| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 94.9% | 160.2 / NA | 61 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (28/28); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTC > NC_000913/124105‑124379 | tggtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACCGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTa > 2:1072461/1‑143 (MQ=255)tggtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTa > 1:916254/1‑143 (MQ=255)tggtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GATa > 2:214308/1‑143 (MQ=255) gtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATCACGCCACGGGAGAGAGCAGGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAg > 1:1111756/1‑143 (MQ=255) gtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATAGCGCACTAG‑GTTAAg > 1:851950/1‑143 (MQ=255) aCGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAACAACATCGCGCACTAG‑GTTAAgcac > 1:907366/1‑140 (MQ=255) cGAAGAAAATCTACGCCCCACTCAAGAAAGCGCAGGACACCCCAGAACACACAACATCAATCCTTACTCATACCATTAAAGTTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAAc < 1:208335/143‑1 (MQ=255) gaagaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAGGGGAAAACCGTCGAGAACTAG‑GAGAAGAAAAAGaaaa > 1:296184/1‑141 (MQ=255) agaaAATCTACGCTGCATTCAAGAAAGCGCAGCAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAACAACATCGCGCACTAG‑GTTAAGAAAATGAACATg < 1:373548/143‑1 (MQ=255) gaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTCATCCTTGCTAATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GGTAAGAAAATGAACATgg > 1:44300/1‑143 (MQ=255) gaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATgg > 1:900204/1‑143 (MQ=255) gaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATGAAAGGTTACGGCATGGGCAACTCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATgg < 2:497111/143‑1 (MQ=255) aaaTCTACGCTGAATTCAAGAAAGCGCACGAAACCAAAGGCCACGCGACAGTAATCCTTGCTCATACCACTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGAc < 1:303670/143‑1 (MQ=255) aTCTACTCTGCATTGAAGAAAGGGCAGGAGACGAAAGGCAAAGCGACAGGAATCCTTGCTGATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACgg < 1:747875/143‑1 (MQ=255) tCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGgt < 2:769106/143‑1 (MQ=255) aCGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGAAAAGCGACTGTAATCCTTGCTCATACAATTACAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGtgtg > 1:213833/1‑143 (MQ=255) cGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATACTTGCCCATACCATTAGCGGTTACGGCATGGGCGACGCGGCTGCAGGTAAAAACGTCGCGCCCCGG‑GTTAGGACAGTAGACATGGAGGGagtgg > 2:510102/1‑142 (MQ=255) cTGTATTCAAGAAAGCGTAGGAAATCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGt < 1:500949/143‑1 (MQ=255) cTGCATTCAACAAAGCGCAGGAAACCCAAGGCAAAGCTACAGTAATCCTGGCTCATACCCTTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGt < 1:394302/143‑1 (MQ=255) cgctcgcAAGACGGCGCAGCAACCCTGAGTCAACGCGTCGGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCGGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTc < 2:778798/137‑1 (MQ=255) ttCAAGAAAGCGCAGGAAACCGAAGGCAAAGCGACAGTAATCCCTTCGCATACCATTCAACGTTACGGCACGGGCGACCCGGCAGAACGTAGCAACAGCGCACACCAG‑TACAACAAAAGGACCATGGCCGGTGTCCGACatcg > 2:849531/1‑141 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAACGCGCCGGTAAGCCTTGCTCATACCATTAAAGGATACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGAACTAG‑GGTAAGAAAAACAACATGGACGGAGCGCGGCataggag > 2:741747/1‑139 (MQ=255) agagagTGCAGGAAAACAAAGGCAATACGACAGTGATCCGTGCTCATACGAGTAGCGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAGCA‑CACAGCCCAGTCTTAATAAAATGAACATGGACGGTGTGCGTCATATCcgc < 2:499348/143‑1 (MQ=255) atgtcgaaGGAAACCACAGGCAAAGCGACAGTACGCCTTGCGCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACAGCGCGCACTAG‑GTTAAGAAAATGAACATGGCCGGCGTGCGTCATATCCGCGa < 2:400330/136‑1 (MQ=255) aaaGAGCAGGAAACCAAAGGCAAAGCGACAGTAATCCGTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCTGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGa < 1:130610/143‑1 (MQ=255) aaGGCAGAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTATGCGTCATATCCGCGACCGTTTCAATGTGCCg < 2:584551/143‑1 (MQ=255) aGGGAAAGCGACGGGAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAACCATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTAATAGCCGCGACCGTTTCAATGTGCCgg < 2:398445/143‑1 (MQ=255) ggCAAAGCGACAGTAATCCCTGCTCATTCCATTAAAGGTTCCGGCATGGTCGACGCGGCTGAAGGGAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGgt < 2:391658/143‑1 (MQ=255) gCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGGTTCAATGTGCCGgtg > 1:829514/1‑143 (MQ=255) gCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGa < 1:457189/143‑1 (MQ=255) cAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCa < 1:672986/143‑1 (MQ=255) aTCCTTGCTCATACCATTCAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAACCATCGCGCACTAG‑GTTAAGAAATTGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGatat < 1:599167/143‑1 (MQ=255) tCCTTGCTGCTTGCATTGTAGGTTGCGGCATGGCCCACGCGGAGGACTGTATATACGTCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATc < 2:180130/143‑1 (MQ=255) cTTGCTCATACCATTACAGGGTGCCGCAGGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGa < 1:943630/143‑1 (MQ=255) cTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGAGCACTAG‑GTAAAGAAAATGAACATGGACGGAGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTGCGATGCGCGTATCGa > 1:336610/1‑143 (MQ=255) gCTCATACCATTAAAGTTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaaa < 1:322201/143‑1 (MQ=255) cTCATACCATTAAAGGTTACGGCGTGGGCGACGCGGCTGAAGGTACAAACATCGCGCACTAG‑GTTAAGAAAATGAACAGGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaaaa < 1:98666/143‑1 (MQ=255) cTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGCTATCGaaaaa > 1:239810/1‑143 (MQ=255) gttAAAGGCTACGGCATGTGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTAATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTa < 2:553887/142‑1 (MQ=255) aTTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTa > 1:363168/1‑143 (MQ=255) ttAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTAc > 1:936014/1‑143 (MQ=255) aGGTGACGGCTTGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGTCGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCa < 2:539358/143‑1 (MQ=255) ggTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GGTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCAc > 1:436915/1‑143 (MQ=255) ggTGACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACGAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCAc < 1:848202/143‑1 (MQ=255) aCGGCATGGGCGACGCGGTTGAAGGTAAATGCATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTc < 2:38541/143‑1 (MQ=255) ggCATGTGCGACGCTGCTGAATGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGGCGGTGTTTGATGCAGATATCGAAAAACTGCCGTACATCACCTTccc < 2:238292/143‑1 (MQ=255) gCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATAGCGAAAAACTGCCGTACATCACCTTCCCg > 1:939099/1‑143 (MQ=255) gCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAGCTGGCGTACATCACCTTCCCGGAAGGt > 1:59988/1‑143 (MQ=255) gCGACGCGGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGt > 1:531003/1‑143 (MQ=255) cgcgGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCAGGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCAGCTTCCCGGAAGGTTCTg > 1:156544/1‑143 (MQ=255) gcgGCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAACACTGCCGTACATCACCTTGCCGGAAGGCTCTGa > 1:651223/1‑143 (MQ=255) cgGATGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGaa < 2:526559/143‑1 (MQ=255) gCTGAAGGTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCCCGACCGCTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCGGAaga > 1:371760/1‑143 (MQ=255) ccgacggTAAAAACAACGCGCACGAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTTTGAGGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAagag < 1:573158/138‑1 (MQ=255) aGGTAAACACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATa < 1:165500/143‑1 (MQ=255) gTAAAAACATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCCGATATCGAAAAACTGCCATACATCACCTTCCCGGAAGATTCTAAAAAGCATAcc > 1:706163/1‑143 (MQ=255) acaaCATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTAt < 1:857927/141‑1 (MQ=255) aaaCATCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAGGGTTCTGAAGAGCATACCTATc > 1:420902/1‑143 (MQ=255) tCGCGCACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCAc > 1:905572/1‑143 (MQ=255) gcACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCTACCGTTTCAATGTGCCGGTGTCTGATGCCGGTATCGCAAAACTGCCGTACATCACCTTCCCGGCAGGTTCTGAAGCGCATACCTATCTGCGCGCTc > 1:889303/1‑143 (MQ=255) gcACTAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTc > 1:669030/1‑143 (MQ=255) | TGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAG‑GTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTC > NC_000913/124105‑124379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |