| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 95.6% | 105.1 / NA | 46 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (26/17); total (29/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132647‑2132926 | ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCTGTAAAAGTGGAAGTACCCGGAGACTTTTTTCTGTGACGAGGTGTTCTGCATTTTACCGTAGCTGATATGCAGAATc > 1:853386/1‑143 (MQ=255)ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGTTCTGCCTTTCACCGTGTTTGTTCTGCAGGCTc > 1:132156/1‑143 (MQ=255) tGGTAGAGGGTAACCAGGTGATATTGTTGTGTGGCGCGGTCGAATTTGTCCTTGTGCTTGCGGTAAAAGTGTAAGTACCCGGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt < 1:268570/143‑1 (MQ=255) ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCgcc > 1:143049/1‑141 (MQ=255) ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTACTTGCGGTAACAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCTTTTCACCGTGAGTGATATGCAGGCTCTGCGTc > 1:608936/1‑143 (MQ=255) tAGAGGGTCACCGGCTGAGCGTTTTTGCTGGCGCGGTCGAATGTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 2:370259/143‑1 (MQ=255) tAGAGCGTCAACAGCTGCTATTTTTTGCTGGCGCGGTCGAATTTGTCCTTGTGCTTGCGGTAACAGTGGAAGTACCCGGAGAATTTTTTCGGTGCCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 1:1541/143‑1 (MQ=255) gagGGTAAACAGCTGATATTTTTTTTTTTTTTTTTCGAATTTGTCTTTGTGCTTGCGTTCCATGTGGAAGTACCCGGAGAATTTTTTCGGTGCCGAGGTGCTCTGCATTTCACCGTGATTGATTTGCTGCTTCTGCGTCGCct > 2:1099227/1‑143 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGCCGAGGTGATCTGCATTTCACCGGGATGGATATGCAGGCTCTGCGTCGCatctc > 1:534721/1‑142 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGAAGAGGTGATCTGCATTTCAACGTGATTGCTATGAAGGCTCTGCGTCGCcttct > 2:1019594/1‑140 (MQ=255) tAAACAGCTGATATTTTTTGCTGGCGCGGTCGAGTTTGTCTTTGTGCTTGCGGTAAAGGTGGAAGGACCCGGAGAATTTTTTCGGGGACGAGGTGATCTGCATTTCACCGTGGTGGATATGGAGGAGTTGCGTCGCctctggg > 1:20540/1‑140 (MQ=255) caaCAGCTGATCTTTGGTGCTGGCGCGGTCGAACTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTGCGGTGACGGGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa < 2:688535/142‑1 (MQ=255) aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa > 1:59577/1‑143 (MQ=255) aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGTTAAAAGTGGAAGTACCCGGAGACTTTTTTCGGTGACGAGGGGATCTGCATTTCACCGTGATTGATCTGCAGGCTCGCCGTCGCCTCTTCTACt > 2:757559/1‑143 (MQ=255) gCTGATCTTTTTTGCTGGCGCGGTCGATGTTGGCTTTGTGCTTGCGGTATAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc < 2:1114577/143‑1 (MQ=255) gCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGTGTCGCCTCTTCTACTTTc > 1:861595/1‑143 (MQ=255) tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTGGCCTCTGCTACTTTCAACGGtgccg > 2:30364/1‑139 (MQ=255) cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATATGCATTTCACCGTCATTGATATGCAGGCTCTGCGTCGCCTCTACTACTTTCCACCGTTCGCCGTa > 2:277692/1‑143 (MQ=255) tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTTATTGATATGCAGGCTCTGCGTCCCCTCCTCTACTTTCCCCGGTTCGCCGTAc > 2:420014/1‑143 (MQ=255) gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:342058/1‑143 (MQ=255) cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTGCGGTGACGAGGTGAGCTGCACTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc < 1:209991/143‑1 (MQ=255) aTTTGTCTTTGTGCTTGCGGTAAAAGTAGAAGGAACCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc > 1:768793/1‑143 (MQ=255) tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACCATcc > 1:477912/1‑143 (MQ=255) tCTTGGTGCTGGCGGTGAAAGTGGAAGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg < 2:202360/143‑1 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGCTgc > 2:552375/1‑142 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt > 1:210143/1‑143 (MQ=255) ataGTGGAAGTTCCCGGAGACGTTTTTCGCCGACGCTGTGATCTGCATTTCACCGTGATTGATATGCAAGCTCTGCGCCGTCTCTTCTACTTTCCACGGTTCACCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCt < 2:280092/141‑1 (MQ=255) aaGTGCCCGGAGAATTGTTTCGGTGACGAGGGGATCTGCATCTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc < 1:730390/143‑1 (MQ=255) aaGTACCCGGAGAATTTTTTCGGTGACGAGGTCATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc < 1:831299/143‑1 (MQ=255) aGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCGCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGGAGCTGTCGTACTCCCGTGCCGcc > 2:604469/1‑142 (MQ=255) caCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt < 1:964436/142‑1 (MQ=255) ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg > 1:860455/1‑143 (MQ=255) tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAGCGGCAGCAAGACGTCGTAATCCTGTGCCGCTTTGAGTTCGGTATc > 1:319703/1‑143 (MQ=255) ttttCGGTGCCGGCGTGAGCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTCTCCACGGTTCGCCGTTCTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg < 2:496778/143‑1 (MQ=255) tCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac < 1:307551/143‑1 (MQ=255) ccGGGGACGAGGTCAGCTGCCTTTCACCGTGATTGGTATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCGGGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac < 2:608527/142‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa > 1:973023/1‑143 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCCTTCAGTTCGGTATccacccaa > 2:650990/1‑136 (MQ=255) tGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGAATCGAACAAACACTCTTTg > 2:1031464/1‑143 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa < 2:1052137/143‑1 (MQ=255) cATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGGTGGCGGGAGCCTGTGCCGCGCTCAGTTCGGTATCGAACAAGCACTCTGTGAAACGcc > 1:206909/1‑143 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGGTCGGTATCGAACAAACACTCTTTGAAACGCCATCCCCAg > 2:492232/1‑143 (MQ=255) atatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCACCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTACCGCTTCCATTTCGGTATCCAACAAACACTCTTTGCAACACCACGCCCAGGTAAAGAc > 2:463766/1‑143 (MQ=255) atGCAGGCTCTGCGTCGTCTCTTCTACGTTCCACGGTTCGCCGGACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt < 2:608936/143‑1 (MQ=255) atGCAGGCTCGGCGTCGCCTCGTCTGCTGTGCACGGTTCGCCGTACTCCGCCACCATCCGCAGTAAGCTGTCGTACAACTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt < 2:845679/143‑1 (MQ=255) aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGAAGATGCCGGAAGCCTGTGCCGCTTTCAGTCCGGTAGCGAACCAACACGCTTCGACACGCCACGACCACGACAAGCCCCGGtt > 2:808791/1‑143 (MQ=255) | GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132647‑2132926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |