Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,634	A→C	T108P (ACC→CCC)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,634	0	A	C	98.2%	154.0 / NA	56	T108P (ACC→CCC)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (22/33);  minor base G (0/1);  total (22/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTA  >  NC_000913/3041496‑3041768                                                                                                                                           |                                                                                                                                       cATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAGCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCAt                                                                                                                                    >  1:173438/1‑143 (MQ=255)  nttGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGACCTGAAAAAATATGCGGTATTCTCTCCAGTGCCCATc                                                                                                                                   >  1:66250/2‑143 (MQ=255)  atggCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATc                                                                                                                                   <  1:1005675/140‑1 (MQ=255)      cGACGCAAAAAGTAAAATGTGGAGCACTTCACCTCAGTGCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCgcgc                                                                                                                               <  1:755993/143‑1 (MQ=255)       gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTCACGTGCCCATCGCGcc                                                                                                                              >  1:639784/1‑143 (MQ=255)          gCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAga                                                                                                                           <  1:1030916/143‑1 (MQ=255)             aaaGGTAAAATGTGGAGCAATTTGCGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacga                                                                                                                        <  2:981717/143‑1 (MQ=255)                    aaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCAAGGATTGAACGGTGCAGCGGGCGTGAGCGGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGGCAGACGAGGAGAgt                                                                                                                 >  1:1070156/1‑143 (MQ=255)                    aaaCGTGGAGCAACTTACGTCTCGTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCCTGAACCGCAGCTGACTGAACTGACAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCgt                                                                                                                 <  1:487932/143‑1 (MQ=255)                       tgtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGtgtg                                                                                                              <  2:830352/143‑1 (MQ=255)                            aGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAACTATGCGGTATTCTCTAAAGTGCCCATCACGCCAGACGACGAGCGTGTgctgct                                                                                                         >  2:951425/1‑143 (MQ=255)                              caagcgaCGTCTGTTCCGCGACGGCGATGGCTTTGCATGGAGTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                       <  1:449529/137‑1 (MQ=255)                                   tACGTCTGTTCCGCGACGGCGGTGGCTTTGCAGGGGTTGACCGGCGCAGCGTGCGTGGACCGCAGCTGACTGAACTGGAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGtt                                                                                                  <  2:95363/143‑1 (MQ=255)                                       tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                              >  2:163524/1‑143 (MQ=255)                                              cgcgACGGCGATGGCTTTGCTTGGATTGAACGGCGCAGCGTGTGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                                                                       >  2:1000568/1‑143 (MQ=255)                                              cgcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                                                                       <  2:601302/143‑1 (MQ=255)                                              cgcgACGGCGATGGATTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAGTATGCGGTATTCTCTAGAGTGCCCAGCGCGCCAGACGACGAGCGTGGGCGCCTTGGTGCTGCCgcatccga                                                                                       >  2:405776/1‑136 (MQ=255)                                              cgagaaggCGACGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAAGATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                                                                       <  1:790080/137‑1 (MQ=255)                                                 gACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgc                                                                                    <  1:926306/143‑1 (MQ=255)                                                       gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACCAGCGTGTGCTGCTTGGTTTTGACGGTTTTCACgcgcgcgc                                                                              >  1:1042438/1‑143 (MQ=255)                                                         tGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccg                                                                            >  1:19209/1‑143 (MQ=255)                                                          ggCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgc                                                                           >  2:682503/1‑143 (MQ=255)                                                           gCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCGGGTTTTCAGGCGcgcgccgcg                                                                          <  1:410360/143‑1 (MQ=255)                                                              ttGCGTGGCTTGAGCGGCGCGGCGTGCGTGATCCGTAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCGTCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                                                       <  2:173364/143‑1 (MQ=255)                                                                gCATGGATTGAACGGCGCAGCGGGCGTGAACCGCAGCTGACTGAACGGAAAAAATATGCGGTATTCACTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                                                                     <  1:135054/143‑1 (MQ=255)                                                                  aTGGATTGAACGGCGCAGCGTGCGTGACCCGCAGCTGACTGAACTGAAAAAATATGCGGTATGCTCTAAAGTGCCCATCGCGACCGACGACGACCGTGTGCTGGGTGGTGTTGCCGGATCTCTGGCGAACACCGCACTggagc                                                                   >  2:175920/1‑140 (MQ=255)                                                                  aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGGATTCGCTAAAGTGCCCAGCGCGCAAGACGACGAGCGTGTGCGGCTTGGTGTTGCCGGTGGTGAGGCTCGCTCCGTTCTGGcga                                                                   >  2:384339/1‑141 (MQ=255)                                                                     gATTGAAGGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtc                                                                <  2:622774/143‑1 (MQ=255)                                                                     gATTGAACGGCGTCGCGTGCGTGATCCGCAGCGGACTGAGCTGCCTAAATAGGCGGTAGTCTCTAAAGTGCCCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtc                                                                <  2:965399/143‑1 (MQ=255)                                                                     gATTGAACGGCGCATCGTGCGTGACCCGCAGCTGACTGAACGGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTCCAGGCGCGCGCCGCGCTGGCAAAtc                                                                <  2:347042/143‑1 (MQ=255)                                                                       ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtctc                                                              >  1:812788/1‑143 (MQ=255)                                                                       ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGCTGCCGGGTTTCAGGCGCGCGCCGCGCTGGCAACtctc                                                              >  2:813151/1‑143 (MQ=255)                                                                        tGAACGGCGCAGCGTGGGTGAACCGCAGCTGACTGAACTGAAAAGATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                             <  2:926147/143‑1 (MQ=255)                                                                          aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                           >  1:788147/1‑143 (MQ=255)                                                                          aaCGGCCCGGCGTGCGTGAACCGCAGCTGACTGAACTGAAGAAATATGCGGTATTCTCTGAAGTGCCCATCGCGCCAGACGGCGAGCGTGCGCTGCTTGGTGTTGCCGTTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                           <  2:1052282/143‑1 (MQ=255)                                                                           aCGGGGGAGCGGGCGTGAACCGCGGCGGGGTGAACTGAAAAAAGATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGGGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                                                          <  2:88436/143‑1 (MQ=255)                                                                                gggtggtgcgtgAACCGCTGCTGCCTGGAGTGAAAAGATATGCGGTGCGCTCTATAGTGGCCATCGCGCCAGACGGCGGGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                     <  2:1001841/138‑1 (MQ=255)                                                                                    cgtgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGc                                                 <  1:61441/143‑1 (MQ=255)                                                                                      tgcgtgAACCCCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGATCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCt                                               <  1:274286/143‑1 (MQ=255)                                                                                        cgtgAACCGCAGCTGACTGATCTGAAAAAATATGCTGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTc                                             <  1:576204/143‑1 (MQ=255)                                                                                           gAACCGCAGCTGGCTGACCTGAACAAAGAGGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGGGTTGCCGGTGTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaa                                          <  2:561997/143‑1 (MQ=255)                                                                                                gCAGCTGACTGAACTGAAAAGATCTTCGTGATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaaa                                     <  2:437749/143‑1 (MQ=255)                                                                                                   gctggctgAACTGAAAAAATATGCGGTATTCTCTAAAGTGCGCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTGTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAAc                                  <  2:748244/143‑1 (MQ=255)                                                                                                        tcaaactgaaAAAATATGCGGTATTCGCTAAAATGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCTGGTTTTCAGGCGCGCGCCGCGTAGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                             <  2:79499/140‑1 (MQ=255)                                                                                                         tgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTCGCGAACTGCCTTCGAAAGAAAAACAGGTAg                            >  1:281678/1‑143 (MQ=255)                                                                                                          gaacggaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGt                           <  1:1044537/143‑1 (MQ=255)                                                                                                              tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAGATCTCTTTAGCGAACTGCCTTCGAAAGAGAAACAGGTCGTCaaa                       >  2:568198/1‑143 (MQ=255)                                                                                                              tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGGACGAAAAACAGGTAGTCcaa                       >  1:187508/1‑143 (MQ=255)                                                                                                                aaaacaTATGCGGTAGTTTTCAACGGGCCCTTGGCGGCAGACGACGGGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGTGCGCCGCGCTGGCAAATCTATTTAGCGAACTGTCTTCGAAAGAAAAACAGGTAGTCAaaga                     <  2:295494/143‑1 (MQ=255)                                                                                                                 aaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGCAAACCACGCAGTCAaagaa                    >  2:27832/1‑143 (MQ=255)                                                                                                                  aaaaTATGCGGTATGCTCTAAAGTGCCCGTCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag                   <  2:88950/143‑1 (MQ=255)                                                                                                                    aaTACGCTGGATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTCGCCGGTCTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgc                 <  2:842104/143‑1 (MQ=255)                                                                                                                                 ctctAATGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGc    <  2:289896/143‑1 (MQ=255)                                                                                                                                  tctAAAGTGCCCATCGCGCCAGAAGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag                   <  2:614811/127‑1 (MQ=255)                                                                                                                                  tctAAAGTGCCCATCGCGCCAGAAGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag                   >  1:614811/1‑127 (MQ=255)                                                                                                                                   ctAAAGTGCCCATCGCGCCAGACGACGAGAGTCTGCTGGTTGGTGTTGCCCGTGTTCAAGCGCTCGCCGCGATGGCAACTCTCTTTAGCGAACTGCCTTCGGAAGCAAAACAAGGAGTGAAAGAAGGCGCGAacacgtcgcca  >  2:160498/1‑136 (MQ=255)                                                                                                                                           |                                                                                                                                       CATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTA  >  NC_000913/3041496‑3041768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑