Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,033,610 G→A G156S (GGT→AGT)  trkH → K(+) transporter TrkH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,033,6100GA95.3% 105.5 / ‑5.2 44G156S (GGT→AGT) trkHK(+) transporter TrkH
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (26/15);  total (27/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGG  >  NC_000913/4033470‑4033747
                                                                                                                                            |                                                                                                                                         
tGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGCTGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCgtgagt                                                                                                                                         >  1:573902/1‑143 (MQ=255)
    cACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGa                                                                                                                                     <  1:769118/143‑1 (MQ=255)
      cTACGGGAGACACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGAAACGGCTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGACACTGGGTATCCTCGGCGTGAGTGGGGTg                                                                                                                                   >  2:900211/1‑143 (MQ=255)
                  acaCGCCGGCGGGGCTGGCTTCGCTCCCTCCCGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATcgc                                                                                                                       <  1:199345/141‑1 (MQ=255)
                   tACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATcgcg                                                                                                                      >  1:1012995/1‑143 (MQ=255)
                   tACGCTGGTGGGGCTGGATTCGCTCCCACACGACATCTTCGTTTTTCGTCAGATGCTGCAATGGTTTGGCGGACTGGGGATCATCGTGTTGGCGGTTGCGCTACTGCCTATCCTCGGCGTGAGGGGGATGCAGCTGTATcggg                                                                                                                      >  2:82525/1‑141 (MQ=255)
                   tACGCTGCTGGGTCGTGAGTCGCTCCCTCGCGCCATCCTCTTTTGTCGCCAGATGCTGCAATGATGTGGCGCGATGGGGACCATCGTGTTGGCGGTTGCGATGCTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATcgcg                                                                                                                      <  2:1115716/143‑1 (MQ=255)
                              ggCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATgccc                                                                                                           >  2:758154/1‑143 (MQ=255)
                                   gATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGGTGCAGCTCTATCGCGCAGAAATgcccggccc                                                                                                      >  1:939522/1‑143 (MQ=255)
                                   gATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGAGCATCGCGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGAGGCAGCTCTATCGCGCAGCAATgcccggccc                                                                                                      >  2:217604/1‑143 (MQ=255)
                                    aTTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAAGGGTTTGGCGGGATGGGGATCATCGTGTTGGCCGGTGCCCTACGGCCTCTCCTCTGCGTGGGTGGGCCGCAGCTCCCTCGCGCCGCAATgcccgccccc                                                                                                     >  1:909342/1‑142 (MQ=255)
                                        gCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGa                                                                                                 >  1:174661/1‑143 (MQ=255)
                                              tCACGCCATCCGCTGTTATGGCCAGATGCTGCAATGGTTTGGCGGGATGGGGAGCATCGTGTTGGCGGTGGCGATACTGCCGATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATa                                                                                           <  2:978022/143‑1 (MQ=255)
                                               ctcGCCATCCTCGTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATaa                                                                                          <  1:1990/141‑1 (MQ=255)
                                                     aTCCTCTTTGATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGAGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAAt                                                                                    <  1:222423/143‑1 (MQ=255)
                                                      tCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGCCCCGCTGAAAGATAACAAAATg                                                                                   >  2:992426/1‑143 (MQ=255)
                                                                tCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACGGCCTATCCTCGGCGGGAGTGGGAGGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGGAGGACACCAAAATGAGACCGCGaa                                                                         >  2:772729/1‑143 (MQ=255)
                                                                 cGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTGTCCTCGGCGTGAGTGGGATGCAGCTCTATCAAAACGAGGCGCTCTTCCCGCTGAAAGATAACAAAATGCGCCCGCGaac                                                                        >  2:491724/1‑142 (MQ=255)
                                                                   ccAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGGGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCACGCTGAGAGAGGACAAAATGCGCCCGCGGATTg                                                                      >  2:972705/1‑143 (MQ=255)
                                                                    cAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGc                                                                     >  1:407308/1‑143 (MQ=255)
                                                                              aaTGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCCGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCg                                                           >  2:695057/1‑143 (MQ=255)
                                                                              aaTGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGACAACAAAATGCGCCCGCGAATTGCGGAAACGGcc                                                           >  2:929942/1‑142 (MQ=255)
                                                                              aaTGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACGGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCg                                                           <  2:318319/143‑1 (MQ=255)
                                                                               aTGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGGGGGATGCAGCTCTGTCGCGCAGAAGTGCGCGGCCCGCTGGAAGATAACGAAATGCGCCCGCGAATTGCGGAGACGGCgg                                                          >  1:526315/1‑142 (MQ=255)
                                                                                  gTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGCATTGCGGAAACGGCGaaaa                                                       >  1:267868/1‑143 (MQ=255)
                                                                                   tttGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGAGACTGCCGATCCGCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAAc                                                      <  2:674950/143‑1 (MQ=255)
                                                                                       gCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGGTAACAAAATGCGCCCGCGAATTGCGGAAACGGCGCAAACCCtg                                                  >  1:346255/1‑143 (MQ=255)
                                                                                          ggATGGGGAGCATCGTGGTGGCGGTTGCGATACTGCCTATCCTCGGCGTTAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTgg                                               <  2:882784/143‑1 (MQ=255)
                                                                                              ggggATCTTCGTGTTGGCGGTTGAGATACCGCCTCTCACGGGGGTCGGTGGGTTGCAGGCTTATGGAGCAGTCTCGCCCGGCCCGCTGAAAGATAACAACCTGGGTCGCCCCGTTGCGGAAACGGCGAAAACCCTGTGGTTGa                                           <  2:21158/143‑1 (MQ=255)
                                                                                                   tcatcGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGAGGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTAt                                      <  1:871666/143‑1 (MQ=255)
                                                                                                         tgtTGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCGCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATCACAAAATGCGCCCGCGAATTGCGGAAACGGCGGAAACCCCGTGGTAGAAGTATGTATTg                                >  2:183915/1‑143 (MQ=255)
                                                                                                            tGGCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCtgtctg                             >  1:990101/1‑138 (MQ=255)
                                                                                                             ggCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAACGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCAGCGGTTGATGGATGTCGTGCTGa                            >  1:124569/1‑143 (MQ=255)
                                                                                                             ggCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGa                            >  1:228884/1‑143 (MQ=255)
                                                                                                             ggCGGTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGAGGACCCCGCGGAAGCCCGGGGGTTGGTTTATGTCTTGCTGa                            >  1:1054860/1‑143 (MQ=255)
                                                                                                                ggTTGCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGCAACGGCGAAACCTCTGTTGTTGTCTTATGTCTTGCTGACCg                         >  1:96656/1‑143 (MQ=255)
                                                                                                                  ctgctacaCTGCCTATCCTCTGCGTGCGTGGGATCCAGGTCAATCGCGCACACATGCCCGGCCCTCTCAAAGATAACAAAATGCGCCCGCTAATTGCTGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTc                       <  2:1017194/136‑1 (MQ=255)
                                                                                                                    gCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGGAGTGCGGAAACGGCGAAAACCCTGTGGTGGATTTATGTCTTGGTGACCGgcgc                     >  1:401967/1‑143 (MQ=255)
                                                                                                                    gCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGc                     <  1:26595/143‑1 (MQ=255)
                                                                                                                    gCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGGGGTTGAGTTATGTCTTGCTGACCGTCgg                     >  1:393639/1‑142 (MQ=255)
                                                                                                                    gCGATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGAGCACGCGAATTGCGGAAACGGCGAAAACCGTGTGGTTGATTTATGTCTTGATGAcagactc                     >  2:950762/1‑137 (MQ=255)
                                                                                                                      gATACTGCCTATCCTCGGCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCt                   <  1:87015/143‑1 (MQ=255)
                                                                                                                           ggtcgtcccTCGGTGTGAGTCGGACGCAGCTCGATCGCGCAGGAATGCCCGGCCCGCTGAAAGATAACAAAATGCGTCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTgcg              <  2:960777/136‑1 (MQ=255)
                                                                                                                                      ggCGTGAGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTtgtg   <  1:58852/143‑1 (MQ=255)
                                                                                                                                       gCGTGACTGGGATGCCGCTCGCTCGGGCAGAAATGCCCGGCCCGCTGAAAGATAACCAAATGCGCCCGCGAATTGCGGAAACGGCGAGAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTgg  <  2:405019/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGG  >  NC_000913/4033470‑4033747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑