Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,151,736	T→C	V41A (GTT→GCT)	acpP →	acyl carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,151,736	0	T	C	97.7%	132.3 / NA	45	V41A (GTT→GCT)	acpP	acyl carrier protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (24/19);  minor base A (0/1);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATAGGAAATTTAAGAGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGTTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTC  >  NC_000913/1151598‑1151871                                                                                                                                             |                                                                                                                                         aTAGGAAATTTAAGAGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAg                                                                                                                                      >  2:466606/1‑143 (MQ=255)   aGGAAATTTAAGAGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCt                                                                                                                                    <  1:139546/143‑1 (MQ=255)     gAAATTTAAGAGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTgg                                                                                                                                  >  2:987203/1‑143 (MQ=255)     gAAATTTAAGAGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTgg                                                                                                                                  <  1:680979/143‑1 (MQ=255)               cgTATGAGCACCATCCAAGACCGCGTTAAGAAAATTAAAGGAGATGAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGctct                                                                                                                        <  1:93780/142‑1 (MQ=255)                   tGAGCACTATCGAAGAAGGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGGTAAGCAGGAAGAAGTTAACTGCAATGCTGGCTCGCTGGAAGGCGTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGgaa                                                                                                                    <  2:1091526/143‑1 (MQ=255)                   tGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGgaa                                                                                                                    >  1:983345/1‑143 (MQ=255)                   tGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGAGTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGgaa                                                                                                                    >  1:904738/1‑143 (MQ=255)                          tgtCGAAGGACTCGTTAAGACAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGt                                                                                                            <  2:417749/141‑1 (MQ=255)                          tATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGTCCGGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTCATGGCGCTGGAT‑GAAgtgt                                                                                                            >  2:188561/1‑143 (MQ=255)                          tATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAC‑GAAGAGt                                                                                                            >  2:888889/1‑143 (MQ=255)                          tATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTAACAATAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGt                                                                                                            >  1:1003995/1‑143 (MQ=255)                                       gTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATGCTGAGAt                                                                                               >  1:1021175/1‑143 (MQ=255)                                           agaagaTTAGCGGCGAACAGCGGGGCGTGAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCg                                                                                           <  1:1008202/143‑1 (MQ=255)                                           aGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCg                                                                                           >  1:895018/1‑143 (MQ=255)                                            gAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAGTGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCgg                                                                                          >  2:159402/1‑143 (MQ=255)                                             aaaaTTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTATTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATCGCTCTGGAA‑GAATAGTTTGATACTGATATTCAGGa                                                                                         >  2:417826/1‑143 (MQ=255)                                               aaTTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACACTGCTTCTTTCGTTGACGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACg                                                                                       >  1:137364/1‑143 (MQ=255)                                                actctCGGCGAACAGGTGGGCGTTAGGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGCGCTGGCAACGGCTATGGAA‑CGTTAGTTTGATACTGAGATTCCGGACga                                                                                      <  1:1123039/139‑1 (MQ=255)                                                      ggCGAACCGCTGGGCGTTAAGCAGGACGAAGTCACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGc                                                                                <  1:686622/143‑1 (MQ=255)                                                      ggCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTACGTACGAAGAAGc                                                                                >  2:830207/1‑143 (MQ=255)                                                         gagcAGCTGGGCGTTGAGCAGGAAGAAGGTACCAGCAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTGTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTga                                                                             <  2:1001896/140‑1 (MQ=255)                                                                          gCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGATGACCTGTGTGCTGTTTCTCTT‑TA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTc                                                            <  1:291905/143‑1 (MQ=255)                                                                           caagaagaaGTCACCAACAATGCTTCGTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCa                                                           <  1:553672/140‑1 (MQ=255)                                                                              gaagaaGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CAACGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGc                                                        >  1:703933/1‑143 (MQ=255)                                                                               aagaagTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCt                                                       >  2:906420/1‑143 (MQ=255)                                                                                         cagcaaTGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGAtt                                             <  1:622963/140‑1 (MQ=255)                                                                                         caacaaTGCTGCTTGCGTTGAAGACCTGGGCGCGGATTCTATTGGAGAACCGGT‑A‑TTTTTAA‑AGCAGTATAATGATGAGTTTTATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGAtt                                             <  2:235703/143‑1 (MQ=255)                                                                                                gCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCaa                                      <  1:90694/143‑1 (MQ=255)                                                                                                 cttcttTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTTAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACTAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAAc                                     <  2:197658/143‑1 (MQ=255)                                                                                                 cttcttTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAAc                                     >  1:827508/1‑143 (MQ=255)                                                                                                  ttcttTCGTTGAAGACCGGGGCTGCGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACCTCAACg                                    <  1:545414/143‑1 (MQ=255)                                                                                                       tCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGccac                               <  2:137364/143‑1 (MQ=255)                                                                                                          ttGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAg                            >  2:965974/1‑143 (MQ=255)                                                                                                          ttGAAGACCTGGGCGCGGATCCCCTT‑GA‑CACCGCTGAGCCGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAg                            <  1:61769/143‑1 (MQ=255)                                                                                                                aCCTGGGCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCCCGCTGCCATTGATTACATCAACGGCCACCAGGCGTaa                      >  2:601362/1‑143 (MQ=255)                                                                                                                    gggCGCGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGa                  >  1:980472/1‑143 (MQ=255)                                                                                                                      gcgcGGATTCTCTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAATATCACCACCGTTCAGGCTGCCATTGTTTACATCAACGGCCACCAGGCGTAAGTGAAc                >  1:98973/1‑143 (MQ=255)                                                                                                                              tctcTT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAg        >  1:236431/1‑143 (MQ=255)                                                                                                                                tctT‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGGAGCTGAGAAAAGCACCAGCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGc      >  1:914044/1‑143 (MQ=255)                                                                                                                                  tt‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCgg    <  1:348277/143‑1 (MQ=255)                                                                                                                                  tt‑GA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCACGCGTAAGTGAACCTCTCCAGGCgg    >  1:376931/1‑143 (MQ=255)                                                                                                                                     gA‑CATCGATGAGCTAGTAATGGATCTCGAA‑GAAGAGTTTGATACTGAGATTCCGGACGACGAAGCTGAGAAAATCCCCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTc  <  2:362619/143‑1 (MQ=255)                                                                                                                                     gA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACAt              <  2:438557/131‑1 (MQ=255)                                                                                                                                     gA‑CACCGCTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACAt              >  1:438557/1‑131 (MQ=255)                                                                                                                                             |                                                                                                                                         ATAGGAAATTTAAGAGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTT‑GA‑CACCGTTGAGCTGGTAATGGCTCTGGAA‑GAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTC  >  NC_000913/1151598‑1151871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑