| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | 100% | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 100.0% | 61.9 / ‑6.7 | 23 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (9/13); minor base G (0/1); total (9/14) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGC > NC_000913/124107‑124361 | gtaaCGATCCGAAGAAAATCTACGCTGCAGTCAAGAAAGCGCAGGAAACCAAACGAATATGGATAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAg < 2:321966/140‑1 (MQ=255) cACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATCCCATTCAAGGTTACGGCCTTGGCGACGCGGCTGAAGGTAAAAACAACACACAATGGGTTAAGaa > 1:44788/1‑143 (MQ=255) cGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaaa < 2:338909/143‑1 (MQ=255) caaTCTACGCTGCATTCAACAACGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGAc < 1:159264/142‑1 (MQ=255) cTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtg > 1:467578/1‑143 (MQ=255) tACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGATCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtgt < 1:305862/143‑1 (MQ=255) tCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATc > 1:50640/1‑143 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAACGCGACAGTAATCCTTGCTCATACCACTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGGTAAGAAAATGAACATAGACGGCGTGCGACATATGcg > 2:120935/1‑143 (MQ=255) aGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgc < 1:89700/143‑1 (MQ=255) cAGGAAACTAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGACGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGttt < 1:517943/143‑1 (MQ=255) aGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTc < 1:50958/143‑1 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTACGAAAATGGACAGGGACGGTGTGCGTCATATCCGCGACCGTTTCaa > 1:224108/1‑143 (MQ=255) aCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAtgt < 1:179729/143‑1 (MQ=255) tAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAGGGTAAAAACATCGCGCACTAGGGTAAGGAAAGGAACATGGACGGTGTGCGTCATATCCGCGACCGTGTCAATGTGCCGGTGTCTGATGCAGat > 2:307940/1‑143 (MQ=255) tggtCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAGCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaa < 2:410169/140‑1 (MQ=255) ttAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGATATGAAAATGAACATGGAAGGTGTGCGTCATATCCGCGACCGCTTCAATGTACCGGTGTCTGATGCCGATATCGAAACACTGCCgagt > 1:54522/1‑140 (MQ=255) gTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATAGCGAAAAACTGCCGTACATCAcc > 1:159485/1‑143 (MQ=255) ggCAGGGGCGACGCGGCGGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTccc < 1:226587/143‑1 (MQ=255) gcgttGGGCGACGCGGCTGAAGGTAAAAGCATCGCGCACGAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTccc < 1:357626/139‑1 (MQ=255) cATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCttggtgc > 1:473734/1‑138 (MQ=255) aTGGGCGGCGCGGCTGAAGGTAAAAACATCGCGCACTGGGTTAAGAAAATGAACATGGACGGTGTGCGTCATCTCCGCGTCCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGa < 2:496870/143‑1 (MQ=255) ggggCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTGAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGaa < 1:405555/142‑1 (MQ=255) tGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGGGCGTCATATCCGCGACCGTTGCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTCCTGAAGAGc < 1:501372/143‑1 (MQ=255) | GTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGC > NC_000913/124107‑124361 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |