Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	100%	intergenic (‑271/‑264)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	30 (1.100)	27/264	NT	100%	intergenic (‑271/‑267)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	32 (1.170)	30/264	NT	100%	intergenic (‑274/‑264)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979627‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/2101749‑2101890                                                                                                                                                                                                                                                                                              AATCACTCCCGGTGATCATATCATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGG                                                                                                                                               <  2:492679/143‑1                    ATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGG                                                                                                                            <  2:347334/143‑1                         TTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAGTAAGCGGGGAAAT                                                                                                                       >  2:179867/1‑143                                    TTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT                                                                                                            <  2:373228/143‑1                                            AAATAGTGTACTGATTCTCCGGATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGT                                                                                                    <  1:100850/143‑1                                                  TGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAACTTTTTCTCGGCTGACTCCGTCATTTC                                                                                              >  2:346776/1‑143                                                     ACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATT                                                                                           >  2:6953/1‑143                                                         ATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTT                                                                                       >  2:420069/1‑143                                                                   TTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCCTGTTTGtg                                                                             >  1:303550/1‑141                                                                    TAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC                                                                            <  2:360575/143‑1                                                                             GGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTT                                                                   <  1:367964/143‑1                                                                               TTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCT                                                                 <  1:153413/143‑1                                                                               TTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCT                                                                 <  1:320046/143‑1                                                                                TAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGCAGGTGAGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCAGTTCTTCATGTTTGAGCAGATTTTTTCTC                                                                >  2:271224/1‑143                                                                                  ATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTATTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCgc                                                              >  1:236798/1‑141                                                                                       TGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGTAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAA                                                         <  2:522456/143‑1                                                                                       TGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAA                                                         >  1:298918/1‑143                                                                                         TAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGCGCCGATTTTTTCTCCCGTAAATG                                                       >  2:362164/1‑143                                                                                                TTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAA                                                <  1:430934/143‑1                                                                                                TTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGAGCGAATAAGCGGGGCACTTCTTACCGGCGAACTCAGTCATTTCATTTCTTCCTGTTTGAGCAGATTTTTTCTCCCGTAAATGCCTTGAA                                                >  2:5407/1‑143                                                                                                           TTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAGTAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTT                                     <  1:179867/143‑1                                                                                                           TTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCGGCCTATTT                                     >  1:205598/1‑143                                                                                                                       TTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGCATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTT                         <  1:179912/143‑1                                                                                                                        TGCCTTTCTTTGTATTTAATTATGGAAGGTTCTAATAAGCGGGTAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCTTTTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTC                        <  2:248695/143‑1                                                                                                                        TGCCCTTCTGTGTATCTAATTAGGGAAGGGGCGAATAAGCGGGGAAATTCCTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGTTTTTTTCTCCCGTACATGCCTTGACTCAGCCTATTTAGACCGTTTCTTC                        <  1:510419/143‑1                                                                                                                          CCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGC                      <  1:62902/143‑1                                                                                                                                  TGTATTTAATTAGGGAAGGTGCGAATAAGCGGGTAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAG              <  2:65093/143‑1                                                                                                                                    ttTTTAATGAGGGAGGGTGCGAATAAGCGGGGAAATTCTTCGCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGC            <  2:205488/141‑1                                                                                                                                     cctgAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTGTCGGCTGACTCAGTCATTGCATTTCTACAGGTTTGAGCCGATGTTGTCTCCCGTAAATGCCGTGAGGCAGCCTATTTAGACAGTTTCTTCGCGATTTAAGGCG           >  1:55595/5‑143                                                                                                                                      TTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGACTCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGT          >  1:377983/1‑143                                                                                                                                        TAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCTCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTA        <  2:487683/143‑1                                                                                                                                           TTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGCTCAGCCTATTTAGACCGTTTGTTCGCCAGTGAGGGCGTTATgc     >  1:217557/1‑141                                                                                                                                           TTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGACTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC     >  1:203938/1‑143                                                                                                                                             AGGGAAGGTGCGAATAAGCGGGGAAATTCTTATCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATACCC   >  1:5917/1‑143                                                                                                                                             AGGGAAGATGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGGCGCTTTTTTCTCCCGTAAATGACTTGAATCAGCCTCTGTAGACCGCTTCTTCGCCATCTCAGGCGTCATACCC   >  2:12607/1‑143                                                                                                                                              GGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  <  2:160690/143‑1                                                                                                                                              GGGAACGTGCGAATAAGCTGGTAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  <  2:373131/143‑1                                                                                                                                                                                                                                                                                              AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979627‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/2101749‑2101890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.