Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 135,167 G→T T139N (ACC→AAC)  speD ← S‑adenosylmethionine decarboxylase proenzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913135,1670GT95.9% 134.2 / NA 50T139N (ACC→AAC) speDS‑adenosylmethionine decarboxylase proenzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (22/25);  minor base A (2/0);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

ATCTCATGGTCGATAAAGTGCTTCATACCGTTAATGTCGCGGGTAAAACCGCGCACGCGATAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGGTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTT  >  NC_000913/135029‑135303
                                                                                                                                          |                                                                                                                                        
aTCTCATGGTCGATAAAGTGCTTCATACCGTTAATGTCGCGGGTAAAACCGCGCACGCGATAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTaga                                                                                                                                      <  1:214874/143‑1 (MQ=255)
   tcATGGTCGATAAAGTGCTTCATACCGTTAATGTCGCGGGTAAAACCGCGCACGCGATAATCAATGGTTAAGATAACGGAATCAAGCAGATAGATCAGGTAATTAAACGCCTTAAGCGGAGAAATCACGCCGCAGTTGGAGAc                                                                                                                                   >  1:940749/1‑143 (MQ=255)
         tCGATAAAGTGCTTCATACCTTTAATGTCGCGGGGAAAACCGCGCACGCGAAAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAat                                                                                                                             <  1:932726/143‑1 (MQ=255)
             tAAAGTTCTTCATACCGACAATACCGCGGGTAGAACCGCGCACGCGATAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCg                                                                                                                         <  1:149037/143‑1 (MQ=255)
                  tGCTTCATACCGTTAATGTCGCGGGTAAAACCGCGCACGCGATAATCAATTGTTACGATATCGGACTCAAGCTGGTGGATCAGGAAATACAGCGCCTACAGCAGAAAAATCACGCCGCAGTTAGAGAAATGAATATCGGcgcg                                                                                                                    >  1:792889/1‑143 (MQ=255)
                    cTTCATACCGTTAATGTCGCGGGTAAAACCGCGCACGCGATAATCAATGGTTACGATAACGGACTCAAACAGGAGGATCAGGAAATAAAGCGCATTCAGCGGAGAAATCACGCCGCAGTTAGCGGAATCGATATCGGCGGGGa                                                                                                                  >  1:691631/1‑143 (MQ=255)
                     ttCATTCGGTTAATGGCGTCGGTAGAACCGCGCGCGCGATAATCGATGGTTACGATTTCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGaa                                                                                                                 <  2:411739/143‑1 (MQ=255)
                          aCCGTTAATGTCGCGGGTAAAAAGGGATTCGCGTCAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGATAc                                                                                                            >  2:680309/1‑143 (MQ=255)
                                   gTCGCGGGTAAAACCGCGCACGCGATAATCAATGGTTACGATATCGGACACAAGCTGTGGGATCAGGTAATTCAGCGCCGTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAGTATCGGCGCGGAAGGGACGTAAACCGc                                                                                                   >  2:766801/1‑143 (MQ=255)
                                    gcgcgGGTAAACCCGCGCACGCGATAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGcc                                                                                                  <  2:1063833/142‑1 (MQ=255)
                                      gcgGGTAAAACCGCGCACGTGATAATCTATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCtt                                                                                                <  2:483942/143‑1 (MQ=255)
                                         ggTAAAACCGCGCACGCGATAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGCCTTCAATACCGGCGCGGAAGGTACATAAACCGCCTTCAg                                                                                             >  2:292780/1‑143 (MQ=255)
                                          gTAAGACCGCGCTCGCGCTAATCAATGTTTACGATATCGGACGCAAGCTAGTGGACCAGGTAATTCAGCGCTTTCTGCGGAGATATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGAAAGGTACATAAACCGCCTTCAgg                                                                                            <  2:684539/143‑1 (MQ=255)
                                           tAAAAACGCGCACGCGATAATCTATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGa                                                                                           <  1:838655/143‑1 (MQ=255)
                                            aaaaCCGCGCACGCGATAATCAATGGTTACGATATCGGACTCAAGCTGCTGGGTAAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGATAAAGACTTCAATATCAGCGCGAAAGGTACATAAACCGCCTTCAGGag                                                                                          >  2:498351/1‑142 (MQ=255)
                                              aaCCGCGCACGCGATAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCACCTTAAGCGAAGAAATAAAGCAGAAGATAAAGACTACAATATCGTCGCGGAAGGTACATAAACAGCCTTCAGGATGa                                                                                        >  1:585930/1‑143 (MQ=255)
                                               aCCGCGCACGCGATAATCAATGGTTACGATATCGGATTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGAc                                                                                       <  1:625025/143‑1 (MQ=255)
                                               aCCGCGCACGCGATAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTAAGCGGAGAAATCAAGCCGGAGTTACGAACCACAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGAc                                                                                       >  1:1110441/1‑143 (MQ=255)
                                                 cgcgCACGCGATAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGAAtt                                                                                     >  2:341387/1‑143 (MQ=255)
                                                     taCGCGATAATGAATGGTTACGATATCGGACTCAGGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCg                                                                                 <  2:804381/142‑1 (MQ=255)
                                                      aCGCGTTAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAATCGCCTTCAGGATGACTTTCCgg                                                                                <  2:40046/143‑1 (MQ=255)
                                                        gatttAATCATTGGTCACGATATCGGACTCAAGCTGGTCGATCAGGTAATTCCGCGCCTTCAGCGGAGAAATCACGCCTCAGTTAGAGACTCCAATATCGGCGCGGAATGTACATAAATCGCCTTCAGGATGACTTTCCGggt                                                                              <  2:195794/139‑1 (MQ=255)
                                                           aTAATCAATTGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGggtagg                                                                           <  1:386296/143‑1 (MQ=255)
                                                            tAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTCGAGACTTTAATGGCGGCGCGGAAGGTACATAAACCGCCTTCAGCATGACTTGCCGgggaggt                                                                          >  2:969918/1‑143 (MQ=255)
                                                              atccaaggTTACTAGATCGGACTCAAGCTGGTGGTTCTGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTAt                                                                        <  1:146779/137‑1 (MQ=255)
                                                               tCAATTGTTACGATATCGGACTCAATCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATg                                                                       <  1:934491/143‑1 (MQ=255)
                                                                          gATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCCAacat                                                            >  1:916635/1‑143 (MQ=255)
                                                                            tatCGGGCGCAAGCTTGTGGATCAGGTAATTCTGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGTGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGa                                                          <  2:19040/143‑1 (MQ=255)
                                                                                      acgCTGGTGGATCAGGTAATCCAGCGCCTTCAGCGGAGAAATCACGTCGCAGTTAGAGACTTCAATAGCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCaa                                                <  1:407812/141‑1 (MQ=255)
                                                                                      aaGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGGTGACTTTCCGGGGAGGTATGTGCGCGAATATGACTTTTATCaa                                                >  1:284213/1‑143 (MQ=255)
                                                                                           ggtggATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATCTGACTTTTATCAAGATgg                                           >  2:348440/1‑143 (MQ=255)
                                                                                              ggATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCa                                        >  1:875634/1‑143 (MQ=255)
                                                                                              ggATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTCTATCAAGCTGGGCa                                        >  2:444302/1‑143 (MQ=255)
                                                                                                 tCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACg                                     >  1:569939/1‑143 (MQ=255)
                                                                                                    ggTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGAcc                                  >  1:585394/1‑143 (MQ=255)
                                                                                                     gTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCg                                 >  1:834377/1‑143 (MQ=255)
                                                                                                      tAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCACGGAACGTACATAAACCGCCTTCAGGATAACTTTCCGGGTCGATATGTACGCAACTATGACTTTTATCACAATAGGCAACAACCGt                                >  2:62783/1‑143 (MQ=255)
                                                                                                          tCAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCt                            <  1:655948/143‑1 (MQ=255)
                                                                                                           cAGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTg                           <  1:1046976/143‑1 (MQ=255)
                                                                                                            aGCGCCTTCAGCGGAGAAATCAGGCTGCGGTGCCAAACCACAAAATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGGAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTgg                          <  1:1055532/143‑1 (MQ=255)
                                                                                                            aGCGCCTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTAAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTgg                          >  2:654149/1‑143 (MQ=255)
                                                                                                                ccTTCAGCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCTCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGt                      <  1:569469/143‑1 (MQ=255)
                                                                                                                      gCGGAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCg                >  1:40092/1‑143 (MQ=255)
                                                                                                                        ggAGTAATCACGCCGCAGCTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCggg              <  1:1081040/143‑1 (MQ=255)
                                                                                                                        ggAGAGATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCggg              <  2:1099941/143‑1 (MQ=255)
                                                                                                                        ggAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGAc                                   >  1:498475/1‑122 (MQ=255)
                                                                                                                        ggAGAAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGAc                                   <  2:498475/122‑1 (MQ=255)
                                                                                                                         gagaAATCACGCCGCAGTTAGAGACTTCAATATCGGCGCGCAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTCGGCATGTACGCAAATATGACTCTTATCAAGATGGGCAACGACCGGTTCTGGCAGTCGGCCgggg             >  2:1077770/1‑143 (MQ=255)
                                                                                                                                  cgtcgcAGTTAGAGACTGCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGtt    <  1:292780/140‑1 (MQ=255)
                                                                                                                                    ccgcAGTTGGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGtttt  <  1:188390/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
ATCTCATGGTCGATAAAGTGCTTCATACCGTTAATGTCGCGGGTAAAACCGCGCACGCGATAATCAATGGTTACGATATCGGACTCAAGCTGGTGGATCAGGTAATTCAGCGCCTTCAGCGGAGAAATCACGCCGCAGGTAGAGACTTCAATATCGGCGCGGAAGGTACATAAACCGCCTTCAGGATGACTTTCCGGGTAGGTATGTACGCAAATATGACTTTTATCAAGATGGGCAACGACCGTTTCTGGCAGTGGGCCGGGGTGTTCTGTTTT  >  NC_000913/135029‑135303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑