| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,033,610 | G→A | 62.5% | G156S (GGT→AGT) | trkH → | K(+) transporter TrkH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,033,610 | 0 | G | A | 62.5% | 9.5 / 11.3 | 22 | G156S (GGT→AGT) | trkH | K(+) transporter TrkH |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (11/2); minor base T (3/5); total (15/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGG > NC_000913/4033480‑4033747 | gggAGCCACTACGCTGGTGTGGCTGGACTCGCTCCCCCACGCCATCCTCTGTGATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGTGTGGGATGCAGc < 1:151836/143‑1 (MQ=255) cACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTGTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGCTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGGGGGATGCAGCTCTATc > 2:195254/1‑143 (MQ=255) cTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGACTTGGATGCAGCTCTATCGCGCAGAAATgcccgg > 1:536515/1‑143 (MQ=255) aTTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATgcccggcccg > 1:334849/1‑143 (MQ=255) tCCTTCCCGCCATCCTATTGTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATGATCGTGTTGGCGGTTGCGATACTGCCTGTACTCGGCGTGAGTGGGATGCAGTTCTATCGCGCAGAAATGCCCGGCCCGCTGaaa < 1:446436/143‑1 (MQ=255) tCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTAGCCGTAAGAGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATa > 1:443268/1‑143 (MQ=255) aTCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGCTACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCCCAGAAATGCCCGGCCCGCTGAAAGATAACAAAAt > 1:403814/1‑143 (MQ=255) tCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTCTCCTCGGCGTGAGGGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGGTAACAAAATg > 1:524144/1‑143 (MQ=255) tctTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGTGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAGAATgcgc > 1:150163/1‑143 (MQ=255) ccAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGTGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGGTGAACGATTACAAAATGCGCCCGCGAAtgt > 2:195322/1‑141 (MQ=255) cAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGTGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGc < 1:257591/143‑1 (MQ=255) ttGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAACTGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGCAACGGCGAAAAcc > 1:143611/1‑143 (MQ=255) tGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGGGGTTg > 1:362717/1‑143 (MQ=255) gATCATCGTGTTGGCGGTTGCGATACTGCCTAACCTCACCGTGAGTGGGATGCAGCGCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGAgtt > 1:158290/1‑143 (MQ=255) atcatcGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCt > 2:140372/1‑83 (MQ=255) atcatcGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCt < 1:140372/83‑1 (MQ=255) gATACTGCCGATCCTCGGCGTGTGTGGGGTGCAGCTCTATCGCGCAGAAACGCCCGGCCCGCTGAAAGACAACTAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCt < 2:46227/143‑1 (MQ=255) aaCTGTCGATCCTCGGCGTGTGGGGGATGCAGCGCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCtgt < 1:381807/142‑1 (MQ=255) gCCTATCCTCGGCGTGTGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGACTACCCTGTGGTTGATTTATGTCTTGCTGACCGTCTCCTGTccgc > 1:243005/1‑143 (MQ=255) tCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTtg > 1:499072/1‑143 (MQ=255) ggCGTGTGTGGGGCGCAGCTCTAGCGCGCAGCCATGCCCGGCCCGCTGAACGATAACAAAATGCGCTCGTGAATCGCGGAAAGGGCGAAGACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTtgtg < 2:142561/143‑1 (MQ=255) gCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGGAACGGCGAAAACCCAGTGGTTGAAGAATGTCTTGCTGACCGTCGCCTGCGCGCTGGCGtggtgg > 2:458912/1‑143 (MQ=255) | GGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGG > NC_000913/4033480‑4033747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |