| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 96.0% | 137.6 / ‑5.9 | 50 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (25/23); minor base C (1/1); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACACGC > NC_000913/760348‑760626 | aggtgcggaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAGGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGt < 2:310286/136‑1 (MQ=255) agctGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCAGCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTg < 1:503559/140‑1 (MQ=255) gAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTGGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGGTGGACTGGAGCGGCACGGAAAAcc > 2:302343/1‑143 (MQ=255) tgcggCTGCAGGAGCTGGCGAAAGGCATCAGCACGGTGCCGGAAGCAGGTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTc < 2:68677/142‑1 (MQ=255) aGCGCCTGCAGCAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTc < 1:20179/143‑1 (MQ=255) gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCg > 1:1079108/1‑143 (MQ=255) gcCCGCAGGAGCCGGCGAAACGCCTCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAACATTTATGGCGATCGCCACGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCt < 1:844596/143‑1 (MQ=255) gCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACg < 2:627957/143‑1 (MQ=255) aGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGcc > 1:401584/1‑143 (MQ=255) gAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGAGTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCCTACGGCAc > 2:237883/1‑143 (MQ=255) gCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCTGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGc < 2:974132/143‑1 (MQ=255) ggCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTgg > 1:290532/1‑143 (MQ=255) gCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGaa < 2:13068/143‑1 (MQ=255) gCACCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGaa < 2:291667/143‑1 (MQ=255) cATCAGCACGGTGCCGGAAGCAGTTGCAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCGAGGCGCTGGCTGCCGGTGAGCAACTGTTCGACTGGCGCGGTGCGGAAAACCTCGCTTACGCCCCGCTGGTTGACGAAg > 2:477858/1‑143 (MQ=255) cACGGTGCCGGAAGCAGGTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAATACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTc < 1:723694/143‑1 (MQ=255) ccGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGAGTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGCTGAAGGCCTTCCGGCTCg > 1:976324/1‑143 (MQ=255) cGGACGCAGGTGAAATGCAGTCTCGCGGTGCCAGGATTTAGGGCGAGCGCCAGGCGAGGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc < 2:358645/143‑1 (MQ=255) cGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc > 1:642412/1‑143 (MQ=255) ggAGGCAGTTGAAATGCAGTCTCGCGTGGCCAAGATGTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGcc < 2:638715/143‑1 (MQ=255) gCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGACAACCTCGCTTACGCCACGCTGGTTGTTGACGGCATTCCGGTTCGCCTGTc > 1:186608/1‑143 (MQ=255) aGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGCCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg < 1:485853/143‑1 (MQ=255) gTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTCGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGCAGGCATTCCGGTTCGCCTGTCggg > 1:415420/1‑143 (MQ=255) gTTGAAATGCAGTCGCGCTTTGCCGAGATGTGTGGCGATCGCCAGGCGATGGCTGCCGGGGAGAAACTGTTCGACTGGAGCGGTGCGGAAACCGTCTCTTTCGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCggg < 2:913456/143‑1 (MQ=255) ttGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGCCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCggat > 2:186437/1‑141 (MQ=255) ttGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGATGATGAAGGCAGTCCGGTTCGCCTGTCGGGt > 2:338381/1‑143 (MQ=255) aaaTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGaa < 1:35092/143‑1 (MQ=255) aaTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCACGCGATGGCTGCCGATGAGAAACTGTTCGACTGGAGCGGGGCGGACAAACTCGCTTACGCCACGCTGGTTGATGCAGTCATTCCCGTTCTCCTGTCGGGTGTAg > 2:348206/1‑143 (MQ=255) aTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGa > 1:400928/1‑143 (MQ=255) ctctcGCCTTTCCAAGATCTATGCCGATCGCCAGGCGATGGCTGCCGGTCAGAAACTGTTCGACTGGAGCGTTGCGGAAAACCTCGCTTACCCCACGCTGGTTGATTAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCg < 1:435548/142‑1 (MQ=255) ccAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAcc > 1:957467/1‑143 (MQ=255) ccAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAcc > 1:806174/1‑143 (MQ=255) ttATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACATTCTTcc < 2:553440/143‑1 (MQ=255) tGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAcc < 1:1087499/143‑1 (MQ=255) ggCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTGGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAGTACTCCGGTCGCGGTACCTTCTTCCACCg > 1:564732/1‑143 (MQ=255) cGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGGTGAAGGCATTCCGGTTCGCCTGGCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCTCCGcc > 1:961087/1‑143 (MQ=255) ccgggCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCgg < 1:890368/140‑1 (MQ=255) aTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGGAAACCCCGCTGACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCGGGTCGCGGTACGTTGTGCCACCACCGGCCGGTGGTCca > 2:87086/1‑143 (MQ=255) ggCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAACGCCGCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCcaca < 2:179968/143‑1 (MQ=255) tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACaaac > 1:868208/1‑141 (MQ=255) gagaAGGTGTGCGACTGGAGCGGTGCGGAAAGCCTCGCTCACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTaa < 2:221267/143‑1 (MQ=255) aggaaCTGGTCGACTGGAGCGGTGCGGAAAGCCTCGCGTACGCCACGCTGGTGGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAAc < 1:346481/140‑1 (MQ=255) aaCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGt > 1:594268/1‑143 (MQ=255) aCTGTTCGACTGGAGCGGTGCGGAAAACCTCTCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGtt > 1:833098/1‑143 (MQ=255) ttCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCAc > 1:745678/1‑143 (MQ=255) tCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCCGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCGTCCACCGCCACGCGGTGATCCACAACCCGTCCAACGCGTCCACt > 2:539822/1‑143 (MQ=255) cGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACtt < 2:290532/143‑1 (MQ=255) cTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGACGGCATTCCGGCTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTcca > 1:399244/1‑143 (MQ=255) tGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCg < 1:730958/101‑1 (MQ=255) tGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCg > 2:730958/1‑101 (MQ=255) cggCGGTGCGCACGTCCTCGCTTACGCCCCGCTGGTTGATGAAGGCGTTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAcgc < 2:77470/142‑1 (MQ=255) | AAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACACGC > NC_000913/760348‑760626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |