Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,446	A→G	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	96.1%	151.8 / NA	52	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (28/21);  minor base C (2/0);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754308‑754584                                                                                                                                           |                                                                                                                                           tCGCAGGACGCGACCGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGttt                                                                                                                                        <  1:992960/143‑1 (MQ=255)    cAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGc                                                                                                                                     >  1:876998/1‑143 (MQ=255)        gtGCGGGTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGCGCCGCAGCTGCCGTAATGACTGACTCTTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                                 <  2:1089888/142‑1 (MQ=255)            ggTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGAATATCAATAACACCTTGACCCAGCGTGCCTTTCAGCACATCCAGTCCAACAGCTGTATCCCCGTTGCGGGTGGGTTTTGCTTTTGTAt                                                                                                                             >  1:320791/1‑143 (MQ=255)              ttGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                           >  1:930223/1‑143 (MQ=255)              ttGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                           <  2:253435/143‑1 (MQ=255)                ggagtgaagCGTGGGTCCAAGGTGAACACACATTGTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGc                                                                                                                         <  1:92110/141‑1 (MQ=255)                   gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGCGAGTACTGATATTAATAACAGAAGGTCCCAGCGTGCCTTTCAGCACATTCAGTTCATCAGGTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAt                                                                                                                      <  1:414125/143‑1 (MQ=255)                      aagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCACTTTGAGGGTCGGCTTTGCTTTTGCAGCGGCCATCTa                                                                                                                   >  2:427272/1‑143 (MQ=255)                      aagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTCACCCAGCGTGCCTTCCAGCACATCCACTTCACCAGCGCTATCCCCGCTGAGCGTGGGTCTTGCTTTCGCATCTCCCAtttc                                                                                                                   >  2:149886/1‑142 (MQ=255)                        gCCTGGGGCAAAGGGGAACACACCTTTTGAACCGAGAGGACGGATAGCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                                 <  1:128946/143‑1 (MQ=255)                               tCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGCGGGTGGGATTTGCTTTTGTATCAGCCATTTAAGGTCTCCt                                                                                                          >  1:538268/1‑143 (MQ=255)                                caagtgTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCtt                                                                                                         <  2:835356/138‑1 (MQ=255)                                  aaGGTGAACACACCTTTTTAACAGAGAGTACTGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAg                                                                                                       <  1:123754/143‑1 (MQ=255)                                          cacacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAt                                                                                               >  1:618763/1‑143 (MQ=255)                                             acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGGTTCTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                            >  1:758325/1‑143 (MQ=255)                                             acCGTTTGAACCGAGAGTACGAATATCATTAACATCTTGATCCTGCGTGTCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                            <  2:824223/143‑1 (MQ=255)                                             acCCTTGGAACCGAGAGGACGGATATCATTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                            <  1:958145/143‑1 (MQ=255)                                                 gtcgAACCTAGAGTCCGTATATCTATATCATTTTTACCCATCGTGCCTTTCAGTTCATTCAGTTCAACAGCTTTATCCCCGTTGAGTGTTGGTTTTTCTTTTGTATCAGTCATTTAAGGTCTCCTTAGCGCCTTATTGCGTaa                                                                                        <  2:612077/140‑1 (MQ=255)                                                       ccGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGc                                                                                  <  1:544887/143‑1 (MQ=255)                                                          agagTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCGAGTTGAACAGCTGTATCCCAGTTGAGGGGGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGACGTGTTGCGTAGTACTGCCgg                                                                               >  1:1086695/1‑143 (MQ=255)                                                            agTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGaa                                                                             <  1:778487/143‑1 (MQ=255)                                                                   atatCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAt                                                                      >  2:867808/1‑143 (MQ=255)                                                                     atCAATAACAGCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAttt                                                                    <  2:717816/143‑1 (MQ=255)                                                                        aaTAACATCTTGACCCAGCGTGCCTTTCAGCCCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTCGTATCAGCCATTTAAGGTCCCCTTAGCGCCTTATTGCCTAAGACTGCCGGAACCTACATTTgac                                                                 >  2:377532/1‑141 (MQ=255)                                                                        aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGcc                                                                 >  1:899364/1‑143 (MQ=255)                                                                        aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTCGcc                                                                 >  2:695054/1‑143 (MQ=255)                                                                         aTAACATCTTGACCCAGCGTGCCTTTCAGCACACCCAGTTCAACAGCTGTATCCCCGTTGCGCGTGCGCTTTGCTTTTGTATCAGCCATTTCAGGCCTCCTTAGCGCCTTATTGCGTAAGACTGCCGACACTTACATTTGCCt                                                                >  1:32650/1‑143 (MQ=255)                                                                          tAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCtt                                                               >  1:374496/1‑143 (MQ=255)                                                                          tAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCtt                                                               >  1:718732/1‑143 (MQ=255)                                                                             cATCTTTACCCATCGTGCCTTTCAGCACATCCAGTTCAAAAGCTGTATCCCCGTTGAGGGGGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGc                                                            <  1:972102/143‑1 (MQ=255)                                                                             cATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGCGTTTTGCTTTTGTATCAGCCCTTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGGACTTACATTTGCCTTCGc                                                            >  2:371047/1‑143 (MQ=255)                                                                             cATCGTGACCCAGCGCGCCCTTCAGCACATCCAGTTCAACAGCTGTACCCCCGTTGAGGGTGGGTTTTGCTTTGGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGc                                                            <  1:790471/143‑1 (MQ=255)                                                                                     cccAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAATGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTCAATTTGCCTTCCCACATCGAc                                                    >  1:381070/1‑143 (MQ=255)                                                                                             gCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCttt                                            >  1:1085956/1‑143 (MQ=255)                                                                                                 ttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGCTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCCCATCAACCTGGCTTTcccc                                        >  2:891377/1‑143 (MQ=255)                                                                                                       acaTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAGCCgttgtt                                  >  1:499368/1‑143 (MQ=255)                                                                                                        caTCCAGTTCAACAGCTGTATCCCCGTTCCGGCTTTGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt                                 <  1:866656/143‑1 (MQ=255)                                                                                                            cAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                             <  1:944238/143‑1 (MQ=255)                                                                                                            cAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                             >  1:176167/1‑143 (MQ=255)                                                                                                               ttCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTATCCGCTTTTTATTTGGc                          >  2:389225/1‑143 (MQ=255)                                                                                                                  aaCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcg                       >  1:24143/1‑143 (MQ=255)                                                                                                                  aaCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACACGTTATTTATTTGGCTcg                       >  1:719678/1‑143 (MQ=255)                                                                                                                   aCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgc                      >  1:934165/1‑143 (MQ=255)                                                                                                                     aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTGAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTGCCCGTTTTTTATTTGGGTcgccg                    >  1:222983/1‑143 (MQ=255)                                                                                                                     aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgccg                    >  2:595267/1‑143 (MQ=255)                                                                                                                      gCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTGTCCTTAGAGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTCTTTTGCTcgccgc                   >  1:21410/1‑143 (MQ=255)                                                                                                                              cccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTCTCCGCATGATATGTGTCTCGCCGCTCTGTGac           >  1:997509/1‑142 (MQ=255)                                                                                                                                  ttGACGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAagag       <  1:834099/143‑1 (MQ=255)                                                                                                                                   tGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTCAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGGTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg      <  1:136946/143‑1 (MQ=255)                                                                                                                                     aGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgggg    <  1:734428/143‑1 (MQ=255)                                                                                                                                       ggTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGaa  <  1:159111/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                           TCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754308‑754584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑