Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,184,543	C→A	P1100Q (CCG→CAG)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,184,543	0	C	A	100.0%	212.9 / NA	67	P1100Q (CCG→CAG)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/29);  total (38/29)

TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAT  >  NC_000913/4184405‑4184678                                                                                                                                           |                                                                                                                                        tGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATGTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                     >  1:137944/1‑143 (MQ=255)    ctgtcaaaCGCCGCATCCAGCCTGCTGACAAGATGGCAGGTCGTCACCGTAACAAGGGTGTAATTTCTAAGATCACCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                  <  2:371465/138‑1 (MQ=255)       ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                               >  1:701353/1‑143 (MQ=255)              ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                        <  2:381773/143‑1 (MQ=255)                gcaTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCTATGATATGCCTTACGATGAAACCTGTACGCCGGTAGACCTCGTACTGAACCAGCTGGGCGTACCGTCTCGt                                                                                                                      <  1:324721/141‑1 (MQ=255)                 tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGCACCAGCTGGGCGTACCGTCTCGTa                                                                                                                     >  2:131892/1‑143 (MQ=255)                  aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                    <  1:955751/143‑1 (MQ=255)                   tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                   >  1:1088276/1‑143 (MQ=255)                   tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                   >  1:585067/1‑143 (MQ=255)                    ccAGCCTGGTCACCCGCTCGCCGCTCGTCACGGTAACAAGGGTGTAATTTCTAAGCTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa                                                                                                                  <  2:832558/143‑1 (MQ=255)                     cAGCCGGGTGACAAGATGGCAGGTCGTCACGGTAAGAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                 <  2:1111928/143‑1 (MQ=255)                               aCAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAg                                                                                                       >  1:923657/1‑143 (MQ=255)                                    aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTCGGCGTACCGTCTCGTATGAACAGCGGTCAGATCCt                                                                                                  >  1:263858/1‑143 (MQ=255)                                         aGGTCGTCAAGGGAACAAGGGTGAAATTTCTAAGATCAACCCGATCGACGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCCGGCCGTACCGTCTCGTATGAACCTAGGGCAGATcccagaga                                                                                             >  2:685576/1‑137 (MQ=255)                                         aGGGCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAATCTGTGCCCCACAATCAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                             <  1:514475/143‑1 (MQ=255)                                          ggTCGTCACGGTAAGGAGGGTGGAATTTCTAAGATCAACCCGATCGAAGATATGCCTGACGATGAGAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAc                                                                                            <  1:330364/143‑1 (MQ=255)                                              gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCAACTCGGTGCGATTGCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                        <  2:491803/143‑1 (MQ=255)                                               tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAcc                                                                                       >  1:526972/1‑143 (MQ=255)                                               tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGAACAGCGGTGAGATCCTCCAAACCCAcc                                                                                       >  2:381507/1‑143 (MQ=255)                                                      aagatgggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGCAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGCTCCTCGAAACCCACCTGGGtgt                                                                                >  2:746165/6‑141 (MQ=255)                                                         aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTCTGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                             >  1:916621/1‑143 (MQ=255)                                                            ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTCCTGAACCAGCTGGGCGTACCGTCTCGTGTGCACATCGGTCAGATCCTCGACACCCACCTGGGTATGGCTGc                                                                          >  2:107506/1‑143 (MQ=255)                                                            ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                          <  2:628857/143‑1 (MQ=255)                                                             gtgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTGTGGCTGCg                                                                         >  1:71217/1‑143 (MQ=255)                                                                tAATTTCTAAGATCAACCCGATCGAAGATATGCCTGACGAGGAAAACGGTGCGCCGGTAGACATCGTACTGAACCAGCGGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                      <  2:328305/143‑1 (MQ=255)                                                                tAATTGCTAAGACCAACCCTATCGACGAGATGCTTTACGATGCAGACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGCCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                      <  2:267224/143‑1 (MQ=255)                                                                tAATCTCTAAGACCACCCGGTTGGAGGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                      <  1:32142/143‑1 (MQ=255)                                                                tAACTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                      <  2:1061780/143‑1 (MQ=255)                                                                  aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAGCAGCTGGGCGTACCGTCTAGTATGAGCATCGGTCAGAGCCTCGCAACCAACCTGGGGATGGCTGCGaaaag                                                                    >  1:84310/1‑141 (MQ=255)                                                                  aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                     >  1:1118886/1‑78 (MQ=255)                                                                  aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                     <  2:1118886/78‑1 (MQ=255)                                                                    ttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTa                                                                  >  2:19831/1‑143 (MQ=255)                                                                      cgcaGATCTTCCCGATCGATGCTATGCCTTACGATGATAACGGTACGCCGGTATACATCGTACTGAACCAGCAGGGCGCACTGTCTCGTTTGAACAGCGGTGAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc                                                                <  2:447674/140‑1 (MQ=255)                                                                      cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGGAAGGTaac                                                                >  1:529838/1‑141 (MQ=255)                                                                       tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGCCTGCGCAAGGTCTCg                                                               >  2:1109935/1‑143 (MQ=255)                                                                          gATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTGTGGCTGCGAAAGGTATCGGCg                                                            >  1:1074933/1‑143 (MQ=255)                                                                           gtCAACGCGATCGAAGATATGCCTTACGATGCAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                           <  2:292379/142‑1 (MQ=255)                                                                              aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                        <  1:280274/143‑1 (MQ=255)                                                                               aCCCGATCGAAGATGTGCCCTACGATGAAGACGGTACGCCGGTAGACCTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                       <  2:968202/143‑1 (MQ=255)                                                                               aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                       >  1:312646/1‑143 (MQ=255)                                                                                 ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTAAACCAGCTGTGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGAATGGCTGCGAAAGGTATCGGAAACCAGAt                                                     >  2:1104065/1‑143 (MQ=255)                                                                                   gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                  <  2:717668/96‑1 (MQ=255)                                                                                   gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                  >  1:717668/1‑96 (MQ=255)                                                                                   gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCa                                                   >  1:171054/1‑143 (MQ=255)                                                                                    aTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                  <  1:739019/143‑1 (MQ=255)                                                                                    aTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGTATGGCTGCGAAAGGTATCGGCGACAAGCTCaa                                                  >  2:632533/1‑143 (MQ=255)                                                                                        aaGATAGGCCTCACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAGCGGCGACAAGATCAACGcc                                              <  2:25560/143‑1 (MQ=255)                                                                                         aGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTCTCGGCGACAAGATTAACTCCa                                             >  1:146876/1‑143 (MQ=255)                                                                                          gATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTTTGGCTGCGAAAGGTATCAGCGACAAGATCAACGCCAt                                            >  1:51330/1‑143 (MQ=255)                                                                                             atGCCTTACGATGAAATCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGAGAAAGGTATCGGCGACAAGATCAACGCCATGCt                                         <  2:137944/143‑1 (MQ=255)                                                                                              tGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAACAGCTGGGCGTACCGTCTAGTATGAACATAGGGCAGATCGTCGAAACCCAACGGGGTAGGGCGGCGAAAGGTATCGGGGACGAGATCAGCGCCAGGcag                                        >  2:360198/1‑141 (MQ=255)                                                                                               gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGGACAAAGCCATGCCGa                                       >  2:880266/1‑143 (MQ=255)                                                                                                 ctcaCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                     <  1:841014/140‑1 (MQ=255)                                                                                                   tACGACGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAca                                   <  2:922361/143‑1 (MQ=255)                                                                                                      gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATACTAAAAcagca                                >  1:695395/1‑143 (MQ=255)                                                                                                       aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCAAGTATGAACAGCGGTCAGATCCGCGAAACCCACCTGGGCATGGCTGCCAAAGGTATCGGCGACAGGAGCAACGCCGTGCGGAGAcagccg                               >  1:517233/1‑141 (MQ=255)                                                                                                                       ggTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGCTCAACGCCATGCTGAAACCGCAGCACAGAGTGGCGAAAc               >  1:890442/1‑143 (MQ=255)                                                                                                                       ggTAGACATCGTACTGAACCAGCTGAGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAGCTGGGTATGGCTGCGAAAGGTATCGGCGCCAAGATCAACGCCATGCCGAAACAGCAGCCAGAGGAAGCGAAAc               >  2:221044/1‑143 (MQ=255)                                                                                                                        gTATACATCGTACTGCACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACt              <  2:853575/143‑1 (MQ=255)                                                                                                                           gACCTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg           <  2:761908/143‑1 (MQ=255)                                                                                                                              aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACGAGATCAACGCCATGCTGAACCAGCAGCAAGAAGTCGCGAAACTGCGCGa        >  2:884277/1‑143 (MQ=255)                                                                                                                               tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGACGTCGCGAAACTGcgcgca       >  1:82994/1‑141 (MQ=255)                                                                                                                                cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACCTCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGGGAAAGGTATCGGCGACAAGATGAACGCCATGCTGAAACAGGCACAAGAAGTCGCGAAACTGCGCGAAt      >  2:188743/1‑143 (MQ=255)                                                                                                                                cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCCTGCTGACACAGCCGCAAGACGTCGCGACACTCCGCGaac      >  1:191597/1‑142 (MQ=255)                                                                                                                                  tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGGAACTGCGCGAATTc    >  1:388657/1‑143 (MQ=255)                                                                                                                                  tACTGAACCAGCTGGGCGTACCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGTGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTc    <  1:501547/143‑1 (MQ=255)                                                                                                                                    cTGAGCCAGCTGGGCGGACCGTCTGGTAGGAACATCGGTCAGATCCTCGAAACTCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATAAACGCCAGGCTGAAACAGCAGCAAGAAGGCGCGAAACTGCGCGAATTCAt  <  2:476669/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                        TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAT  >  NC_000913/4184405‑4184678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑