Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	98.2%	152.3 / ‑6.2	55	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/32);  minor base G (0/1);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG  >  NC_000913/696332‑696606                                                                                                                                           |                                                                                                                                          tcccagccGACATGGAATGGTAAGACGGGTCAACATCGCAGTCGACATTGACTGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGaa                                                                                                                                       <  2:951573/137‑1 (MQ=37)  cGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAt                                                                                                                                      >  1:926395/1‑143 (MQ=255)     aTCCGACATTGAGGGATAAGACGTGTCAACATCGCATTCGACAGTGAAGGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCg                                                                                                                                  <  1:96270/143‑1 (MQ=255)          aCATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGcc                                                                                                                             <  1:243435/143‑1 (MQ=255)          aCATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGcc                                                                                                                             <  1:835477/143‑1 (MQ=255)              tGAAGTGTAAGACGTGTCAACAGCGCATTCGACATTGCATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTAc                                                                                                                         <  1:368985/143‑1 (MQ=37)               gAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTGATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTAcc                                                                                                                        >  1:563692/1‑143 (MQ=255)                   gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCtt                                                                                                                    <  1:461077/143‑1 (MQ=255)                    acaaGACGTGTCAACATCGCATTCGACATTGAACGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTg                                                                                                                   <  2:203814/141‑1 (MQ=255)                     tgaGATGTGTCAGCATCGCATGCGACTTTGCATGAACGCAGAAATGCATAACGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTgg                                                                                                                  <  2:492925/141‑1 (MQ=21)                     tacctcGTGTCAACAGCGCCTTCGGCATTGAATGAACGCAGAAAAGCAAAATGCTCGCCGAAGCGAGCTTTTCTAATGTGGCTTGTGTACGAGGAGTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTgg                                                                                                                  <  2:609948/138‑1 (MQ=25)                     tAAGACGTGTCAACATCGCATTCGACATTGACTGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTgg                                                                                                                  <  1:204273/143‑1 (MQ=255)                      aaGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGc                                                                                                                 >  1:287387/1‑143 (MQ=255)                         aCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGAt                                                                                                              >  1:163949/1‑143 (MQ=255)                         aCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGAt                                                                                                              <  1:545355/143‑1 (MQ=255)                          cGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATa                                                                                                             <  1:277931/143‑1 (MQ=255)                           gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCTAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCGTGGCGATAc                                                                                                            >  2:543617/1‑143 (MQ=255)                           gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATAc                                                                                                            <  1:548328/143‑1 (MQ=255)                            cgtCAACACCGCATTCGCCATTGAATGAGCGCAGAAAAGCAAATAGCTCGCCGCAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATAcc                                                                                                           <  1:493848/142‑1 (MQ=25)                             gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATAccc                                                                                                          >  2:829632/1‑143 (MQ=255)                              tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGGAT‑CCGGTGCCTTACCGCTTGGCGATGcccc                                                                                                         >  2:929969/1‑143 (MQ=255)                              tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CAGGTGCCTTACCGCCTGGCGATAccgc                                                                                                         >  2:365567/1‑141 (MQ=255)                                 aCATCGCTTTCGACATTGACAGCACGCAGGAGAGCAAACGGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAAc                                                                                                      <  1:413140/143‑1 (MQ=18)                                 aCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGCAT‑CCGGTGCCTTACCGCTTGACAATAGCCCAAc                                                                                                      >  2:605281/1‑143 (MQ=37)                                     cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTggg                                                                                                  >  1:580339/1‑143 (MQ=255)                                      gCATTCGACCTTGAATGAACGCAGAAAAGCAATACGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATTCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                                 <  2:364813/143‑1 (MQ=25)                                            gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGCGCATTACCGCTCGGCGATACCCCCAACAGGTGCACtc                                                                                           >  2:603905/1‑142 (MQ=25)                                             aCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCAAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCGTACCGCTTGGCGATACCCCAACAAGGTGCACTTa                                                                                          >  2:951100/1‑143 (MQ=25)                                               aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAcagaca                                                                                        >  2:920460/1‑138 (MQ=255)                                                 tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCCAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAAGGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                                                                                      <  1:966114/143‑1 (MQ=255)                                                     tgaaCGCAGAAGAGCAAAAAGCTCGCCGAAGCGAGCTGTTGTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCGACTGGGTGCACTTACAAGGTaa                                                                                  <  2:681841/143‑1 (MQ=25)                                                        aCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCAGCg                                                                               >  1:963252/1‑143 (MQ=255)                                                         cGCAGAAAAGTAAAAAGCTCGCCGAAGCGAGCTTTTTTACTGTGTCTGGGGGACGACGACTCCAACGATATTTTTTTTTGATTAGCATGCCTGT‑CCTTACCGCTTTGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt                                                                              <  1:259220/143‑1 (MQ=255)                                                          gCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAGCTGGGTGCACTTAACAGGTAAGCGTc                                                                             >  1:140410/1‑143 (MQ=255)                                                          gCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACCAGGTCAGCGTc                                                                             >  1:994998/1‑143 (MQ=255)                                                            aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTGAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                           <  1:1090543/143‑1 (MQ=255)                                                             gAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg                                                                          <  1:382284/143‑1 (MQ=255)                                                                aaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTTGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGAAACCCCAACTGGGTGCACTTACATGGTAAGCGTAtttacg                                                                       >  2:68905/1‑139 (MQ=25)                                                                    aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt                                                                   >  1:983931/1‑143 (MQ=255)                                                                     aaaaGGTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                  <  2:244265/143‑1 (MQ=255)                                                                        aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGc                                                               <  2:1078073/143‑1 (MQ=255)                                                                            cggcGAAGGGAGTTTTTTTAATGTGGCTGGGGTACGAGGTTTTTAACCTCGGAATGACGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                           <  1:967288/140‑1 (MQ=25)                                                                            cGCCGAAGCGAGCTGTTTGAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACTCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                           <  2:1051306/143‑1 (MQ=255)                                                                                  cgCGAGCTTCTTTAATGTGGCTGTGGTACGAGGACTCGAACGTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                     <  1:503101/142‑1 (MQ=25)                                                                                                   tgGCTGGGGTAGGGGGATTCGAGCCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCACTTGGCGATACCCCAACTGGGTGCGCTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGaa                                    <  2:555294/143‑1 (MQ=255)                                                                                                      cgggggGACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGc                                 <  1:741501/141‑1 (MQ=255)                                                                                                      cTGGGGCACGCGGACTCGAGCCTCGGAAGGCCGGAATTAGAAT‑CCGGTGCCTTACCACTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGc                                 <  2:545630/143‑1 (MQ=38)                                                                                                         gggTACGAGGATTCGAACCTGGGACTGCCGGAATTAGAAT‑CCGGGGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCgg                              <  1:270125/143‑1 (MQ=255)                                                                                                           gTACGGGGACTCCAACCTCGGAATGCCGGAACTAGAAT‑CCGGTGCATTACAGCTTGGCGATACCCCAAATGGGTGCACTTACACGGTAAGCGTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGaa                            <  2:69930/143‑1 (MQ=37)                                                                                                              ggAGGGTTCGCACCTCGGAATGCCGGAAGGAGACA‑CCGGCGCCGTACAGCTTGGCGATACCCCGACTGGGAGCACGCACGAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                         <  2:634224/142‑1 (MQ=21)                                                                                                                aGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGACCCTCGGTATGCCGGAATCAgc                       >  1:247918/1‑142 (MQ=255)                                                                                                                aGGATTCGAACCTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAGTCCGa                       >  1:267884/1‑143 (MQ=255)                                                                                                                           cTCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACACGGTACGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCGAACCTCGGAATGCCGGAATCAGCATCCGGTGCCt            >  1:260441/1‑143 (MQ=38)                                                                                                                            tCGGAATGCCGGAATTAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAATGGAAGCGCCTGCACTAAATTGGCAGGAGTACGTGGATACGAAGCAAGGAATGCCGGAGACGCAATCCggatgcga           >  2:644700/1‑137 (MQ=16)                                                                                                                                     gagAATTAGAAT‑CCGGTGCTTTACCGCTTGGCGAGACCCCAACTGGGTGCACGTACACGGTAAGGGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGGGCCTTACCGCTTgg  <  1:498709/141‑1 (MQ=25)                                                                                                                                           |                                                                                                                                          TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAT‑CCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG  >  NC_000913/696332‑696606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑