| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 672,113 | T→C | 13.8% | D25G (GAT→GGT) | lptE ← | lipopolysaccharide assembly protein LptE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 672,113 | 0 | T | C | 13.8% | 67.6 / 3.5 | 35 | D25G (GAT→GGT) | lptE | lipopolysaccharide assembly protein LptE |
| Reads supporting (aligned to +/- strand): ref base T (13/17); new base C (3/2); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01 | |||||||||||
CTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTAC > NC_000913/671977‑672254 | cTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTACTGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCa > 1:198853/1‑143 (MQ=255)cTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGACCCGTTCGGATCGTCTGCGTCCAGGATCATGGCCTTCATAATGGAAGGAACCTTAGTGGTATCACGCa > 1:200557/1‑143 (MQ=255) gaCGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCCTACGGAACTGGTTACGCACCGCACCGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGATACCACGCAgg < 1:551104/142‑2 (MQ=255) aCGCGTGGTTTCTTTATCCAGCAACTCGACACCCTTCAGACGTAACTGGTGACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGAt < 1:128516/143‑1 (MQ=255) gTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCGGGATCATGACCTTCATAGTGGAAGGAGCCTGCGGGGTATCACGCAGACgacagca > 2:387200/1‑142 (MQ=255) aGCACCTCGACACCATTCAGACGTACCTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGt < 2:520644/143‑1 (MQ=255) tcagcTCGGCACCAGTCAGACGCACCTGGTTCCGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTCCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTg < 2:19956/139‑1 (MQ=255) cAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGa < 1:278944/143‑1 (MQ=255) ccATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTTATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGc < 2:214492/143‑1 (MQ=255) aTTCTGACGTAACTGGTGACGCACCGCGCGGCTGAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGTTAATCACCGccg < 2:44794/143‑2 (MQ=255) tCAGACGTAACTGGTTACGGACCGCACGGCTTAAGGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCaga < 1:341042/143‑1 (MQ=255) tCAGACGTAACTGGTTACGCACCGCACGGCATAATGGCCCGTTCGGATCGCCCGAGTCCCGGATCACGAGCTTCCCAACGGAAGGACCCTGCCTGGTACCACGCACATCCCACCCACACCCGGCGGCGACTAACCCCGCAaga > 2:101873/1‑143 (MQ=255) tCAGACATAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGGGATTAACACCGCCagc > 2:405535/1‑142 (MQ=255) ttACGCACCGCACGGCTTAATGGCCCGTTGGGATCACTTCAGTCCGGGATGATGACCTTCATAGTGGAAGGAACCGTCTTGGTATCACGCATAGGCCATCCACACCCGGCGGTGATTGACACCACCAGAGAGaacaaccacgt > 2:229934/1‑138 (MQ=255) accgcacGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCGAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAg > 1:106458/1‑143 (MQ=255) tctgCTTCATGGCCCGTTCGGATCGCCTGCGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATc < 1:73002/140‑1 (MQ=255) ccGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTtcctcc > 2:55411/1‑143 (MQ=255) tCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGCTGCCCGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGATGCAAGAAAGCGCACGCTTCCTCCggcg > 2:90986/1‑143 (MQ=255) aTCGCCTGAGTCCAAGATCATGACCTTCATAGTAGAAGGAACCTGCGTGGTATCACGCAGATGCTCGCCACACAAAGCGGAGAAAAAAACCGCCAGAGATAACATCAATGTTGCCAGCTATCGCAAGCTACCTCTCGCGCTTa > 2:66362/1‑143 (MQ=255) ggTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGa < 2:104773/142‑1 (MQ=255) ggATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCCTCCCGCCGCGCTTAGCCAACGACCAgct > 2:19748/1‑141 (MQ=255) tCAGGACCTTCATAGTGGAAGCAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTga < 2:19394/143‑1 (MQ=255) tCAGGACCTTCAGAGTGGAAGGAAGCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATGAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTga < 2:63509/143‑1 (MQ=255) tttaTAGTGGATGGACCTTGCGTTGTATCACGCAGATGCCAGCCACACCGGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTa < 1:454958/140‑1 (MQ=255) tCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTAc < 1:55411/143‑1 (MQ=255) tGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTa > 1:30528/1‑143 (MQ=255) atcTGCGTGATACCACGCAGGTGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTGCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGGTTACCTGGTACGTAAATc > 1:119567/3‑143 (MQ=255) tcTGCGTGATACCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCcgc > 2:168368/2‑97 (MQ=255) tcTGCGTGATACCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCcgc < 1:168368/96‑1 (MQ=255) tGCGTGGTCTGACGCAGATGCCAGCCCCGTGCGGCGTGGATTAACACCGTCAGAGATAACAACACTGTTGCCAGATATCGCACGCTTCCTCCCCCGCCTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACt < 2:140175/143‑1 (MQ=255) gTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTa > 1:438934/1‑143 (MQ=255) tGGTATCACGCATATGCCAGCCACACCCGGCCGTGATTAACACCGCCAGAGATAACAACACTGGTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTAc < 2:440153/143‑1 (MQ=255) tGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTAc > 1:510200/1‑143 (MQ=255) gaTCACGCAGATGCCAGCCACACCCGGCGGTGATTAGCACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTa < 1:436910/142‑1 (MQ=255) aTCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATAGCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTAc < 2:106458/143‑1 (MQ=255) | CTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTAC > NC_000913/671977‑672254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |