Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,768	G→T	R6S (CGC→AGC)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,768	0	G	T	98.5%	205.4 / NA	66	R6S (CGC→AGC)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (41/24);  total (42/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGA  >  NC_000913/674633‑674903                                                                                                                                        |                                                                                                                                        ccATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTTGATTCAATCTCTTCCAGACTGTATTGc                                                                                                                                  >  2:126397/1‑143 (MQ=255) ccATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGc                                                                                                                                  >  1:433001/1‑143 (MQ=255)          gTCGACCAGAGGGATGGGGAAGCATAGGCTGGCAGTAATACTTCTCTTTGCTCTCGTCTGCGGTTACTTCAAGTGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATg                                                                                                                         <  2:803718/143‑1 (MQ=255)           tCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATgg                                                                                                                        <  1:934954/143‑1 (MQ=255)            cGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGc                                                                                                                       >  1:850164/1‑143 (MQ=255)             gACCAGAAGGAGAGGGTAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGGGCGCTTCTCGTCCCAATTAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCa                                                                                                                      <  2:927646/143‑1 (MQ=255)                  gAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCAGCCCACTCAAGCTGTCCTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAg                                                                                                                 >  1:939425/1‑143 (MQ=255)                    gcgATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCGTCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTg                                                                                                               <  2:498379/141‑1 (MQ=255)                          gggAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTg                                                                                                         <  2:210025/143‑1 (MQ=255)                           ggAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCGTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGt                                                                                                        >  1:423775/1‑143 (MQ=255)                           ggAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATTGCAGCCAGTGGTCCTgc                                                                                                        >  2:654972/1‑142 (MQ=255)                           ggAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGACCTGt                                                                                                        >  2:152435/1‑143 (MQ=255)                              ggctttgACTGGCAGTAATTCTTCTCTTTGCTCCCGGCTTCGGTTACTTCAAATTTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGtttt                                                                                                     <  2:54287/137‑1 (MQ=255)                              aGCTTAGACCGCCTGTACTACTTCTCTTTGCGCTCGTCTTCGGTTATTTCAAATTTGCGTTTCTCATCCCAATGAAGCTGTCCTTTGGATTGTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGtttt                                                                                                     <  2:1061370/143‑1 (MQ=255)                                cATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGCAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCCCTTGCATGGCAGCCAGTGGTCCGGTCTTCa                                                                                                   >  2:629373/1‑143 (MQ=255)                                 aTAGCCGGGCAGGAATCCTTCTCTGTGCTCTCGTCTTCGGTTACTTCAAATGGGCGCTGCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTTTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCaa                                                                                                  <  2:1070755/143‑1 (MQ=255)                                 aTAGACAGGGAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCaa                                                                                                  <  1:99690/143‑1 (MQ=255)                                     aCAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACg                                                                                              >  2:61285/1‑143 (MQ=255)                                         gCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTa                                                                                          >  2:1007278/1‑143 (MQ=255)                                           aGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGATAATCAGGGGCTGCCTGTGGTCCTGTTTTCAATACGTCTCCa                                                                                        >  1:464456/1‑143 (MQ=255)                                            gTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACaa                                                                                       >  2:562266/1‑143 (MQ=255)                                               aTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATAGGGGTAccagtg                                                                                    >  1:56001/1‑138 (MQ=255)                                                  cgtcgctTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAg                                                                                 <  2:1010622/138‑1 (MQ=255)                                                   ttCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATCCGGCTACAAATGTAGc                                                                                >  1:551772/1‑143 (MQ=255)                                                        tttGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCAAATGAAGCTGTACTTTGGATTCTATCTCTCACAGGCTGTTTTGCTGTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAACTGTAGCGTTGa                                                                           >  1:1114241/1‑143 (MQ=255)                                                            ctctCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAggtg                                                                       <  1:154968/143‑1 (MQ=255)                                                             tctcGTCTTCGGTTACTTCAACTGGGCGCTTCTCATCCCAATGAAGCTGTACTTTGGGTTCTATCTCTTCCGGGCTGTATCGCTCTTGCATGGCAGCGAGTGGTGCTGTTTTCAATACGGCTACAAATGTAGCGTTGAggtgg                                                                      <  2:189024/143‑1 (MQ=255)                                                             tctcGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACACATGTAGCGCTGacgcgc                                                                      >  2:449320/1‑138 (MQ=255)                                                               tcGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACACATGTAGCGTTGAGGTGGtt                                                                    >  1:45016/1‑143 (MQ=255)                                                                cGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGACTGCATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTAAAAATGTAGCGCTCAGGTGGttt                                                                   >  2:844591/1‑143 (MQ=255)                                                                  tCTTCGGTTACTTCAAATGTACGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGACCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGttttt                                                                 >  2:386291/1‑143 (MQ=255)                                                                   cTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTc                                                                >  1:232479/1‑143 (MQ=255)                                                                   cTTCGGTTACTTCAAATGGGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTc                                                                <  1:916887/143‑1 (MQ=255)                                                                    ttCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACTAATGTAGCGTTGAGGTGGTTTTTCa                                                               <  1:825235/143‑1 (MQ=255)                                                                             ttCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCCTGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTAAAAGTGGAATTTCAGATCCgcat                                                      >  1:416951/1‑143 (MQ=255)                                                                             ttCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTCCACATGTAGCGTTGAGGTGGTTCTTCAGATCCgcat                                                      >  2:760895/1‑143 (MQ=255)                                                                             ttCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCgcat                                                      >  2:778015/1‑143 (MQ=255)                                                                             ttCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCAAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGCGGTTTTTCACACCGgcat                                                      >  2:874106/1‑143 (MQ=255)                                                                             ttCAAATGTGCGCTTCTCATCACAATGAAGCAGGACTTTGGATTCTATCTCTTCCGAGCTGTATTGCTATTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGGTGAGGTGGGTTTTCCGaaacaaat                                                      >  2:116815/1‑136 (MQ=255)                                                                              tCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATGCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCCGCCAGTGGTCCTGTTTTCAATACGGCTCCAAATGTAGCCATGCGGTGGGTTGTCAGATTCgcaga                                                     >  2:47039/1‑141 (MQ=255)                                                                                  aTGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCgcatagcat                                                 <  1:61285/143‑1 (MQ=255)                                                                                   tgtgCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCgcatagcata                                                >  1:282372/1‑143 (MQ=255)                                                                                   tgtgCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCgcatagcata                                                <  2:802924/143‑1 (MQ=255)                                                                                       cgcTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTc                                                                >  2:1082768/1‑123 (MQ=255)                                                                                       cgcTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTc                                                                <  1:1082768/123‑1 (MQ=255)                                                                                          ttCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCGTCGCCCaaa                                         >  1:738337/1‑143 (MQ=255)                                                                                            ctcATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCCAACg                                       >  1:223928/1‑143 (MQ=255)                                                                                               atctccgtGATGCTGTTCTTTTTTTTCTATCTCTTCTCGGCTTTATTGCTCTTGCATGGCAGCCAGGGGTCCTGTTTCCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTcc                                    <  2:846505/136‑1 (MQ=255)                                                                                                tCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCACTCCGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGCTCCGCATAGCATAGACCACACGTCcg                                   >  2:249176/1‑143 (MQ=255)                                                                                                      tGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCAGAGCCCAAAAGGCGGCGTCaa                             >  2:150173/1‑143 (MQ=255)                                                                                                          gCTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCGGCCAGTGGTCCTGTTTTAACTACAGCTACAGATGTCGCCGACGGCCAGGGTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCaaaccc                         >  2:1090373/1‑140 (MQ=255)                                                                                                           cTGTACTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTAAATCAGGCTACTAAAGTAGACTCCACATCGGTCTTCAGATCCGCATAGCATAGCCGAAACGTCAGCGTCAAAACAg                        >  1:208932/1‑143 (MQ=255)                                                                                                               acgttGGATTCTATCTCTTCCGGGCTGTACTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCtt                    <  1:685728/140‑1 (MQ=255)                                                                                                                cTTTGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGGCttt                   >  1:967980/1‑143 (MQ=255)                                                                                                                 tttGGATTCTATCTCTTCCGGGCTGTCTTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGGTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCCAACGTCCGGTTCCAAACAGCCttcc                  >  2:1105608/1‑141 (MQ=255)                                                                                                                 tttGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTGTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTc                  >  1:876673/1‑143 (MQ=255)                                                                                                                  ttGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTcg                 >  2:198438/1‑143 (MQ=255)                                                                                                                  ttGGATTCTACCGCTTCCGTCCTCTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCACACGTCCCCGTCAAAACAGCCTTTcg                 <  2:860938/143‑1 (MQ=255)                                                                                                                  gtGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTcg                 <  2:910711/142‑1 (MQ=255)                                                                                                                   tGGATTCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAGCGTCCGCGTCAAAACAGCCTTTcgc                >  2:725318/1‑143 (MQ=255)                                                                                                                       ttCTATCTCTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGGTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGGCAAAACACCCTTTCGCGCAc            >  1:266171/1‑143 (MQ=255)                                                                                                                            tctcTTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCggc       >  1:131588/1‑141 (MQ=255)                                                                                                                              tctTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGt     <  1:1076994/143‑1 (MQ=255)                                                                                                                               ctTCCGGGCTGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCAGCAAGTGCGCGTCAAAACAGCCTTTCGCGCACTCGACGtt    >  1:756663/1‑143 (MQ=255)                                                                                                                                gtCCGGGCTGTCTTGCTCGTGGATGTCAGCCGGTGGTCCTGTTTTCAATCCGGCTACAAATGTAGCGTTGAGGTTGTTTTTCAGATCCGCATAGCATAGCCCGAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTg   <  2:514952/142‑1 (MQ=255)                                                                                                                                 tCCGGGCTGTCTTGGTCTTGCATGGCAGCCAGTGGGCCTGTTTTCAATCCGGCTACAACTGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGa  <  2:683251/143‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGA  >  NC_000913/674633‑674903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑