‑143 (MQ=255) gcaccaccaCCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGACCGTTCGGTCAGGCCGACGGAGATTTGCCGGAAGGTGGGCATGCCGATTGCAGAGATGAGTGGAATCAGGCGAGCAGGGGTCCGCACCggg > 2:542865/4‑143 (MQ=255) accaccCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTCGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTa > 1:197515/1‑143 (MQ=255) ctccccgcTGACCGTTATCCTGGCTCGGCAGCGCCACGTCGCGCGAGCCTTCGGTAAGTCGTATGGAGTGTTGCAGGAAGATGGACATAGCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTaa < 2:199409/140‑1 (MQ=255) ccacccacTGACCGTTAAACAGGCTCGGCAGCGCCACGGCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTaa < 1:401232/143‑1 (MQ=255) cacTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCAc < 1:101189/143‑1 (MQ=255) aaCAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACt > 1:82096/1‑143 (MQ=255) aaCAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACt > 1:193247/1‑143 (MQ=255) ggtgggCAGCGGTGCGTCGGGCGAGCCTGGGGTCAGGGTGGCGGAGTTTTGCAGGAGGATGGACATGCCGAGTGGAGCGAGGAGTGCAATCGGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGc < 2:234070/139‑1 (MQ=255) ggCAGCGCCGCGTGACGCCAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTa < 2:362367/143‑1 (MQ=255) ggCAGCGCCACGTCACGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTa < 2:510526/143‑1 (MQ=255) ccACGTGGCGCGAAGCTGCGGGCAGGCTGACGTGGTTGTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGGTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTAGGCGCTg < 2:265621/143‑1 (MQ=255) ccACGTCGCGCGAACCTTCGGTCAGGGTGACGTAGTTGTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTAGGCGCTg < 2:216815/143‑1 (MQ=255) cACGTCGCGCGAACCTTCGGTCAGGATGACGTAGTTTTGCAAGCAGCTGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTAGGCGCTgg > 1:82692/1‑143 (MQ=255) aCGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACCCCGGCCGCAGGCGCTGGc > 1:176670/1‑143 (MQ=255) | AGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTTCGGTCAGGCTGACGTAGTTTTGCAGGAAGATGGACATACCGATTGCAGAGATGAGTGCAATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTAGGCGCTGGC > NC_000913/3595875‑3596154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |